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A New Strategy for Elimination of Lymphatic Filariasis-Development and Application of Diagnostic Methods Using Urine Samples

消除淋巴丝虫病的新策略——尿样诊断方法的开发与应用

基本信息

批准号：
12670243
负责人：
ITOH Makoto
金额：
$ 1.98万
依托单位：
Aichi Medical University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670243/
关键词：
filariasis diagnosis ELISA urine epidemiology

项目摘要

Development and standardization of a diagnostic method using urine samples to find out endemic foci and to evaluate control programs, which is essential for a global program to eliminate lymphatic filariasis' launched by WHO, is the main purpose of this study.1. Development of diagnostic method with urine samples for bancroftian filariasisWe developed an ELISA method using urine samples for bancroftian filariasis, which detect antigen specific IgG4 in urine. Both the sensitivity and specificity of the method were high; 96% and 99%, respectively. Little diurnal fluctuation of antibody titers and preservability of the urine samples after addition of sodium azide are advantage of this method for application to field studies.2. Application of the urine-ELISA to find out endemic foci in Sri LankaThe urine ELISA was used to find out endemic foci in a mountainous area in Sri Lanka where no information on filariasis was available. We examined 2,500 urine samples and found positives (4%). This … More positive rate was higher than the rate obtained by antigen detection kit with serum samples (0.6%) showing that the urine-ELISA is useful for finding endemic foci.3. Application of the urine-ELISA to evaluate a control program in ChinaThe urine-ELISA was used to evaluate a control program carried out in Yongjia in Zhejiang Province where no microfilaria positives has not been found for 10 years. All the samples collected from school age children, 2,800 samples, were urine-ELISA negative. Measurement of antigen specific IgG4 in urine samples from young generation with this method is considered a useful means for evaluation of filariasis control.4. Application of the urine-ELISA to another lymphatic filariasis, malayan filariasisThe urine-ELISA was used to detect antigen specific IgG4 in urine samples from residents in Brugia malayi endemic Islands in Korea. All microfilaria positives were urine-ELISA positive. All urine samples from young generation were negative, showing no recent transmission. Basic study to show sensitivity of the method for malayan filariasis is under study. Less

本研究的主要目的是发展和标准化一种利用尿液样本来发现地方性丝虫病疫源地和评估控制方案的诊断方法，这对WHO发起的全球消除淋巴丝虫病计划至关重要。班氏丝虫病尿液诊断方法的建立我们建立了一种检测班氏丝虫病尿液中抗原特异性IgG 4的ELISA方法。该方法的敏感性和特异性均较高，分别为96%和99%。抗体滴度的日波动小，尿样加叠氮化钠后的重复性好，是该方法应用于现场研究的优点.应用尿酶联免疫吸附试验（ELISA）在斯里兰卡一个无丝虫病资料的山区进行了丝虫病流行区的检测。我们检查了2,500份尿样，发现阳性（4%）。这 ...更多信息阳性率高于抗原检测试剂盒检测血清标本的阳性率（0.6%），说明尿液-ELISA对发现地方病疫源地有一定的帮助.应用尿酶联免疫吸附试验（urine-ELISA）对浙江省永嘉县10年来未发现微丝蚴阳性的微丝蚴病防治工作进行了评价。所有从学龄儿童收集的样本，即2 800个样本，都是尿液ELISA阴性。用本法检测青年人尿液中抗原特异性IgG 4，可作为丝虫病防治效果评价的一种有效手段.尿酶联免疫吸附试验（urine-ELISA）在另一种淋巴丝虫病--马来丝虫病中的应用本文应用尿酶联免疫吸附试验（urine-ELISA）检测了韩国马来丝虫病流行岛屿居民尿中的抗原特异性IgG_4。所有微丝蚴阳性者尿-ELISA均阳性。所有年轻一代的尿液样本均为阴性，表明近期没有传播。显示该方法对马来丝虫病敏感性的基础研究正在研究中。少