Establishment of bioartificial pancreas using PTD-FNK protein

利用PTD-FNK蛋白建立生物人工胰腺

• 批准号: 15590695
• 负责人: MIYAMOTO Masaaki
• 金额: $ 2.24万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590695/
• 关键词: Therapeutic Angiogenesis; DDS; bioartificial pancreas; PTD-FNK; アポトーシス; b-FGF; 糖尿病; 医療用ウジ治療; 医療用ウジ治療(MDT); ハイドロゲル; 骨髄幹細胞; stem cell

We investigated efficacy and safety of autologous bone marrow-mononuclear cells plus platelets including endothelial progenitor cells (EPCs) implantation for recovering refractory chronic peripheral arterial disease (PAD) using visual and quantitative analyses by ^<99m>Tc-tetrofosmin (TF) perfusion scintigraphy, and also investigated various quantitative assessments objectively. We performed consecutive twelve cases and 19 limbs and hands with severe chronic PAD that almost Fontaine class IV(11/12 cases, about 92%) in this trial. This treatment was very effective to relieve severe pain of PAD, especially for Buerger's disease. We used visual analog scale (VAS) for measurement of pain level. The maximum pain level before implantation was 66.5±5.0(mm), and decreased to 12.1±2.2(mm) after implantation (p<0.001). Rest pain in legs and fingers were resolved eleven cases (11/12 cases, 92%). All patients could measure pain-free walking time on treadmill were improved remarkably (before implan … More tation 140±53 sec versus after implantation 451±74 sec, p=0.034). And resting ankle-brachial pressure index (ABI) in legs implanted with bone marrow-mononuclear cells was also improved (before implantation was 0.65±0.08 versus after implantation was 0.73±0.07, p=0.055).According to ^<99m>Tc-TF perfusion scintigraphy, in proximal area (region from knee to ankle) before implantation was 1.32±0.10 versus after implantation was 1.56±0.11, p=0.007). ^<99m>Tc-TF perfusion scintigraphy, in distal area (region from ankle to end of toes, or from wrist to end of fingers) before implantation was 0.79±0.06 versus after implantation was 0.83±0.06, p=0.29). Ischemic legs and hands that were injected were increased perfusion blood flow. ^<99m>Tc-TF perfusion scintigraphy was effective to estimate visual and quantitative analysis of collateral vessels in neovascularization. Thus we successed to this new treatment for most severe chronic PAD unable to cure by present any treatments, this therapeutic angiogenesis would be a new strategy for saving severe ischemic limbs and hands. Less

我们通过^<99m>Tc-替曲膦（TF）灌注闪烁扫描进行视觉和定量分析，研究了自体骨髓单核细胞加血小板（包括内皮祖细胞（EPC））植入治疗难治性慢性外周动脉疾病（PAD）的有效性和安全性，并客观地研究了各种定量评估。本次试验连续进行了12例、19例肢体和手部几乎Fontaine IV级的严重慢性PAD（11/12例，约92%）。这种治疗对于缓解 PAD 的剧烈疼痛非常有效，尤其是对于布尔格氏病。我们使用视觉模拟量表（VAS）来测量疼痛程度。植入前最大疼痛水平为66.5±5.0(mm)，植入后下降至12.1±2.2(mm)(p<0.001)。腿部和手指的休息疼痛有 11 例得到缓解（11/12 例，92%）。所有患者在跑步机上测量的无痛步行时间均显着改善（植入前 140±53 秒与植入后 451±74 秒，p=0.034）。植入骨髓单核细胞的腿部静息踝臂压力指数（ABI）也得到改善（植入前为0.65±0.08，植入后为0.73±0.07，p=0.055）。根据^<99m>Tc-TF灌注显像，植入前近端区域（从膝盖到脚踝的区域） 为 1.32±0.10，植入后为 1.56±0.11，p=0.007)。 ^<99m>Tc-TF灌注闪烁扫描，在远端区域（从脚踝到脚趾末端的区域，或从手腕到手指末端的区域），植入前为0.79±0.06，而植入后为0.83±0.06，p=0.29）。注射的缺血腿和手增加了灌注血流量。 ^<99m>Tc-TF灌注闪烁扫描可有效评估新血管形成中侧支血管的视觉和定量分析。因此，我们成功地对目前任何治疗都无法治愈的最严重的慢性 PAD 进行了这种新疗法，这种治疗性血管生成将成为挽救严重缺血的四肢和手部的新策略。较少的

项目成果

医療用ウジを用いた多剤耐性緑膿菌(MDRP)合併糖尿病壊疽治療

医用蛆治疗多重耐药铜绿假单胞菌并发糖尿病坏疽

• 发表时间: 2006
• 期刊: 糖尿病 (印刷中)
• 作者: 藤本啓志;宮本正章 他
• 通讯作者: 宮本正章 他

宮本 正章: "重症難治性糖尿病性壊痕に対する自己骨髄細胞浸透人工真皮を用いた血管再生治療"Complication. 8. 31-37 (2003)

Masaaki Miyamoto：“使用自体骨髓细胞浸润的人造真皮对严重难治性糖尿病疤痕进行血管再生治疗”并发症。

Therapeutic angiogenesis by autologous bone marrow cell implantation for refractory chronic peripheral arterial disease using assessment of neovascularization by 99mTc-tetrofosmin (TF) perfusion scintigraphy

• DOI: 10.3727/000000004783983837
• 发表时间: 2004-01-01
• 期刊: CELL TRANSPLANTATION
• 影响因子: 3.3
• 作者: Miyamoto, M;Yasutake, M;Takano, T
• 通讯作者: Takano, T

今なぜ「医療用ウジ治療 (MDT) 」か?

为什么现在进行“医用蛆治疗（MDT）”？

• 发表时间: 2006
• 期刊: Pharma Medica 24
• 作者: 高木 元;宮本 正章 他;宮本 正章
• 通讯作者: 宮本 正章

自然免疫:自然免疫を応用した先端医療

先天免疫：应用自然免疫的先进医疗

• 发表时间: 2004
• 作者: Masaaki Miyamoto;et al.;宮本 正章;宮本 正章
• 通讯作者: 宮本 正章