Anamorsin, anti-apoptotic molecule, and hematopoiesis

阿那莫辛、抗凋亡分子和造血作用

基本信息

  • 批准号:
    15591006
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2004
  • 项目状态:
    已结题

项目摘要

Many growth factors and cytokines prevent apoptosis. Using an expression cloning method, we identified a novel antiapoptotic molecule named Anamorsin, which does not show any homology to Down apoptosis regulatory molecules such as Bcl-2 family, caspase family, or signal transduction molecules. The expression of Anamorsin was completely dependent on stimulation with growth factors, such as interleukin 3, stem cell factor, and thrombopoietin in factor-dependent hematopoietic cell lines, and forced expression of Anamorsin conferred resistance to apoptosis caused by growth factor deprivation in vitro. Furthermore, Anamorsin was found to act as an antiapoptotic molecule in vivo because Anamorsin -/- mice die in late gestation due to defective definitive hematopoiesis in the fetal liver (FL). Although the number of hematopoietic stem/progenitor cells in the FL did not decrease in these mice, myeloid, and particularly erythroid colony formation in response to cytokines, was severely disrupted. Also, Anamorsin -/- erythroid cells initiated apoptosis during terminal maturation. As for the mechanism of Anamorsin-mediated cell survival, a microarray analysis revealed that the expression of Bcl-xL and Jak2 was severely impaired in the FL of Anamorsin -/- mice. Thus, Anamorsin is considered to be a necessary molecule for hematopoiesis that mediates antiapoptotic effects of various cytokies.
许多生长因子和细胞因子阻止细胞凋亡。利用表达克隆的方法,我们鉴定了一种新的抗凋亡分子Anamorsin,它与Down凋亡调控分子如Bcl-2家族、caspase家族或信号转导分子没有任何同源性。Anamorsin的表达完全依赖于生长因子的刺激,例如因子依赖性造血细胞系中的白细胞介素3、干细胞因子和血小板生成素,并且Anamorsin的强制表达赋予对体外生长因子剥夺引起的细胞凋亡的抗性。此外,发现Anamorsin在体内充当抗凋亡分子,因为Anamorsin -/-小鼠由于胎肝(FL)中有缺陷的确定性造血而在妊娠晚期死亡。尽管这些小鼠FL中造血干/祖细胞的数量没有减少,但响应于细胞因子的髓系,特别是红系集落形成被严重破坏。此外,Anamorsin -/-红系细胞在终末成熟期间启动凋亡。至于Anamorsin介导的细胞存活的机制,微阵列分析显示Bcl-xL和Jak 2的表达在Anamorsin -/-小鼠的FL中严重受损。因此,Anamorsin被认为是造血的必要分子,其介导各种细胞的抗凋亡作用。

项目成果

期刊论文数量(48)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Identification of a cytokine-induced antiapototic molecule anamorsin essential for definitive hematopoiesis.
细胞因子诱导的抗凋亡分子阿那莫辛的鉴定对于确定的造血作用至关重要。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Shibayama H;et al.
  • 通讯作者:
    et al.
Strong correlation between the prevalence of cerebral infarction and the presence of anti-cardiolipin/b2-glycoprotein I and anti-phosphatidylserine
脑梗塞的患病率与抗心磷脂/b2-糖蛋白 I 和抗磷脂酰丝氨酸的存在存在强相关性
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    J.Nojima;et al.
  • 通讯作者:
    et al.
A.Kawasaki, et al.: "Opposing effects of PML and PML/RAR alpha on STAT3 activity"Blood. 101(9). 3368-3373 (2003)
A.Kawasaki 等人:“PML 和 PML/RAR α 对 STAT3 活性的相反作用”血液。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Suppressor of cytokine signaling-1 gene silencing in acute myeloid leukemia and human haematopoietic cell lines.
急性髓性白血病和人类造血细胞系中细胞因子信号传导 1 基因沉默的抑制剂。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    W.Dai;et al.
  • 通讯作者:
    et al.
H.Shibayama, et al.: "Identification of a cytokine-induced antiapoptotic molecule Anamorsin essential for definitive hematopoiesis"J.Exp.Med.. 199(4). 581-592 (2004)
H.Shibayama 等人:“细胞因子诱导的抗细胞凋亡分子对最终造血至关重要的阿那莫辛的鉴定”J.Exp.Med. 199(4)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

SHIBAYAMA Hirohiko其他文献

SHIBAYAMA Hirohiko的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('SHIBAYAMA Hirohiko', 18)}}的其他基金

Analysis of the roles of anti-apoptotic molecule, Anamorsin in immune cells
抗凋亡分子Anamorsin在免疫细胞中的作用分析
  • 批准号:
    25461449
  • 财政年份:
    2013
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of the roles of anamorsin, anti-apoptotic molecule, onsurvival and proliferation of B lymphocytes
抗凋亡分子阿莫辛对B淋巴细胞存活和增殖的作用分析
  • 批准号:
    22591034
  • 财政年份:
    2010
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Clarification of functions of Anamorsin, anti-apoptotic molecule, and its roles in malignant lymphomas
阐明抗凋亡分子阿那莫辛的功能及其在恶性淋巴瘤中的作用
  • 批准号:
    19591108
  • 财政年份:
    2007
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Clarification of mechanism of anamorsin, anti-apoptotic molecule
阐明抗凋亡分子阿莫辛的作用机制
  • 批准号:
    17590996
  • 财政年份:
    2005
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Pharmacologically-directed termination of anti-apoptosis to overcome drug resistance in acute leukemia.
药理学指导终止抗凋亡以克服急性白血病的耐药性。
  • 批准号:
    21K07239
  • 财政年份:
    2021
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Targeting drug tolerant persisters in colorectal cancer via anti-apoptosis inhibition
通过抗凋亡抑制靶向结直肠癌中的耐药持久者
  • 批准号:
    449631
  • 财政年份:
    2020
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Studentship Programs
Personalized targeting of anti-apoptosis pathways to overcome therapeutic resistance in melanoma
个性化靶向抗凋亡途径以克服黑色素瘤的治疗耐药性
  • 批准号:
    10733197
  • 财政年份:
    2017
  • 资助金额:
    $ 2.24万
  • 项目类别:
Control of beta-cell mitochondrial physiology and adaptation by core anti-apoptosis proteins
核心抗凋亡蛋白对 β 细胞线粒体生理学和适应的控制
  • 批准号:
    320311
  • 财政年份:
    2015
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Operating Grants
Humanized yeast and the study of anti-apoptosis
人源化酵母及其抗细胞凋亡的研究
  • 批准号:
    217502-2009
  • 财政年份:
    2015
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Discovery Grants Program - Individual
Humanized yeast and the study of anti-apoptosis
人源化酵母及其抗细胞凋亡的研究
  • 批准号:
    217502-2009
  • 财政年份:
    2014
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Discovery Grants Program - Individual
Humanized yeast and the study of anti-apoptosis
人源化酵母及其抗细胞凋亡的研究
  • 批准号:
    217502-2009
  • 财政年份:
    2013
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Discovery Grants Program - Individual
The molecularly-targeted therapy targeted at anti-apoptosis molecules Survivin for the pediatric malignant solid tumor
针对小儿恶性实体瘤的抗凋亡分子Survivin的分子靶向治疗
  • 批准号:
    24592695
  • 财政年份:
    2012
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Humanized yeast and the study of anti-apoptosis
人源化酵母及其抗细胞凋亡的研究
  • 批准号:
    217502-2009
  • 财政年份:
    2012
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Discovery Grants Program - Individual
Control of beta-cell mitochondrial physiology by core anti-apoptosis proteins
通过核心抗凋亡蛋白控制 β 细胞线粒体生理学
  • 批准号:
    238948
  • 财政年份:
    2011
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Operating Grants
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了