Induction of ultraviolet induced melanoma in the reconstructed skin using DNA repair deficient melanocytes

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1.We succeeded in culturing melanocytes derived from xeroderma pigmentosum type A patients who lacke DNA repair ability. Compared with normal human melanocytes, apoptotic change was easily induced by ultraviolet (UV)B irradiation at lower dose in xeroderma pigmentosum melanocytes, however, there was no difference recognized in case of UVA radiation.2.DNA repair deficient melanocytes with different melanin subspecies were established from XPA gene knockout mice with the genetic background of C57/B16, yellow, and albino mutant. XPA gene knockout albino mice were obtained from Prof. Kiyoji Tanaka in Osaka University and yellow, and black mice were established by us by backcrossing the mice.3.Reconstructed skin was made using normal human melanocytes, keratinocyte, and fibroblasts by Hat & Cap method on the back of SCID mice. Black colored human skin was successfully made, however, by histochemistry examination, human melanocytes were observed not in the epidermis, but in the dermis. This indicates that the addition of melanocyte surviving factor such as SCF (stem cell factor) might be required for maintenance of melanoyctes in the epidermis.