A role of osteopontin in ulcerative colitis

骨桥蛋白在溃疡性结肠炎中的作用

• 批准号: 15591446
• 负责人: MASUDA Hideki
• 金额: $ 2.11万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591446/
• 关键词: osteopontin; ulcerative colitis; inflammatory bowel disease; Crohn's disease; diverticulitis; oligonucleotide array; オステオポンテイン; 憩室炎

Ulcerative colitis(UC) is a multifactorial disorder of unknown etiology. Few studies have applied genome-wide gene expression analysis in colon tissue samples of UC. We performed the analysis of gene expression in UC and noninflamed control specimens using high-density oligonucleotide array system (Affymetrix GeneChip^<【○!R】> System, Santa Clara, CA). In addition, using the antibody against the gene product that was expressed most remarkably in the colon tissue of UC, immunohistochemistry(IHC) study was performed in colon tissues of ulcerative colitis(UC), Crohn's disease(CD) and diverticulitis(Div) patients. Methods : To compare differences in the level of gene expression between UC and control samples, 10 colonic tissue samples (7 UC and 3 normal control samples) were subjected to high-density oligonucleotide array analysis. In addition, IHC staining study for the remarkable expressed gene product was performed in three groups : UC group, CD(Crohn's disease) group, Div(diverticulitis … More ) group. Results : 1)Twenty five genes had a 3.0〜23.4-fold higher mRNA expression in UC samples compared with normal samples. Among the 25 genes, only two genes varied by more than 10-fold and a gene with the strongest expression was osteopontin(OPN). 2)Regarding OPN expression by IHC in intestinal epithelial cells, no difference was noted among the three groups. However, in the submucosa of the UC group, the ratio of two types of large cells (oval and spindle shape) expressing OPN was 61.2±14.4% (mean±SD), which was a significantly higher ratio than the CD group (14.9±7.0%) (p<0.05) and the Div group (11.2±6.1%) (p<0.05). In addition, the ratio of the UC group in the subserosa (50.1±15.0% )(mean±SD) was significantly higher than the CD group (16.9±6.2%) and Div group (12.6±5.7%). Based on the serial section study, oval shape cells were stained for anti-CD 68,while spindle shape cells were stained for anti-vimentin. Conclusion : Our present study indicates the possibility that osteopontin plays an important role in one of the pathogenesis of UC via increased immune activity. Less

溃疡性结肠炎(UC)是一种病因不明的多因素疾病。很少有研究将全基因组基因表达分析应用于UC的结肠组织样本。我们使用高密度寡核苷酸阵列系统(Affymetrix GeneChip^&lt；[0！R]&gt；System，Santa Clara，CA)分析了UC和非炎症对照标本中的基因表达。此外，利用在UC结肠组织中表达最显著的基因产物的抗体，对溃疡性结肠炎(UC)、克罗恩病(CD)和憩室炎(Div)患者的结肠组织进行了免疫组织化学(IHC)研究。方法：采用高密度寡核苷酸芯片技术对10例结肠组织标本(7例UC和3例正常对照)的基因表达水平进行比较。此外，对UC组、CD(克罗恩病)组、DiV(憩室炎…)组的显著表达基因产物进行了免疫组织化学染色研究更多)组。结果：1)25个基因在UC组织中的表达水平是正常对照组的3.0~23.4倍。在25个基因中，只有两个基因的差异超过10倍，其中表达最强的基因是骨桥蛋白(OPN)。2)肠上皮细胞IHC表达OPN，三组间差异无统计学意义。在UC组粘膜下层，卵圆形和梭形两种大细胞表达OPN的比例为61.2±14.4%(平均值±SD)，显著高于CD组(14.9±7.0%)和Div组(11.2±6.1%)(P&lt；0.05)。UC组浆膜下比例(50.1±15.0%)显著高于CD组(16.9±6.2%)和Div组(12.6±5.7%)。在连续切片的基础上，对卵圆形细胞进行抗CD68染色，对梭形细胞进行抗波形蛋白染色。结论：本研究提示骨桥蛋白可能通过增强免疫活性在UC发病机制中发挥重要作用。较少

项目成果

Hideki Masuda: "Distinct Gene Expression of Osteopontin in Patients with Ulcerative Colitis"Journal of Surgical Research. 111.1. 85-90 (2003)

Hideki Masuda：“溃疡性结肠炎患者骨桥蛋白的独特基因表达”外科研究杂志。

Distinct Gene Expression of Osteopontin in Patients with Ulcerative Colitis

溃疡性结肠炎患者骨桥蛋白的独特基因表达

• 发表时间: 2003
• 期刊: Journal of Surgical Research 111
• 作者: Hideki Masuda;Yasuo Takahashi;Satoshi Asai;Tadatoshi Takayama
• 通讯作者: Tadatoshi Takayama

Osteopontin expression in ulcerative colitis is distinctly different From Crohn's disease and diverticulitis

溃疡性结肠炎中骨桥蛋白的表达与克罗恩病和憩室炎明显不同

• 发表时间: 2005
• 期刊: Journal of Gastroenterology 40・4(In press)
• 作者: Iwaya T;Maesawa C;Kimura T;Ogasawara S;Ikeda K;Kimura Y;Noda Y;Ishida K;Sato N;Saito K;Masuda T.;Hideki Masuda et al.
• 通讯作者: Hideki Masuda et al.

Osteopontin expression in ulcerative colitis is distinctly different from that in Crohn' s disease and diverticulitis.

溃疡性结肠炎中的骨桥蛋白表达与克罗恩病和憩室炎中的骨桥蛋白表达明显不同。

• 发表时间: 2005
• 期刊: J Gastroenterol 40・4
• 作者: Harada C;Harada T;Mitamura Y;Quah AH-M;Ohtsuka K;Kotake S;Ohno S;Wada K;Takeuchi S;Tanaka K;桑名正隆;Watanabe H;Yamakawa K.;Masuda H
• 通讯作者: Masuda H