Research on the assesment of the effect of chemotherapy in malignant gliomas using genome DNA micioanay

基因组DNA微分析评估恶性胶质瘤化疗效果的研究

• 批准号: 15591542
• 负责人: ARAI Hiroshi
• 金额: $ 2.3万
• 依托单位: Iwate Medical Univeisity
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591542/
• 关键词: Malignant glioma; Genome DNA microarray; Chemotherapy; 補充療法

Glioblastoma is a rapidly growing tumor that accounts for more than 50% of all primary gliomas. Amplification of oncogenes and deletion of tumor suppressor genes frequently affects tumor progression. Thus, the goal of this study was to conduct a comprehensive analysis of gene aberrations of individual glioblastomas. A genome DNA microarray (GenoSensor Array^<TM> 300), spotted with 287 target genes, was used to analyze resected tissue from 11 different high-grade gliomas. The average number of gene aberrations was 9.0 per case (WHO grade III) and 13.3 per case (WHO grade IV). EGFR was the most frequent amplified gene in this series (4/11 cases), and high-level amplification was also detected for EGFR, SAS/CDK4 and AKT1. Deleted genes with high frequency were observed in 6 of 11 cases (54.5%) and included FGFR2, MTAP and DMBT1. The detected gene aberrations were matched to the classical primary glioblastoma pathway in 5 of 9 cases. We conclude that the GenoSensor Array^<TM> 300 genomic DNA microarray is a useful method for the comprehensive identification of amplified and deleted genes in glioblasto

胶质母细胞瘤是一种快速生长的肿瘤，占所有原发性胶质瘤的50%以上。癌基因的扩增和抑癌基因的缺失经常影响肿瘤的进展。因此，本研究的目的是对单个胶质母细胞瘤的基因畸变进行全面分析。使用基因组DNA微阵列（GenoSensor Array^<TM>300），点样287个靶基因，分析11个不同高级别胶质瘤的切除组织。基因畸变的平均数为9.0/例（WHO III级）和13.3/例（WHO IV级）。EGFR是该系列中最常见的扩增基因（4/11例），EGFR、SAS/CDK 4和AKT 1也检测到高水平扩增。在11例中有6例（54.5%）观察到高频率的EGFR基因，包括FGFR 2、MTAP和DMBT 1。9例中有5例的基因畸变与经典的原发性胶质母细胞瘤通路相匹配。我们认为GenoSensorArray^300<TM>基因组DNA微阵列是一种综合鉴定胶质母细胞中扩增和缺失基因的有效方法

项目成果

ラトケ嚢胞に伴う下垂体柄部肉芽腫性病変の一手術例

拉特克囊肿相关垂体柄肉芽肿性病变手术一例

• 发表时间: 2003
• 期刊: Neurological Surgery 31(11)
• 作者: 荒井 啓史;佐々木 輝夫;荒井 啓史;Hiroshi Arai;Hiroshi Arai;Teruo Sasaki;Takaaki Beppu;Takaaki Beppu;別府 高明;佐々木 輝夫;荒井 啓史;小守林 靖一
• 通讯作者: 小守林 靖一

Measurement of fractional anisotropy using diffusion tensor MRI in supratentoriala strocytic tumpors

使用弥散张量 MRI 测量幕上星形细胞肿瘤的分数各向异性

• 发表时间: 2003
• 期刊: Journal of Neuro-Oncology 63(29)
• 作者: 荒井 啓史;佐々木 輝夫;荒井 啓史;Hiroshi Arai;Hiroshi Arai;Teruo Sasaki;Takaaki Beppu;Takaaki Beppu;別府 高明;佐々木 輝夫;荒井 啓史;小守林 靖一;小林 哲人;Takaaki Beppu;Takaaki Beppu
• 通讯作者: Takaaki Beppu

神経膠腫におけるconnective tissue growth factor (CTGF)の発見とその血管新生への関与

神经胶质瘤中结缔组织生长因子（CTGF）的发现及其参与血管生成

• 发表时间: 2003
• 期刊: 岩手医学会雑誌 55(2)
• 作者: 荒井 啓史;佐々木 輝夫;荒井 啓史;Hiroshi Arai;Hiroshi Arai;Teruo Sasaki;Takaaki Beppu;Takaaki Beppu;別府 高明;佐々木 輝夫;荒井 啓史;小守林 靖一;小林 哲人
• 通讯作者: 小林 哲人

Measurement of fractional anisotropy using diffusion tensor MRI in supratentorial astrocytic tumors

• DOI: 10.1023/a:1023977520909
• 发表时间: 2003-06-01
• 期刊: JOURNAL OF NEURO-ONCOLOGY
• 影响因子: 3.9
• 作者: Beppu, T;Inoue, T;Kabasawa, H
• 通讯作者: Kabasawa, H

Detection of gene amplification and deletion in high-grade gliomas using a genome DNA microarray (GenoSensor Array 300)

使用基因组 DNA 微阵列 (GenoSensor Array 300) 检测高级神经胶质瘤中的基因扩增和缺失

• 发表时间: 2003
• 期刊: Brain Tumor Pathology 20
• 作者: 荒井 啓史;佐々木 輝夫;荒井 啓史;Hiroshi Arai;Hiroshi Arai;Teruo Sasaki
• 通讯作者: Teruo Sasaki