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Voiding function in mice lacking TRPV 1

缺乏 TRPV 1 的小鼠的排尿功能

基本信息

批准号：
15591675
负责人：
NAKAMURA Yasuo
金额：
$ 2.24万
依托单位：
Akita University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591675/
关键词：
bladder capsaicin knockout mice TRPV 1

项目摘要

Purpose :Some investivators reported that transient receptor potential vanilloid 1 (TRPV1) played an important role during bladder inflammation, namely in hyperalgesia and hyperactivity. However, the mechanisms of activation during chronic bladder inflammation are poorly understood. Therefore, we evaluated the bladder function in mice lacking TRPV1 (TRPV1 KO) with cyclophosphamide (CYP)-induced cystitis.Methods :1.Cystometryin conscious restrained mice. TRPV1 KO and wild type control (WT) were administered CYP (150 mg/kg) or vehicle (sterile water) intraperitoneally. Forty-five hours after administration of CYP, the mice were anesthetized with sevoflurane for surgical insertion of an intravesical catheter. After the surgery, cystometry was performed in conscious restrained mice by infusing saline into the bladder at a constant rate (0.5 ml/hr).2.Histopathological examination. TRPV1 and WT were administered CYP (150 mg/kg) or vehicle intraperitoneally. Forty-eight hours after administra … More tion of CYP, the mice were anesthetized. Bladder was taken intact and fixed for 24 hours in 10% buffered formalin. Tissue (3 micrometer thickness) was stained with hematoxylin-eosin. Histological damage, including hemorrhage and inflammation were evaluated.Results :1.Cystometry in conscious restrained mice. In vehicle administrated mice, there was no significant difference in intercontraction interval (ICI) between WT and TRPV1 KO. In WT, ICI of CYP administrated group was significantly shorter than that of vehicle administrated group. In TRPV1 KO, there was no significant difference in ICI between vehicle administrated group and CYP administrated group. There was no significant difference in maximal voiding pressure between each group.2.Histopathological examination. Vehicle administrated mice had histologically normal bladders. CYP administration caused hemorrhage and inflammation of bladders in both TRPV1 KO and WT. Under microscopic examination, the area and size of inflammation in the bladder were similar in TRPV1 and WT.Conclusions :TRPV1 might be responsible for mechanisms of bladder hyperactivity in mice with CYP-induced cystitis. Less

目的：一些研究者报道瞬时受体电位香草素1（TRPV 1）在膀胱炎症过程中，即在痛觉过敏和活动过度中发挥重要作用。然而，在慢性膀胱炎症过程中的激活机制知之甚少。因此，我们研究了TRPV 1基因敲除小鼠（TRPV 1 KO）的膀胱功能。TRPV 1 KO和野生型对照（WT）经腹膜内给药三次（150 mg/kg）或媒介物（无菌水）。给药后45小时，将小鼠用七氟烷麻醉，用于膀胱内导管的手术插入。手术结束后，通过以恒定速率（0.5ml/hr）将生理盐水灌注到清醒的束缚小鼠的膀胱中来进行膀胱压力测量。TRPV 1和WT腹膜内给药，剂量为150 mg/kg或溶媒。给药后48小时 ...更多信息注射后，将小鼠麻醉。完整取出膀胱，并在10%缓冲福尔马林中固定24小时。用苏木精-伊红对组织（3微米厚）进行染色。结果：1.清醒束缚小鼠膀胱测压。在给予溶剂的小鼠中，WT和TRPV 1 KO之间的收缩间期（ICI）无显著差异。在WT中，给药组的ICI显著短于给药组。在TRPV 1 KO中，溶媒给药组和顺铂给药组之间的ICI无显著差异。各组最大排尿压差异无统计学意义。给予溶媒的小鼠具有组织学正常的膀胱。在TRPV 1 KO和WT中，给药后引起膀胱出血和炎症。在显微镜下，在膀胱炎症的面积和大小是相似的TRPV 1和WT。结论：TRPV 1可能是CYP诱导的膀胱炎小鼠膀胱过度活动的机制。少