Role of CRP-positive cerebellar climbing fiber system on the development of vestibular compensation : An animal model study.

CRP 阳性小脑攀爬纤维系统对前庭代偿发展的作用：动物模型研究。

• 批准号: 15591817
• 负责人: TAKEDA Noriaki
• 金额: $ 1.92万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591817/
• 关键词: cerecellar; animal model; corticotropin-releasing factor; vestibular cerebellum; unipolar brush cell; mossy fiber; climbing fiber; vestibular compensation

Role of CRP-positive cerebellar climbing fiber system in the development of vestibular compensationThe present immunohistochemical study demonstrated an increase in number of corticotropin-releasing factors (CRF)-positive mossy and climbing fiber terminals in the vestibulocerebellum in an ataxic mutant, rolling mouse Nagoya (RMN). Unipolar brush cells (UBCs), a class of interneurons in the vestibulocerebellum, amplifies excitatory inputs from mossy fibers. The number of calretinin (CR)-positive UBCs in the vestibulocerebellum was no different between RNM and the control mice. But, the number of CFR-positive mossy fiber terminals in closely apposition to CR-positive UBCs was 5-fold greater in RMN than in the controls. It is suggested that CRF-positive mossy fiber terminals in contact with UBCs in the vestibulocerebellum compensate the cerebellar ataxia in RMN.An attempt was made to clarify the roles of CRF-positive mossy fiber terminals in contact with UBCs in the vestibulocerebellum in the development of vestibular compensation. After unilateral labyrinthectomy in mice, there were no changes in number of CRF-positive mossy fiber terminals in contact with UBCs in the vestibulocerebellum. Although the number of CRF-positive climbing fiber terminals was increased in the vestibulocerebellum of RMN, there were no changes in number of CRF-positive climbing fiber terminals in the vestibulocerebellum after unilateral labyrinthectomy in mice. All these findings suggest that CRF-positive mossy and climbing fibers in the vestibulocerebellum do not play roles in the development of vestibular compensation.On the contrary, the number of CRF-positive terminals in the ipsilateral medial vestibular nucleus was decreased after unilateral labyrinthectomy in mice, suggesting that CRF affects the development of vestibular compensation in the vestibular nuclei, but not in the vestibulocerebellum.

免疫组织化学研究表明，在共济失调突变型滚动小鼠名古屋（RMN）的前庭小脑中，促肾上腺皮质激素释放因子（CRF）阳性苔藓纤维和爬行纤维终末的数量增加。单极刷状细胞（UBC）是前庭小脑中的一类中间神经元，放大来自苔藓纤维的兴奋性输入。RNM组和对照组小鼠前庭小脑中钙视网膜蛋白（CR）阳性UBC的数量没有差异。但是，与CR阳性UBC紧密贴壁的CFR阳性苔藓纤维末端的数量在RMN中比对照组多5倍。本研究提示，与前庭小脑UBC接触的CRF阳性苔藓纤维终末可能对RMN的小脑共济失调有代偿作用，并试图阐明与前庭小脑UBC接触的CRF阳性苔藓纤维终末在前庭代偿发展中的作用。小鼠单侧小脑切除后，与前庭小脑UBC接触的CRF阳性苔藓纤维终末的数量没有变化。虽然RMN的前庭小脑中CRF阳性攀爬纤维终末的数量增加，但在小鼠单侧小脑切除后，前庭小脑中CRF阳性攀爬纤维终末的数量没有变化。这些结果提示，前庭小脑中CRF阳性苔藓纤维和爬行纤维在前庭代偿的发育中不起作用，相反，单侧前庭神经节切除后，同侧前庭内侧核中CRF阳性终末的数量减少，提示CRF影响前庭神经核中前庭代偿的发育，而不影响前庭小脑。

项目成果

Abnornal expression of tyrosine hydroxylase not accompanied by phosphorylation at serine 40 in cerebellar Purkinje cells of ataxic mutant mice, rolling mouse Nagoya and dilute-lethal

共济失调突变小鼠、滚动小鼠Nagoya和稀致死小鼠小脑浦肯野细胞酪氨酸羟化酶异常表达，不伴有丝氨酸40磷酸化

• 发表时间: 2004
• 期刊: Congenital Anomalies 44
• 作者: K.Sawada;M.Ando;H.Sakata-haga;X.Z.Sun;Y.G.Jeong;S.Hisano;N.Takeda;Y.Fukui;Kazuhiko Sawada
• 通讯作者: Kazuhiko Sawada

Abnormal expression of tyrosine hydroxylase not accompanied by phosphorylation at serine 40 in cerebellar Purkinje cells of ataxic mutant mice, rolling mouse Nagoya and dilute-lethal

共济失调突变小鼠、滚动小鼠Nagoya和稀致死小脑浦肯野细胞酪氨酸羟化酶异常表达，不伴有丝氨酸40磷酸化

• 发表时间: 2004
• 期刊: Congenital Anomalies 44
• 作者: Sawada;K.;Ando;M.;Sakata-Haga;H.;Sun;X-Z.;Jeong;Y.-G.;Hisano;S.;Takeda;N.;Fukui;Y.
• 通讯作者: Y.

Abnornal expression of tyrosine hydroxylase not accompanied by phosphorylation at serine 40 in cerebellar Purkinje cells of ataxic mutant mice, rollimg mouse Nagoya and dilute-lethal

共济失调突变小鼠、rollimg小鼠Nagoya和稀致死小鼠小脑浦肯野细胞酪氨酸羟化酶异常表达，不伴有丝氨酸40磷酸化

• 发表时间: 2004
• 期刊: Congenital Anomalies 44
• 作者: Kazuhiko Sawada

安藤正裕, 澤田和彦, 武田憲昭, 他: "ローリングマウス小脳におけるカルレチニン陽性unipolar brush cellにおけるCRF苔状線維終末"頭頸部自律神経. 18. 53-55 (2004)

Masahiro Ando、Kazuhiko Sawada、Noriaki Takeda 等：“滚动小鼠小脑中钙视网膜蛋白阳性单极刷状细胞中的 CRF 苔藓纤维末端”头颈自主神经学。