Functional analysis of protein phosphatase in vestibulo-cereberum induced by unitateral labyrinthectomy in rats

采用差异显示技术鉴定单侧迷路切除(UL)后小脑小叶基因表达的变化。提取假手术大鼠和迷路切除大鼠小脑小叶总RNA，用随机引物组进行聚合酶链式反应(PCR)扩增，用聚丙烯酰胺凝胶电泳法分离产物。上调产物之一是大鼠蛋白磷酸酶2Aβ催化亚单位mRNA，下调产物之一是大鼠谷氨酸受体Delta-2亚单位mRNA。原位杂交免疫组化研究表明，这些分子定位于浦肯野细胞层。迷路切除大鼠UL诱导的眼球震颤在UL后3d内逐渐消失。这些结果表明，UL参与前庭代偿后，这些分子在蒲肯野细胞中的表达发生了变化。

The differential desplay method was used to identify the gene expression which was altered in the cerebellar flocculus after unilateral labyrintyectomy (UL).Total RNA from flocculi of sham-operated and labyrinthectomized rats was isolated, amplified by PCR using arbitrary primer sets and separated by electrophoresis on a polyacrylarmide gel.PCR products, of which amounts were significantly different in samples from labyrinthectomized rats and those from controls, were cut out of the gel and sequenced.One of the up-regulated products was the rat protein phosphatase 2A beta catalytic subunit mRNA and one of the down-regulated products was the rat glutamate receptor delta-2 subunit mRNA.Ilistochemical study of in situ hybridization showed that those molecules were localized in the Purkinje cell layer.In labyrinthectomized rats, UL-induced nystagmus gradually disappeared within 3days after UL.These findings suggested that changes in expression of those molecules in the floccular Purkinje cells after UL is involved in vestibular compensation.