Development of with biological molecules immobilized titanium using tresyl chloride technique

我们的目标是将细胞粘附蛋白固定在钛上，以改善骨和组织的反应。对钛表面进行三酰氯处理可以有效地将细胞粘附蛋白与钛结合。三烷基氯活化钛表面的碱性氢氧根。钛表面氧化条件的不同对细胞粘附蛋白在钛上的固定没有影响。通过x射线光电子能谱或傅里叶变换红外光谱的测量，证实了三酰氯对钛表面碱性OH的活化作用和细胞粘附蛋白在钛表面的固定作用。用石英晶体微天平技术测定钛表面的结合蛋白，发现用三酰氯活化技术可以将更多的蛋白固定在钛表面。半经验分子轨道计算表明，三酰氯活化影响前沿分子轨道能级。利用氯化三烷基活化技术将细胞粘附蛋白固定在钛上，增强了人牙龈成纤维细胞对钛的初始附着。综上所述，三酰氯活化技术是一种非常有用的细胞粘附蛋白在钛上的固定化技术。

We aimed to immobilize the cell-adhesive proteins onto titanium for improving the bone and tissue response. Tresyl chloride treatment for titanium surface was effective for binding the cell-adhesive proteins onto titanium. Tresyl chloride activated the basic OH of titanium surface. The difference of oxidation conditions of titanium surfaces did not provide any influences for the immobilization of cell-adhesive protein onto titanium. The activation of basic OH of titanium surface by tresyl chloride treatment and the immobilization of cell-adhesive proteins onto titanium were confirmed by the measurement of X-ray photoelectron spectroscopy or Fourier-transform infrared spectroscopy. The measurement of binding proteins onto titanium using quartz-crystal microbalance technique revealed that greater amount of proteins could be immobilized onto titanium surface using tresyl chloride-activation technique. Semi-empirical molecular orbital calculation suggested that the tresyl chloride activation influenced the energy levels of frontier molecular orbital. The initial cell attachment of human gingival flbroblast to titanium was enhanced by the immobilization of cell-adhesive proteins onto titanium using tresyl chloride-activation technique.In conclusion, tresyl chloride-activation technique was very useful technique for the immobilization of cell-adhesive proteins onto titanium.