Application of three-dimensional scaffolds for regenerative medicine with the use of molecular precursor

分子前体三维支架在再生医学中的应用

• 批准号: 17592046
• 负责人: HAYAKAWA Tohru
• 金额: $ 2.24万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592046/
• 关键词: three-dimensional scaffold; regenerative medicine; titanium; titanium fiber mesh; molecular precursor method; thin apatite film; carbonate-containing apatite; X-ray photoelectron spectroscopy; チタンファーバーメッシュ; 骨適合性

In the present study, the influence of thin carbonate-containing apatite (CA) coating on trabecular bone response to cylindrical titanium fiber mesh (porosity of 85 %, pore size of 200-300 μm, 2.8mm diameter x 6 mm length) implants was investigated. Thin CA coatings were deposited by the so-called molecular precursor method. Molecular precursor solution was obtained by adding dibutylammonium diphosphate salt to Ca-EDTA/ amine ethanol solution by adjusting Ca/P=1.67. Sintered cylindrical titanium fiber mesh was immersed into molecular precursor solution and then fired at 600 ℃ for 2 hr. The immersion and firing process was repeated three times. Electron probe microanalysis (EPMA) confirmed that an adherent thin CA film could be deposited on the inside of titanium fiber mesh. After the immersion of a CA-coated titanium fiber mesh in simulated body fluid, apatite crystals precipitated on the titanium fiber mesh. Uncoated and CA-coated titanium fiber mesh was inserted into the trabecular bone of the left and right femoral condyles of rabbits. Histological and histomorphometrical evaluation revealed a significantly greater amount of bone formation inside the porous area of the CA-coated titanium fiber mesh after 12 weeks of implantation. The present results suggested that a thin CA-coated titanium mesh has better osteoconductivity and will be useful for a three-dimensional scaffold.

本研究研究了含碳酸盐磷灰石（CA）薄涂层对柱状钛纤维网（孔隙率85%，孔径200-300 μm，直径2.8mm x长度6 mm）种植体骨小梁响应的影响。通过所谓的分子前驱体法沉积了薄的CA涂层。通过调节Ca/P=1.67，在Ca- edta /胺乙醇溶液中加入二磷酸二丁铵，得到分子前体溶液。将烧结好的圆柱形钛纤维网浸入分子前驱体溶液中，在600℃下焙烧2小时。浸渍烧成过程重复三次。电子探针显微分析（EPMA）证实，在钛纤维网内部可以沉积一层粘附的CA薄膜。将涂有ca的钛纤维网浸入模拟体液后，磷灰石晶体在钛纤维网上沉淀。将未涂布和ca涂布的钛纤维网分别插入兔左右股骨髁的小梁骨。组织学和组织形态学评估显示，植入12周后，ca涂层钛纤维网多孔区域内的骨形成量明显增加。目前的研究结果表明，薄的ca涂层钛网具有更好的骨导电性，将用于三维支架。

项目成果

Gene expression of MC3T3-E1 cells on fibronectin-immobilized titanium using tresyl chloride activation technique

• DOI: 10.4012/dmj.26.647
• 发表时间: 2007-09-01
• 期刊: DENTAL MATERIALS JOURNAL
• 影响因子: 2.5
• 作者: Pugdee, Kamolparn;Shibata, Yasuko;Hayakawa, Tohru
• 通讯作者: Hayakawa, Tohru

Fibronectin adsorption onto plasma-polymerized hexamethyldisiloxane coating

纤连蛋白吸附在等离子体聚合的六甲基二硅氧烷涂层上

• 发表时间: 2006
• 期刊: Journal of Oral tissue Engineering 3・3
• 作者: 足立正徳;若松宣一;亀水秀男;飯島まゆみ;土井豊;Tohru Hayakawa
• 通讯作者: Tohru Hayakawa

Atomic force microscopic images of acidic conditioning agents on various regions of human dentin

酸性调理剂对人牙本质不同区域的原子力显微镜图像

• 发表时间: 2006
• 期刊: Journal of Oral tissue Engineering 3・3
• 作者: 足立正徳;若松宣一;亀水秀男;飯島まゆみ;土井豊;Hiroyuki Mishima
• 通讯作者: Hiroyuki Mishima

Molecular precursor method can coat thin carbonate-containing apatite (CA) films inside titanium fiber mesh

分子前驱体方法可以在钛纤维网内涂覆薄薄的含碳酸盐磷灰石（CA）薄膜

• 发表时间: 2005
• 期刊: Journal of Oral Tissue Engineering 3・1
• 作者: Saitoh;S.;Araki;Y.;Taira;M.;Tohru Hayakawa
• 通讯作者: Tohru Hayakawa

Thin carbonate apatite layer deposited on titanium using molecular precursor method

使用分子前驱体方法在钛上沉积薄碳酸盐磷灰石层

• 发表时间: 2006
• 期刊: Journal of Oral tissue Engineering 3・3
• 作者: Hagiwara Y;Nakajima K;Tsuge T;McGlumphy EA.;Tohru Hayakawa
• 通讯作者: Tohru Hayakawa