Immobilization of cell adhesive protein on dental implant

细胞粘附蛋白在牙种植体上的固定化

• 批准号: 12671904
• 负责人: HAYAKAWA Tohru
• 金额: $ 2.18万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671904/
• 关键词: dental implant; titanium implant; cell-adhesive protein; epithelial attachment; tresyl chloride; fibronectin; XPS; surface treatment; 歯科用インプラント; 細胞接着因子; 固定化; チタン; ハイドロキシアパタイト

The aim of this study is to bound fibronectin directly on tresyl chloride (2, 2, 2 -trifluoroethanesulfonyl chloride) treated titanium surface for the development of tight connection of dental implant to gingival and/or epithelial tissue. Basic terminal OH groups of mirror polished titanium were reacted with tresyl chloride at 37 ℃ for 2 days. Tresylated titanium disk was immersed into the fibronectin/PBS solution for 24 h at 37 ℃.The activation reaction of basic OH of titanium with tresyl chloride was confirmed by F1s and O1s spectra in the measurement of X-ray photoelectron spectroscopy (XPS) and -O-S-O_2 bonds in the measurement of the Fourier transform infrared Reflection-Absorption spectrometer (FT-IR-Ras). XPS spectra after the reaction of fibronectin with titanium demonstrated the remarkable effect of the activation of terminal OH groups with tresyl chloride treatment. N1s peak derived from attached fibronectin was still remaining after 60 s argon-ion etching on the tresylated titanium. On the contrary N1s peak of the specimen without tresyl chloride treatment was almost disappeared after only 10 s argon-ion etching. Direct attachment of fibronectin to tresylated titanium was not removed after 60 s ultrasonic cleaning.Fibronectin, well-known cell-adhesive proteins, could be easily attached on thetitanium surface using tresyl chloride activation technique.

本研究的目的是将纤维连接蛋白直接结合在三酰氯（2,2,2 -三氟乙烷磺酰氯）处理过的钛表面，以促进种植牙与牙龈和/或上皮组织的紧密连接。镜面抛光后的钛基端羟基与三酰氯在37℃下反应2天。Tresylated钛盘在纤维连接蛋白/PBS溶液中浸泡24 h，温度37℃。通过x射线光电子能谱（XPS）测量中的F1s和O1s光谱以及傅里叶变换红外反射吸收光谱仪（FT-IR-Ras）测量中的-O-S-O_2键，证实了钛与三酰氯的碱性OH活化反应。纤维连接蛋白与钛反应后的XPS光谱表明，三酰氯处理对末端OH基团的活化效果显著。在三烷基化钛上氩离子刻蚀60 s后，附着的纤维连接蛋白产生的N1s峰仍然存在。而未经三酰氯处理的样品，经过10 s氩离子刻蚀后，N1s峰几乎消失。超声清洗60 s后，纤维连接蛋白与三烷基化钛的直接附着未被去除。纤维连接蛋白是一种众所周知的细胞粘附蛋白，利用三酰氯活化技术可以很容易地附着在钛表面。

项目成果

谷本安浩, 安田清次郎, 早川 徹, 根本君也: "ハイドロキシアパタイトコーティングインプラントの有限要素方による応力解析-アパタイト層厚さおよび弾性率の影響-"日本口腔インプラント学会誌. 14・3. 414-422 (2001)

Yasuhiro Tanimoto、Seijiro Yasuda、Toru Hayakawa、Kimiya Nemoto：“通过有限元法对羟基磷灰石涂层种植体进行应力分析 - 磷灰石层厚度和弹性模量的影响 -”日本口腔种植学会杂志 14・3。 (2001)

Tohru Hayakawa, Masao Yoshinari, Toshirou Sakae, Kimiya Nemoto: "Calcium phosphate formation on the phosphorylated dental bonding agent in electrolyte solution"Journal of Oral Rehabilitation. (in press).

Tohru Hayakawa、Masao Yoshinari、Toshirou Sakae、Kimiya Nemoto：“电解质溶液中磷酸化牙科粘合剂上的磷酸钙形成”口腔康复杂志。

Tohru Hayakawa, Masao Yoshinari, Toshirou Sakae, Kimiya Nemoto: "Calcium phosphate deposition on dental bonding agent in electrolyte solution"Proceeding of the International Conference on Dentin/Pulp Complex 2001. (in press).

Tohru Hayakawa、Masao Yoshinari、Toshirou Sakae、Kimiya Nemoto：“电解质溶液中牙科粘合剂上的磷酸钙沉积”2001 年牙本质/牙髓复合物国际会议记录。（正在出版）。

Tohru Hayakawa, Masao Yoshinari, Hideo Kiba et al.: "Trabecular bond response to surface roughened and calcium phosphate (Ca-P) coated titanium implants"Biomaterials. 23・4. 1025-1031 (2002)

Tohru Hayakawa、Masao Yoshinari、Hideo Kiba 等：“对表面粗糙和磷酸钙 (Ca-P) 涂层的钛植入物的小梁粘合反应”23・4 (2002)。

Tohra Hayakawa,Kazuyo KiKutake,Kimiya Nemoto: "Conformational and Quantum Analysis of Dental Adhesive Carboxylic Acid and Carboxylic Acid Anhydride Monomers"Dental Materials Journal. 20(1)(印刷中). (2001)

Tohra Hayakawa、Kazuyo KiKutake、Kimiya Nemoto：“牙科粘合剂羧酸和羧酸酐单体的构象和量子分析”《牙科材料杂志》20(1)（印刷中）。