Investigation of stress response system mediated by HPA-axis in periodontitis

HPA轴介导的牙周炎应激反应系统研究

• 批准号: 15592188
• 负责人: YOSHINO Hiroshi
• 金额: $ 2.18万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592188/
• 关键词: stress; HPA-axis; periodontal tissue; ストレス反応系; HPA axis

Hypothalamic-pituitary-adrenal-axis (HPA-axis) is the main response system for physiological stress. The last productive material of this system is cortisol. It has been reported that cortisol is in saliva and gingival crevicular fluid. And recently, it has been demonstrated that mRNA for HPA-axis components and protein in various peripheral tissues. In this study, we investigated whether there is HPA-axis like system in periodontal tissue or not.(1)mRNA for Corticotropin releasing hormone (CRH), CRH receptor 1 (CRHR1), Proopiomelanocortin (POMC), Prohormone convertase-1 (PC-1), Adrenocorticotropic hormone receptor (ACTHR) and CYP11A1 were demonstrated in culture cells from human periodontal tissue.(2)CRH and ACTH were produced by cultured human gingival fibroblasts and keratinocytes.(3)CRH and ACTH were observed immunohistocemically in periodontal tissue. Positive reaction in tissue from periodontal patients were stronger than from healthy volunteers.These results suggest that there is stress response system at the site of periodontal tissue and this system may work in the periodontitis locally

下丘脑-垂体-肾上腺轴(HPA轴)是机体对生理应激的主要反应系统。这一系统的最后一种生产性物质是皮质醇。据报道，皮质醇存在于唾液和牙周沟液中。近年来，已有研究表明HPA轴成分和蛋白在多种外周组织中均有表达。本研究探讨了牙周组织中是否存在促肾上腺皮质激素释放激素(CRH)、CRH受体1(CRHR1)、前阿片黑素皮质素(POMC)、前激素转换酶-1(PC-1)、促肾上腺皮质激素受体(ACTHR)和Cyp11a1的基因表达。(2)CRH和ACTH由培养的人牙周成纤维细胞和角质形成细胞产生。牙周病患者牙周组织中的阳性反应强于健康志愿者，提示牙周组织部位存在应激反应系统，该系统可能在局部牙周炎中起作用