Investigation of stress response system mediated by HPA-axis in periodontitis

下丘脑-垂体-肾上腺轴（HPA-axis）是生理应激的主要反应系统。这个系统最后产生的物质是皮质醇。据报道，皮质醇存在于唾液和牙龈沟液中。近年来，hpa轴组分和蛋白的mRNA在各种外周组织中均有表达。本研究探讨牙周组织中是否存在hpa轴样系统。(1)人牙周组织培养细胞中促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素受体1 （CRHR1）、促肾上腺皮质激素原（POMC）、激素转化酶原-1 （PC-1）、促肾上腺皮质激素受体（ACTHR）和CYP11A1的mRNA表达。(2)培养人牙龈成纤维细胞和角质形成细胞产生CRH和ACTH。(3)免疫组化观察牙周组织CRH和ACTH。牙周患者组织阳性反应较健康志愿者强。提示牙周组织部位存在应激反应系统，该系统可能在局部牙周炎中起作用

Hypothalamic-pituitary-adrenal-axis (HPA-axis) is the main response system for physiological stress. The last productive material of this system is cortisol. It has been reported that cortisol is in saliva and gingival crevicular fluid. And recently, it has been demonstrated that mRNA for HPA-axis components and protein in various peripheral tissues. In this study, we investigated whether there is HPA-axis like system in periodontal tissue or not.(1)mRNA for Corticotropin releasing hormone (CRH), CRH receptor 1 (CRHR1), Proopiomelanocortin (POMC), Prohormone convertase-1 (PC-1), Adrenocorticotropic hormone receptor (ACTHR) and CYP11A1 were demonstrated in culture cells from human periodontal tissue.(2)CRH and ACTH were produced by cultured human gingival fibroblasts and keratinocytes.(3)CRH and ACTH were observed immunohistocemically in periodontal tissue. Positive reaction in tissue from periodontal patients were stronger than from healthy volunteers.These results suggest that there is stress response system at the site of periodontal tissue and this system may work in the periodontitis locally