Development of endotoxin-detection reagents for periodontal treatments with human lactoferrin

人乳铁蛋白牙周治疗内毒素检测试剂的研制

• 批准号: 15592198
• 负责人: NAKASHIMA Keisuke
• 金额: $ 2.24万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592198/
• 关键词: Lactoferrin; Endotoxin

1.Binding capability to LFS: Inhibitory effects of LPS-IBP binding were measured for lactoferrin-derived peptides on the basis of polymyxin B. Inhibitory effects of human lactofenin, (hLf), hLf33K and hLf33R were 0.2 nM, 0.08 nM and 0.05 nM, respectively. There were significant differences among the effects.2.Effects on the inflammatory cytokine release from THP-1 cells : LPS from E cob was added to THP-1 cell culture with hLf, hIf33K or hLf33R, and TNF-a levels and LDH activity in culture supernatant were measured TN-(x levels and LDH activity were significantly decreased upon addition of hLf, hLf33K or hLf33R, however, there was no significant difference among the inhibitory effects. Cell growth of THP-1 measured by cellular intake of dyes was not affected with any reagents.3.Effects on chemotaxis of THP-1 cells : THP-1 cells were stimulated with LPS from E cob and chemotaxis was determined by Boyden chamber method. Chemotaxis were significantly decreased upon addition of hLf, hLf33K or hLf33R, however, there was no significant difference among the inhibitory effects.4.Selection of detection dye and binding of the dye to hLf We consulted Kiriya Chemical Ca about the binding method of the food dye to hLf. They told that approved food dyes once bound to hLf would be no longer allowed to use for food In addition, we consulted Sigma Genosys Co. about the possibility of binding the dye to hLf-derived peptides during synthesis It seemed to be difficult to synthesize the peptide with the dye. We would explore the dye that binds directly to LPS in future.

1.与LFS的结合能力：基于多粘菌素B测量乳铁蛋白衍生肽的LPS-IBP结合的抑制作用。人乳酚宁（hLf）、hLf 33 K和hLf 33 R的抑制作用分别为0.2 nM、0.08 nM和0.05 nM。2.对THP-1细胞释放炎性细胞因子的影响：将来自大肠杆菌的LPS加入到含有hLf、hLf 33 K或hLf 33 R的THP-1细胞培养物中，并测量培养上清液中TNF-α水平和LDH活性。抑制效果无显著差异。3.对THP-1细胞趋化性的影响：用大肠杆菌LPS刺激THP-1细胞，Boyden小室法测定THP-1细胞的趋化性。加入hLf、hLf 33 K和hLf 33 R后，趋化性显著降低，但抑制效果无显著差异。4.检测染料的选择及染料与hLf的结合我们咨询了KICELINE化学Ca关于食品染料与hLf的结合方法。他们告诉我们，一旦与hLf结合，就不再允许将批准的食用染料用于食品。此外，我们咨询了Sigma Genosys Co.关于在合成过程中将染料与hLf衍生肽结合的可能性。似乎很难用染料合成肽。我们将在未来探索直接结合LPS的染料。

项目成果

ヒトラクトフェリンがヒト単球系細胞のケモタキシスに与える影響

人乳铁蛋白对人单核细胞趋化性的影响

• 发表时间: 2004
• 期刊: 東日本歯学雑誌 23
• 作者: 湯本泰弘;中島啓介;藤原 純;池田雅美;小鷲悠典
• 通讯作者: 小鷲悠典

Distributions of human lactoferrin in periodontal pockets

人乳铁蛋白在牙周袋中的分布

• 发表时间: 2004
• 期刊: The Japanese journal of conservative dentistry 47
• 作者: Fujiwara J;Nakashima K;Sugimura N;Kobayashi T;Kowashi Y
• 通讯作者: Kowashi Y

歯周ポケットにおけるラクトフェリンの分布に関する研究

牙周袋内乳铁蛋白分布的研究

• 发表时间: 2004
• 期刊: 日本歯科保存学雑誌 47
• 作者: 藤原 純;中島啓介;杉村典彦;小林孝雄;小鷲悠典
• 通讯作者: 小鷲悠典

Effects of human lactoferrin on the chemotactic response of human Monocytic cells.

人乳铁蛋白对人单核细胞趋化反应的影响。

• 发表时间: 2004
• 期刊: Higashi Nippon dental journal 23
• 作者: Yumoto Y;Nakashima K;Fujiwara J;Ikeda M;Kowashi Y
• 通讯作者: Kowashi Y