Basic research for applying human lactoferrin to periodontal treatments

人乳铁蛋白应用于牙周治疗的基础研究

• 批准号: 12672039
• 负责人: NAKASHIMA Keisuke
• 金额: $ 2.11万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672039/
• 关键词: Lactoferrin; Supragingival plaque; Subgingival plaque; Endotoxin; 歯根面; 歯肉溝滲出液; 末梢血

1. Human lactoferrin (hLf) in gingival crevicular fluid (GCF) and salivaWe measured hLf levels in 15 GCF samples and 7 saliva samples, showing that hLf levels in GCF were significantly higher than in saliva (mean hLf levels; 226 μg/ml in GCF and 20μg/ml in saliva).2. Distribution of hLf on periodontally-diseased dental rootThe area displaying fluorescence was in agreement with the area with calculus after immunofluorescence staining with rabbit anti-hLf antibody. Powdery samples were prepared from extracted roots with a round bur-equipped electric motor-driven system. The samples were collected and partitioned into three groups on the basis of location: Layers I, II and III (from the root surface to the pulp cavity). Significantly higher hLf levels were detected in Layer I from the area with calculus in comparison to Layer I from the area without calculus. hLf and endotoxin levels decreased from Layer I to Layer III.3. hLf levels in supra- and subgingival dental plaqueWe measured hLf levels in supra- and subgingival dental plaque from 19 subjects, showing that hLf levels in supra- and subgingival dental plaque were not significantly different, while the levels were significantly correlated (0.609, p<0.01).4. Effect of hLf on A. actinomycetemcomitans-infected THP-1 cellsLDH activity, DNA fragmentation and TNF-α production in the infected THP-1 cells were reduced upon addition of hLf. These findings suggested that hLf released by neutrophils is bound to LPS leading to inhibition of the immunobiological effects of LPS to host cells, which results in host protection.

1.龈沟液（GCF）和唾液中的人乳铁蛋白（hLf）我们检测了15份GCF样本和7份唾液样本中的hLf水平，结果显示GCF中的hLf水平显著高于唾液（平均hLf水平：GCF中为226 μg/ml，唾液中为20μg/ml）. hLf在牙周病牙根上的分布兔抗hLf抗体免疫荧光染色后，荧光区与牙结石区一致。粉末状样品制备从提取的根与圆钻装备电动机驱动系统。收集样本并根据位置分为三组：第I层、第II层和第III层（从根面到髓腔）。与来自无结石区域的层I相比，在来自结石区域的层I中检测到显著更高的hLf水平。hLf和内毒素水平从层I至层III.3降低。牙龈上和牙龈下牙菌斑中的hLf水平我们测量了19名受试者的牙龈上和牙龈下牙菌斑中的hLf水平，显示牙龈上和牙龈下牙菌斑中的hLf水平没有显着差异，但水平显着相关（0.609，p<0.01）。4. hLf对A.加入hLf后，感染的THP-1细胞LDH活性、DNA断裂和TNF-α的产生减少。这些结果表明，由中性粒细胞释放的hLf与LPS结合，导致LPS对宿主细胞的免疫生物学效应的抑制，从而导致宿主保护。