A proteomic approach to cellulose-synthesizing membrane protein complexes in the ascidian Ciona intestinalis

我们报告了一个蛋白质印迹系统，以评估海鞘玻璃海鞘纤维素合成膜蛋白复合物。针对与C i-CesA部分相对应的几种合成肽产生了兔多克隆抗体，C i-CesA是C.好吧用亲和柱纯化后，使用的肽和与玻璃海鞘丙酮粉末吸附，无论是非特异性反应性或交叉反应性降低，使单一的带得到的估计分子量的Ci-CesA，只有在微粒体制剂。对于样品的制备，法国新闻工作罚款玻璃海鞘胚胎。

We report a western blotting system to assess cellulose-synthesizing membrane protein complexes in the ascidian Ciona intestinalis. Rabbit polyclonal antibodies were raised against several synthetic peptides corresponding to the parts of Ci-CesA, which is the cellulose synthase of C. intestinalis. After purifications with affinity columns using the peptides and an adsorption with Ciona acetone powder, either non-specific reactivity or cross-reactivity was reduced so that the single band was obtained with the estimated molecular mass of Ci-CesA, only in microsome preparations. For sample preparations, French-press worked fine for Ciona embryos.