A proteomic approach to cellulose-synthesizing membrane protein complexes in the ascidian Ciona intestinalis

我们报告了一种蛋白质印迹系统来评估海鞘中合成纤维素的膜蛋白复合体。用兔抗肠道毛囊线虫纤维素合酶Ci-CesA部分的合成肽，制备了兔多克隆抗体。通过亲和层析柱纯化，再用Ciona丙酮粉吸附，降低了非特异性反应性或交叉反应性，从而获得了与Ci-Cesa估计的分子质量相同的单一条带，仅在微体制剂中。在样品准备方面，法国印刷机对Ciona胚胎的处理效果很好。

We report a western blotting system to assess cellulose-synthesizing membrane protein complexes in the ascidian Ciona intestinalis. Rabbit polyclonal antibodies were raised against several synthetic peptides corresponding to the parts of Ci-CesA, which is the cellulose synthase of C. intestinalis. After purifications with affinity columns using the peptides and an adsorption with Ciona acetone powder, either non-specific reactivity or cross-reactivity was reduced so that the single band was obtained with the estimated molecular mass of Ci-CesA, only in microsome preparations. For sample preparations, French-press worked fine for Ciona embryos.