Study on the formation of two polymorphs of cellulose I in Oikopleura

为了了解纤维素生物合成中结晶过程的分子机制，我们分析了海洋脊索动物Oikopleura dioica中的两种纤维素酶，它们被认为参与了纤维素I的两种多晶型的形成。在评估用编码突变的纤维素酶的mRNA注射的胚胎的阴性结果的情况下，我们挑战使用具有细菌、酵母和昆虫细胞宿主的异源系统表达天然形式。

To understand molecular mechanisms behind crystallization processes in cellulose biosynthesis, we analyzed the two cellulose synthases in the marine chordate Oikopleura dioica, those are proposed to be involved in the formation of two polymorphs of cellulose I. With negative results in assessing the embryos injected with mRNAs encoding mutated cellulose synthases, we challenged to express native forms using heterologous system with bacterial, yeast, and insect cell hosts.