Involvement of transcription factors in early motor neuron cell death in cervical segments of avian embryos
转录因子参与禽胚胎颈段早期运动神经元细胞死亡
基本信息
- 批准号:16500223
- 负责人:
- 金额:$ 2.37万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2004
- 资助国家:日本
- 起止时间:2004 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We previously reported that cell death of cervical motor neurons in early avian embryo occurs only in a subpopulation of motoneurons(MNs) that do not express Lim3, a member of LIM-HD transcription factors. In the present study, to elucidate mechanisms that determine cells to die, we clarified development of subtypes of MNs in the cervical and thoracic spinal cord. We examined correlations between birthdates of MNs and their final locations, detailed migration patterns, and change of expression patterns of LIM-HD transcription factors (Isl1, Isl2 and Lim3) and MNR2 protein in the chick embryo.Detailed analysis of expression patterns of Isl1, Isl2 and Lim3 and MNR2 revealed that these four marker proteins were once expressed by all motoneurons in both cervical and thoracic segments. However, Lim3 expression was lost in subpopulations of MNs by E4. In thoracic segments this Lim3-negative population became MNs in the lateral division of the medial motor column that innervate intercostal muscles, whereas in the cervical spinal cord this population disappeared by E5 because of cell death. Both populations became postmitotic in similar developmental stages and are located in similar regions of the motoneurons column. This consistency suggests that two populations are analogous neuronal groups. Difference of rostrocaudal differentiation of the spinal cord, which is determined by expression of Hox transcription factors, may determine different fates of the two populations.Although we pursued this possibility, we could not obtain decisive results within the period of this research project.
我们以前报道过,在早期鸟类胚胎颈部运动神经元的细胞死亡只发生在运动神经元(MN)的亚群,不表达Lim 3,LIM-HD转录因子的成员。在本研究中,为了阐明决定细胞死亡的机制,我们阐明了颈脊髓和胸脊髓中MN亚型的发展。我们研究了MN的出生日期与其最终位置的相关性,详细的迁移模式,以及LIM-HD转录因子(Isl 1,Isl 2和Lim 3)和MNR 2蛋白在鸡胚中的表达模式的变化,Isl 1,Isl 2和Lim 3和MNR 2的表达模式的详细分析表明,这四种标记蛋白在颈段和胸段的所有运动神经元中都有一次表达。然而,Lim 3的表达在E4的MN亚群中丢失。在胸段这Lim 3-阴性人口成为MN的内侧运动柱,支配肋间肌的外侧部,而在颈脊髓这个人口消失的E5,因为细胞死亡。这两个人口成为有丝分裂后在类似的发展阶段,并位于运动神经元列的类似地区。这种一致性表明,两个群体是类似的神经元群体。Hox转录因子的表达决定了两个种群脊髓的喙尾向分化差异,这可能决定了两个种群的不同命运,尽管我们一直在寻找这种可能性,但在本研究项目期间,我们未能获得决定性的结果。
项目成果
期刊论文数量(23)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Low temperature stimulates α-melanophore-stimulating hormone secretion and inhibits background adaptation in xenopus laevis.
低温会刺激 α-黑素细胞刺激激素的分泌,并抑制非洲爪蟾的背景适应。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:TONOSAKI Y;CRUIJSEN P.M.J.M.;NISHIYAMA K.;YAGINUMA H.;ROUBOS E.W.
- 通讯作者:ROUBOS E.W.
Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase
- DOI:10.1523/jneurosci.0295-04.2004
- 发表时间:2004-04-07
- 期刊:
- 影响因子:5.3
- 作者:Kobayashi, K;Takahashi, M;Kobayashi, K
- 通讯作者:Kobayashi, K
Identification and characterization of a novel member of murine semaphorin family
- DOI:10.1111/j.1365-2443.2005.00877.x
- 发表时间:2005-08
- 期刊:
- 影响因子:2.1
- 作者:M. Taniguchi;T. Masuda;M. Fukaya;Hirotaka Kataoka;M. Mishina;H. Yaginuma;Masahiko Watanabe;Takao Shimizu
- 通讯作者:M. Taniguchi;T. Masuda;M. Fukaya;Hirotaka Kataoka;M. Mishina;H. Yaginuma;Masahiko Watanabe;Takao Shimizu
Low temperature stimulates alpha-melanophore-stimulating hormone secretion and inhibits background adaptation in Xenopus laevis.
低温刺激非洲爪蟾的α-黑素细胞刺激激素分泌并抑制背景适应。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Tonosaki Y;Cruijsen PM;Nishiyama K;Yaginuma H;Roubos EW.
- 通讯作者:Roubos EW.
Ficolin A and ficolin B are expressed in distinct ontogenic patterns and cell types in the mouse
- DOI:10.1016/j.molimm.2004.11.019
- 发表时间:2005-07-01
- 期刊:
- 影响因子:3.6
- 作者:Liu, Y;Endo, Y;Fujita, T
- 通讯作者:Fujita, T
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YAGINUMA Hiroyuki其他文献
Differential expression of the GDNF family receptors RET and GFRα1, 2, and 4 in subsets of motoneurons : a relationship between motoneuron birthdate and reseptor expression
运动神经元亚群中 GDNF 家族受体 RET 和 GFRα1、2 和 4 的差异表达:运动神经元出生日期与受体表达之间的关系
- DOI:
- 发表时间:
2003 - 期刊:
- 影响因子:0
- 作者:
HOMMA Shunsaku;YAGINUMA Hiroyuki;SHARON Vinsant;SEINO Miho;KAWATA Megumi;THOMAS Gould;SHIMADA Takako;KOBAYASHI Nobumi;RONALD W. Oppenheim - 通讯作者:
RONALD W. Oppenheim
YAGINUMA Hiroyuki的其他文献
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{{ truncateString('YAGINUMA Hiroyuki', 18)}}的其他基金
Involvement of Hox genes in subgroup specific motoneuron death
Hox 基因参与亚组特异性运动神经元死亡
- 批准号:
20500311 - 财政年份:2008
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of the mechanisms of cell death that involves a specific subgroup of motoneurons.
分析涉及特定运动神经元亚组的细胞死亡机制。
- 批准号:
18500266 - 财政年份:2006
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
mechanisms of early motor neuron cell death in cervical segments of avian embryos
禽胚胎颈段早期运动神经元细胞死亡的机制
- 批准号:
14580731 - 财政年份:2002
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
EXAMINATION OF MECHANISMS OF EARLY PROGRAMMED MOTONEURON DEATH IN THE DEVELOPING CHICK CERVICAL SPINAL CORD
发育中雏鸡颈脊髓早期程序性运动神经元死亡机制的研究
- 批准号:
10680704 - 财政年份:1998
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
ANALYSIS OF MECHANISMS OF NEURONAL CELL DEATH USING ADENOVIRUS VECTOR SYSTEM
利用腺病毒载体系统分析神经细胞死亡机制
- 批准号:
08680808 - 财政年份:1996
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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