Involvement of transcription factors in early motor neuron cell death in cervical segments of avian embryos

转录因子参与禽胚胎颈段早期运动神经元细胞死亡

• 批准号: 16500223
• 负责人: YAGINUMA Hiroyuki
• 金额: $ 2.37万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500223/
• 关键词: motoneuron; cervical cord; chick embryo; Lim3; programmed cell death; LIM-HD; Homeobox; LIM-HD遺伝子; ホメオボックス遺伝子; Bax; 頚髄運動神経; LIM-HD転写因子; 最終分裂; 運動神経細胞死; ホメオボックス遺伝子(Hox)

We previously reported that cell death of cervical motor neurons in early avian embryo occurs only in a subpopulation of motoneurons(MNs) that do not express Lim3, a member of LIM-HD transcription factors. In the present study, to elucidate mechanisms that determine cells to die, we clarified development of subtypes of MNs in the cervical and thoracic spinal cord. We examined correlations between birthdates of MNs and their final locations, detailed migration patterns, and change of expression patterns of LIM-HD transcription factors (Isl1, Isl2 and Lim3) and MNR2 protein in the chick embryo.Detailed analysis of expression patterns of Isl1, Isl2 and Lim3 and MNR2 revealed that these four marker proteins were once expressed by all motoneurons in both cervical and thoracic segments. However, Lim3 expression was lost in subpopulations of MNs by E4. In thoracic segments this Lim3-negative population became MNs in the lateral division of the medial motor column that innervate intercostal muscles, whereas in the cervical spinal cord this population disappeared by E5 because of cell death. Both populations became postmitotic in similar developmental stages and are located in similar regions of the motoneurons column. This consistency suggests that two populations are analogous neuronal groups. Difference of rostrocaudal differentiation of the spinal cord, which is determined by expression of Hox transcription factors, may determine different fates of the two populations.Although we pursued this possibility, we could not obtain decisive results within the period of this research project.

我们先前曾报道过，早期禽类胚胎中宫颈运动神经元的细胞死亡仅发生在不表达LIM3的运动神经元（MN）的亚群中，这是LIM3，这是LIM-HD转录因子的成员。在本研究中，为了阐明确定细胞死亡的机制，我们澄清了颈椎和胸椎中MN的亚型的发展。我们检查了MN的生日及其最终位置之间的相关性，详细的迁移模式以及Lim-HD转录因子（ISL1，ISL2和LIM3）的表达模式的变化与雏鸡胚胎中的MNR2蛋白。在ISL1，ISL2和Lim3和Lim3和MNR2的表达模式中进行了详细分析。胸部段。但是，E4在MNS的亚群中丢失了LIM3的表达。在胸部段中，这种LIM3阴性人群在支配肋间肌肉的内侧运动柱的外侧分裂中成为MN，而在颈椎脊髓中，由于细胞死亡，E5消失了。这两个种群在类似的发育阶段都成为有丝分裂后的，并且位于运动神经元柱的类似区域。这种一致性表明两个人群是类似的神经元组。脊髓的鼻旁分化的差异是由HOX转录因子的表达决定的，这可能决定了这两个种群的不同命运。尽管我们采取了这种可能性，但我们在本研究项目期间无法获得决定性的结果。

项目成果

Low temperature stimulates α-melanophore-stimulating hormone secretion and inhibits background adaptation in xenopus laevis.

低温会刺激 α-黑素细胞刺激激素的分泌，并抑制非洲爪蟾的背景适应。

• 发表时间: 2004
• 期刊: J.Neuroendocinol 16
• 作者: TONOSAKI Y;CRUIJSEN P.M.J.M.;NISHIYAMA K.;YAGINUMA H.;ROUBOS E.W.
• 通讯作者: ROUBOS E.W.

Low temperature stimulates alpha-melanophore-stimulating hormone secretion and inhibits background adaptation in Xenopus laevis.

低温刺激非洲爪蟾的α-黑素细胞刺激激素分泌并抑制背景适应。

• 发表时间: 2004
• 期刊: J Neuroendocrinol. 16(11)
• 作者: Tonosaki Y;Cruijsen PM;Nishiyama K;Yaginuma H;Roubos EW.
• 通讯作者: Roubos EW.

Distinct susceptibility of developing neurons to death following Bax overeXpression in the chicken embryo.

鸡胚胎中 Bax 过度表达后，发育中的神经元对死亡具有明显的敏感性。

• 发表时间: 2006
• 期刊: Cell Death Differ. 13
• 作者: Sato N;Sakuma C;Sato Y;Gould TW;Oppenheim RW;Yaginuma H
• 通讯作者: Yaginuma H