Molecular and morphological analysis on the mechanism of regulation by steroid synthesis in lymphoid tissues.

淋巴组织类固醇合成调节机制的分子和形态学分析。

• 批准号: 17590170
• 负责人: TSURUO Yoshihiro
• 金额: $ 2.37万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590170/
• 关键词: lymphoid tissues; steroid; metabolizing enzyme; regulation; immunohistochemistry; rats

Concerning the ability of steroid synthesis in the rat abdominal organs, we already reported that the parietal cells in the rat stomach secrete estrogens into the portal vessels. We hypothesized that there is a species difference in the steroid synthesis in the abdominal organs and in mammals including the primates the lymphoid tissues including spleen can synthesize estrogens and the synthesized steroids make some regulations in the lymphoid tissues or through the relation between the lymphoid tissues and the liver. Therefore we examined immunohistochemically the expression of some steroid metabolizing enzymes including aromatase in the lymphoid tissues, which contained thymus, spleen, lymph nodes and Payer's patches of ileum, of rats during postnatal development. We used the following antibodies against aromatase, 5α-reductase type 1, 2, 3α-hydroxysteroid dehydrogenase, 3β-hydroxysteroid dehydrogenase, cholesterol side-chain cleavage enzyme and so on. These steroid metabolizing enzymes were expressed in the lymphocytes, macrophages, dendritic cells, epithelial reticular cells after the early postnatal period onwards. The cellular pattern of the expression was characteristic of each enzyme.

关于大鼠腹部器官合成类固醇的能力，我们已经报道了大鼠胃壁细胞分泌雌激素进入门脉血管。我们推测，在包括灵长类在内的哺乳动物中，包括脾脏在内的淋巴组织可以合成雌激素，并且合成的类固醇激素在淋巴组织中或通过淋巴组织与肝脏之间的联系进行调节。因此，我们用化学发光法检测了大鼠生后发育过程中胸腺、脾脏、淋巴结和回肠Payer结等淋巴组织中包括芳香化酶在内的类固醇代谢酶的表达。我们用芳香化酶、5α-还原酶1型、2，3α-羟基类固醇脱氢酶、3β-羟基类固醇脱氢酶、胆固醇侧链裂解酶等抗体，发现这些类固醇代谢酶在出生后早期开始在淋巴细胞、巨噬细胞、树突状细胞、上皮网状细胞中表达。表达的细胞模式是每种酶的特征。

项目成果

Persistent median arery in the hand : A report with a brief review of the literature.

手上持续存在的中位数：一份对文献进行简要回顾的报告。

• 发表时间: 2006
• 期刊: Anatomical Science International 81
• 作者: Kawakami;K.;Sato M;Tsuruo Y et al.
• 通讯作者: Tsuruo Y et al.

左椎骨動脈の走行異常の一例.

左椎动脉异常走向的一个例子。

• 发表时间: 2006
• 期刊: 和歌山医学 57
• 作者: 上山敬司;他
• 通讯作者: 他

Glucocorticoid-inducible glutamine synthetase in GH cells of chick embryos, Gallus domesticus : Ontogeny of glutamine synthetase, GH, and Pit-1 protein in the pituitary gland.

鸡胚 GH 细胞中糖皮质激素诱导的谷氨酰胺合成酶，鸡内金：垂体中谷氨酰胺合成酶、GH 和 Pit-1 蛋白的个体发育。

• 发表时间: 2006
• 期刊: The Journal of Poultry Science 43(3)
• 作者: Kobayashi;H.;Miyoshi T;Shirasawa N et al.
• 通讯作者: Shirasawa N et al.

Estrogen alters c-Fos response to immobilization stress in the brain of ovariectomized rats

• DOI: 10.1016/j.brainres.2006.02.008
• 发表时间: 2006-04-21
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Ueyama, Takashi;Tanioku, Tadashi;Tsuruo, Yoshihiro
• 通讯作者: Tsuruo, Yoshihiro