权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The physiological significance and the change of the neurosteroid-producing enzyme in the brain under stress conditions.

应激条件下大脑中神经类固醇生成酶的生理意义和变化。

基本信息

批准号：
09680794
负责人：
TSURUO Yoshihiro
金额：
$ 1.34万
依托单位：
Wakayama Medical College (1998)The University of Tokushima (1997)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680794/
关键词：
5alpha-reductase Neurosteroid Stress Rat Brain Immunohistochemistry 5α-リダクターゼ

项目摘要

Steroid 5alpha-reductase is the enzyme that converts a number of steroids with a C-4,5 double bond and C-3 ketone to 5alpha-reduced metabolites, and the type 1 of two isozymes is present in the brain. We investigated immunohistochemically the change of the enzyme expression and the cellular appearance of 5alpha-reductase-containing rat C6 glioma cells by culturing these cells in vitro in stress conditions, that is, the serum-free medium. The expression of 5alpha-reductase was located in the perinuclear region under the serum-supplemented medium, but distributed in the cytoplasmic region under the serum-free medium. The expression of both GFAP and CNPase was the same between the two culture conditions. The cellular form was spinde in the serum-supplemented medium, and became hypertrophic and multipolar in the serum-free medium. However, the expression of 5alpha-reductase mRNA was the same indifferent of the presence of serum. The immunohistochemical change of 5alpha-reductase expression was induced by the change of enzyme localization within the cells, but not by the enzyme quantity.In the adrenal gland, which is thought to be involved in the stress reactions, 5alpha-reductase was located in the fascicular to reticular layer of the gland and the staining intensity of the enzyme increased after the removal of gonads and decreased by the supplementation of testosterone or estradiol. The enzyme was shown to be located by light and electron microsopy in the cytoplasm of Schwann cells and satellite cells in the peripheral nervous system.

类固醇5α-还原酶是一种将具有C-4，5双键和C-3酮的类固醇转化为5α还原代谢物的酶，大脑中存在两种同工酶。我们用免疫组织化学方法研究了含5α-还原酶的大鼠C6胶质瘤细胞在应激条件下，即无血清培养条件下，细胞形态和酶表达的变化。5α-还原酶在有血清培养基下定位于核周区，而在无血清培养基下分布在胞浆内。在两种培养条件下，GFAP和CNPase的表达相同。有血清培养时细胞形态为纺锤形，无血清培养时细胞形态变得肥大，呈多极化。而血清对5α-还原酶基因的表达无明显影响。在参与应激反应的肾上腺中，5α-还原酶位于腺体的束状层至网状层，去势后5α-还原酶染色强度增加，补充睾酮或雌二醇后，5α-还原酶染色强度降低。光镜和电子显微镜观察表明，该酶定位于周围神经系统雪旺细胞和卫星细胞的胞浆中。