Molecular and morphological analysis of RNA helicase that shows an unique expression in the nervous system by utilizing genetically modified animals

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The monoclonal antibody(4F2) that specifically identifies oligodendrocytes was generated, and the molecule recognized by this antibody proved to be Ddx54, a member of RNA helicase. In the transgenic animals with forced expression of Ddx54, myelination appeared to be accelerated. The 4F2-positive cells were observed in the rat central nervous system from early embryonal to adult stages, and they were specifically expressed in oligodendrocytes using primary cultures of neural tissues. Ddx54 bound to 4 types of MBP isoforms, and especially the 21. 5 kDa isoform of MBP was transported into the nucleus, suggesting its involvement in the signal transduction during the process of myelination in oligodendrocytes.