Ontogeny and differentiation of oligodendrocytes in the central nervous system using the antibody recognizing immature oligodendrocytes.

用小鼠单克隆抗体（克隆4F2和12F7）检测了少突胶质细胞的个体发生和分化，该抗体是利用大量同质的阶段特异性未成熟少突胶质细胞作为免疫原在大鼠早期胚胎发育至成年期的前脑和脊髓等中枢神经系统中产生的。在胚胎早期（E9-E10）脑室及邻近脑室区的神经上皮细胞中观察到克隆4F7抗体的免疫反应。它们广泛分布于大脑和脊髓。4f7阳性细胞未被识别未成熟神经元的TUJ1抗体免疫染色，提示它们是产生少突胶质细胞的祖细胞，而不是神经元。随着发育，这些细胞从脑室区延伸到脑膜下的整个脑壁，出生后最好是在白质而不是灰质中。它们的突起在新生儿期被截断，在成人中精细的辐射突起被拉长以包裹轴突。成人4f7阳性细胞用几种低聚胶质标记物标记。在成人和老年人侧脑室背外侧角的室下区观察到它们，表明存在未成熟的少突胶质细胞。另一个克隆（12F7）在出生后才在白质髓磷脂部分显示出免疫反应性。在白质内，髓磷脂未表现出12F7免疫反应性。

The ontogeny and differentiation of oligodendrocytes were examined immunohistochemically with the mouse monoclonal antibodies (clones 4F2 and 12F7) that were generated using a huge amount of homogeneous population of stage-specific immature oligodendrocytes as a immunogen, in the rat central nervous system including the prosencephalon and the spinal cord during the development from the early embryonal to the adult period. The cells immunoreactive for the antibody of clone 4F7 were observed in the neuroepithelium lining the ventricles and the neighboring ventricular zone in the early embryonal days (E9-E10). They were widely distributed through the brain and spinal cord. The 4F7-positive cells were not immunostained with the TUJ1 antibody that recognizes the immature neurons, suggesting that they are the progenitors to generate the oligodendrocytes, but not neurons. With development, these cells were located th roughout the wall of the brain from the ventricular zone beneath the pia mater, and were preferably in the white matter rather than the gray matter after birth. Their processes were arborized in the neonatal period, and in adults fine radiated processes were elongated to envelop the axons. The 4F7-positive cells were labeled with several oligodedroglial markers in adults. They were observed in the subventricular zone at the dorsolateral corner of the lateral ventricle in adults and senility, suggesting the presence of immature oligodendrocytes. Another clone (12F7) showed the immunoreactivity in the myelin part of the white matter only after birth. Within the white matter, all of the myelin did not show the 12F7 immunoreactivity.