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The role of quorum sensing system in Helicobacter pylori infection

群体感应系统在幽门螺杆菌感染中的作用

基本信息

批准号：
17590399
负责人：
OSAKI Takako
金额：
$ 2.3万
依托单位：
Kyorin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590399/
关键词：
Helicobacter pylori Quorum sensing Autoinducer-2 Flagella Motility Flagellin B クオラムセンシング

项目摘要

Quorum sensing (QS) is a cell-to-cell communication system that regulates bacterial phenotypes, including the expression of virulence factor genes. Helicobacter pylori is associated with gastric disorders in humans and some experimental animals, and possesses the luxS/type 2 autoinducer (AI-2) system. We examined the effects of a specific luxS mutation on the characteristics of H. pylori. On 0.3% agar medium, motility of H. pylori HPKY08 (luxS::cat) was significantly lower than that of wild-type H. pylori TK1402. The luxS-complemented strain HPKY21 exhibited motility comparable to that of H. pylori TK1402. It was shown that the luxS/M-2 system plays an important role in H. pylori motility. The luxS mutant exhibited a reduced infection rate relative to the wild-type parent strain TK1402 in a Mongolian gerbil model. At 3 months after oral inoculation, lower numbers of H. pylori were detected by qRT-PCR in luxS mutant-infected gerbils than in TK1402-infected gerbils. On other pathogenesis factor, adherence to gastric epithelial cells, acid tolerance, survival rate in macrophage like-cells, there was no significant differences between wild type and mutant strains. In both strains, 3-5 polar flagella were observed on electron microscopy. In DNA micro-array using mRNA of both strains at logarithmic phase, 53 genes including flagella associated gene in mutant strain which was 1.5 or more fold expression comparing with wild type strain. In stationary phase, lower expression data of flaA and flaB in luxS mutant than that in wild type by qRT-PCR. By 2D-SDS analysis amount of FlaB in luxS mutant strain was lower than that in wild type strain, significantly.

群体感应（Quorum sensing，QS）是一种细胞间通讯系统，调节细菌表型，包括毒力因子基因的表达。幽门螺杆菌与人类和一些实验动物的胃部疾病有关，并具有luxS/2型自诱导物（AI-2）系统。我们研究了一个特定的luxS突变对H.幽门。在0.3%琼脂培养基上，H. pylori HPKY 08（luxS：：cat）的表达显著低于野生型H. pylori TK 1402。补充luxS的菌株HPKY 21表现出与H. pylori TK 1402。结果表明，luxS/M-2体系在H.幽门运动在蒙古长爪沙鼠模型中，luxS突变体相对于野生型亲本菌株TK 1402表现出降低的感染率。口服接种后3个月，H. pylori的qRT-PCR检测结果显示，在luxS muplatin感染的沙鼠中比在TK 1402感染的沙鼠中更易检测到pylori。在其他致病因子上，对胃上皮细胞的粘附性、耐酸性、巨噬细胞样细胞存活率，野生型与突变株之间无显著差异。电镜下观察到两株菌均有3-5根极鞭毛。利用对数生长期两株菌的mRNA在DNA微阵列中，突变株中包括鞭毛相关基因在内的53个基因的表达量是野生型的1.5倍或更多。qRT-PCR结果显示，在稳定期，luxS突变体中flaA和flaB的表达量低于野生型。2D-SDS分析表明，luxS突变株的FlaB含量显著低于野生型菌株。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Long-term infection of mongolian gerbils with Helicobacter pylori:: Microbiological, histopathological, and serological analyses

DOI：
10.1128/cdli.12.2.347-353.2005
发表时间：
2005-02-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
0
作者：
Nakagawa, S;Osaki, T;Kamiya, S
通讯作者：
Kamiya, S

Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Moneolian gerbil model.

luxS 突变影响沙鼠模型胃粘膜中幽门螺杆菌的运动性和感染性。

DOI：
发表时间：
2006
期刊：
Journal of Medical Microbiology 55
影响因子：
0
作者：
T.Osaki;et al.
通讯作者：
et al.

Mutation of luxS affects motility and infectivity of Helicobacter pylon in gastric mucosa of a Mongolian gerbil model.

luxS 突变影响蒙古沙鼠模型胃粘膜中幽门螺杆菌的运动性和感染性。

DOI：
发表时间：
2006
期刊：
J Med Microbiol 55
影响因子：
0
作者：
Osaki T;Hanawa T;Manzoku T;Fukuda M;Kawakami H;Suzuki H;Yamaguchi H;Xu Yen;Taguchi H;Kurata S;Kamiya S
通讯作者：
Kamiya S

Effect of Helicobacter pylon on DNA synthesis of human epithelial cells.

幽门螺杆菌对人上皮细胞 DNA 合成的影响。