Rapid diagnosis of the systemic inflammatory response. syndrome by the transcription factor activation measuring method development concerning the inflammation

快速诊断全身炎症反应。

基本信息

  • 批准号:
    17590486
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2005
  • 资助国家:
    日本
  • 起止时间:
    2005 至 2006
  • 项目状态:
    已结题

项目摘要

The nuclear factor-kappa B (NF-κB) family of transcription factors is known to play an important role in the regulation of the immune system. NF-κB is activated by bacterial and viral antigens, which leads to the production of proinflammatory cytokines and chemokines. The rapid detection of activated NF-κB activation by the systemic inflammatory response syndrome (SIRS) is considered to be crucial for the treatment of patients with septicemia. The aim of the present study was to evaluate the sensitivity of two methods, electrophoretic mobility shift assay (EMSA) and transcription factor enzyme-linked immunoassay (TF-ELISA). TF-ELISA detected 25 ng of recombinant human NF-κB p50 (rhp50) and 5 mg of TNFα-stimulated HeLa nuclear protein, while EMSA detected approximately 100 ng of rhp50 and 10 mg of HeLa nuclear protein. We found that the TF-ELISA was more sensitive than EMSA in detecting NF-κB. However, it was judged that the 3-6 hour measuring time required in TF-ELISA was excessively long for patients with SIRS. Therefore, the development of new analytical methods with improved sensitivity and, measurement time was necessary for the detection and quantification of activated NF-κB protein in the hospital laboratory. Consequently, we have developed a new NF-κB analyzer based on surface plasmon resonance (SPR), which is recognized as one of the most sensitive direct optical detection methods. This method can detect nanomolar concentrations of NF-κB within 15 minutes. At present, we are carrying out clinical trials using this new transcription factor analysis apparatus for SIRS patients.
已知核因子-κB (NF-κB)转录因子家族在免疫系统调节中发挥重要作用。NF-κB被细菌和病毒抗原激活,导致促炎细胞因子和趋化因子的产生。通过全身性炎症反应综合征(SIRS)快速检测活化的NF-κB活化被认为对败血症患者的治疗至关重要。本研究的目的是评估两种方法的敏感性,电泳迁移量转移法(EMSA)和转录因子酶联免疫分析法(TF-ELISA)。TF-ELISA检测到重组人NF-κB p50 (rhp50) 25 ng和tnf α刺激的HeLa核蛋白5 mg, EMSA检测到约100 ng的rhp50和10 mg的HeLa核蛋白。tnf - elisa检测NF-κB的敏感性高于EMSA。但经判断,对于SIRS患者,TF-ELISA所需的3-6小时测量时间过长。因此,在医院实验室对活化NF-κB蛋白进行检测和定量,需要开发灵敏度更高、测量时间更短的新型分析方法。因此,我们开发了一种基于表面等离子体共振(SPR)的新型NF-κB分析仪,这是公认的最灵敏的直接光学检测方法之一。该方法可在15分钟内检测到NF-κB的纳摩尔浓度。目前,我们正在使用这种新的转录因子分析仪器对SIRS患者进行临床试验。

项目成果

期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Single-cell microarray for analyzing cellular response
  • DOI:
    10.1021/ac0515632
  • 发表时间:
    2005-12-15
  • 期刊:
  • 影响因子:
    7.4
  • 作者:
    Yamamura, S;Kishi, H;Muraguchi, A
  • 通讯作者:
    Muraguchi, A
BMP2 prevents apoptosis of N1511 chodrocytic cells through PI3K/Akt-mediated NF-κB activation.
BMP2 通过 PI3K/Akt 介导的 NF-κB 激活防止 N1511 软骨细胞凋亡。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sugimori K;Matsui K;Motomura H;Tokoro T;Wang J;Kimura T;Kitajima I
  • 通讯作者:
    Kitajima I
流れがわかる実践検査マニュアル上・下巻
实用检验手册第1卷和第2卷,帮助您了解流程
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Yamaji K;Abeyama K;Hashiguchi T;Uchimura T;Maruyama I.;et. al.;Torii K et al.;北島 勲
  • 通讯作者:
    北島 勲
BMP2 prevents apoptosis of N1511 chocrocytic cells through PI3K/Akt-mediated NF-κB activation
BMP2 通过 PI3K/Akt 介导的 NF-κB 激活防止 N1511 巧克力细胞凋亡
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sugimori K;Matsui K;Motomura H;Tokoro T;Wang J;Kitajima I
  • 通讯作者:
    Kitajima I
Pitavastatin at low dose activates endothelial nitric oxide synthetase through PI3K-Akt pathway in endothelial cells.
低剂量的匹伐他汀通过内皮细胞中的 PI3K-Akt 途径激活内皮一氧化氮合成酶。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Wang J;Tokoro T;Matsui K;Higa S;Kitajima I
  • 通讯作者:
    Kitajima I
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KITAJIMA Isao其他文献

KITAJIMA Isao的其他文献

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{{ truncateString('KITAJIMA Isao', 18)}}的其他基金

The integrated system with Tm mapping and the FCS-based NF-kB assay for sepsis patients
针对脓毒症患者的 Tm 图谱和基于 FCS 的 NF-kB 检测的集成系统
  • 批准号:
    25670268
  • 财政年份:
    2013
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Molecular pathological mechanisms of the brain development disorder using the chromatin-remodeling molecule ATRX gene knockout mouse
染色质重塑分子ATRX基因敲除小鼠脑发育障碍的分子病理机制
  • 批准号:
    23300147
  • 财政年份:
    2011
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Application to the emergency care by establishment of high-throughput examination systems for transcription factor NF-κB activation
建立转录因子NF-κB激活高通量检测系统在急救中的应用
  • 批准号:
    23659294
  • 财政年份:
    2011
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of the hypersensitive and rapid detection method for NF-κB activation using by Fluorescence Correlation Spectroscopy and its application to the emergency medicine
荧光相关光谱超灵敏快速检测NF-κB活化方法的建立及其在急诊医学中的应用
  • 批准号:
    20590559
  • 财政年份:
    2008
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Memory and learning disorder analysis using mutation mouse for mental retadation related gene ATRX and elucidation of the genetic control abnormality.
使用智力迟缓相关基因 ATRX 突变小鼠进行记忆和学习障碍分析,并阐明遗传控制异常。
  • 批准号:
    15500210
  • 财政年份:
    2003
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular mechanism of constitutive expression of interleukin 8 in the cancer progress and the development of the angiogenesis control therapy
白细胞介素8组成型表达在癌症进展中的分子机制及血管生成控制治疗的发展
  • 批准号:
    13670315
  • 财政年份:
    2001
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular constructions of oligodeoxynucleotides binding to bFGF targeting for angiogenesis
结合 bFGF 靶向血管生成的寡脱氧核苷酸的分子结构
  • 批准号:
    09470172
  • 财政年份:
    1997
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular cloning related to skeletal or muscle atrophy under microgravity
微重力下骨骼或肌肉萎缩相关的分子克隆
  • 批准号:
    08557151
  • 财政年份:
    1996
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Gene therapy for atherosclerosis by inhibition of nuclear transcriptional factor
通过抑制核转录因子进行动脉粥样硬化的基因治疗
  • 批准号:
    07457174
  • 财政年份:
    1995
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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巨噬细胞炎症细胞因子产生的分子调控
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    RGPIN-2018-06053
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炎症细胞因子制瘤素 M 的小分子抑制剂
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