Molecular cloning related to skeletal or muscle atrophy under microgravity

微重力下骨骼或肌肉萎缩相关的分子克隆

基本信息

  • 批准号:
    08557151
  • 负责人:
  • 金额:
    $ 4.29万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

The effect of 14 days of flight on the vertebrae of rapidly growing rats was studied. No significant difference (p>0.05) was observed between flight rats and ground controls with regard to mechanical hardness or bone mineral density. however, histological examination revealed irregular thickening of the endosteal surface of cortical bones in flight rats, whereas it was uniform in the ground controls. The relative area of lamellar bone showed a significant reduction (p<0.001) in the flight rats. These findings suggest that structural disturbances were due to retardation of endosteal modeling and remodeling. Next, the effects of 14 days of space-flight on beta-adrenoceptor (beta-AR), mitochondrial enzyme activities, and fiber type composition were studied in plantaris muscles. The maximum binding capacity of beta-AR (B max) was significantly lower (>29%) after flight, and the recovery was not complete after 9 days in a 1-G environment. The activity of succinate dehydrogenase (SDH) was measured in muscle samples <5 hr after flight and were found to normalize during 9 days of recovery. It is suggested that the space-flight-induced decrease of B max of beta-AR in plantaris was accompanied by a decreased activity of a mitochondrial inner-membrane enzyme, SDH.Finally, the expression of mRNA in paraspinal muscles between space-flight rats and ground controls was compared using mRNA differential display method in order to identify molecules related to muscle atrophy under microgravity. We found that 70 kDa heat-shock protein (HSP70) and its retated gene, TCP-1, were makedly elevated during 14 days of space-flight, while genes involved in mitochondrial metabolism (mitochondrial adenosine nuclease translocator, mitochondrial cytochrome oxidase subunits), aldolase A and myocyte-specific enhancer binding factor (MEF) were significantly decreased. Some new genes related to muscle atrophy were also detected and we studied the biological functions of these molecules.
研究了14天飞行对快速生长大鼠椎骨的影响。在机械硬度和骨密度方面,飞行大鼠和地面对照组之间没有观察到显著差异(p>0.05)。然而,组织学检查显示,飞行大鼠皮质骨的骨内膜表面不规则增厚,而地面对照大鼠的骨内膜表面均匀增厚。飞行大鼠板层骨相对面积明显减小(P<0.001)。这些结果表明,结构紊乱是由于延迟的骨内膜建模和重塑。接下来,研究了14天太空飞行对跖肌中β-肾上腺素受体(β-AR)、线粒体酶活性和纤维类型组成的影响。β-AR的最大结合容量(B max)在飞行后显著降低(>29%),并且在1-G环境中9天后恢复不完全。琥珀酸脱氢酶(SDH)的活性在飞行后<5小时的肌肉样品中进行了测量,并发现在9天的恢复期间恢复正常。提示微重力条件下大鼠跖肌β-AR的B max降低伴随着线粒体内膜酶SDH活性的降低。最后,利用mRNA差异显示技术比较了微重力条件下大鼠和地面对照大鼠脊旁肌mRNA的表达,以筛选与肌萎缩相关的分子。我们发现,在14天的太空飞行期间,70 kDa热休克蛋白(HSP 70)及其相关基因TCP-1明显升高,而参与线粒体代谢的基因(线粒体腺苷核酸酶转运子、线粒体细胞色素氧化酶亚基)、醛缩酶A和肌细胞特异性增强子结合因子(MEF)显着减少。我们还发现了一些与肌萎缩相关的新基因,并对这些分子的生物学功能进行了研究。

项目成果

期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ohira,Y.,et al.: "Spaceflight effects on β-adrenoreceptor and metabolic propeties in rat plantars." J.Appl.Physiol.81(1). 152-155 (1996)
Ohira, Y., 等人:“太空飞行对大鼠足底 β-肾上腺素受体和代谢特性的影响。”J.Appl.Physiol.81(1) (1996)。
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    0
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G.Yamada, K.Sugimura, S.Nakamura, M-O.Yamada, Y.Okazaki, I.Maruyama, I.Kitajima, T.Minami: "Metal comp[osition and histological analysis of the space flight rat bones." Life Science. 60. 635-642 (1997)
G.Yamada、K.Sugimura、S.Nakamura、M-O.Yamada、Y.Okazaki、I.Maruyama、I.Kitajima、T.Minami:“太空飞行大鼠骨骼的金属成分和组织学分析。”
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    0
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Kimiyoshi Arimura Isao Kitajima,et al.: "Antibodies to potassium channel of PC12in serum of Isaacs'syndrome" Muscle & Nurve. 20(3). 299-305 (1997)
Kimiyoshi Arimura Isao Kitajima 等人:“艾萨克斯综合征血清中 PC12 钾通道抗体”肌肉
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
Ikuro Maruyama, Isao Kitajima, et al: "Thrombin activates NF-kB through thrombin receptor" Recent Progress in Blood Coagulation and Fibrinolysis ELSEVIER,Amsterdam,260 (1997)
Ikuro Maruyama、Isao Kitajima 等人:“凝血酶通过凝血酶受体激活 NF-kB”血液凝固和纤维蛋白溶解的最新进展 ELSEVIER,阿姆斯特丹,260 (1997)
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    0
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J.Nishi, S.Kanekura, S.Takei, I.Kitajima, T.Nakajima, M.R.Wahid, K.Matsuda, M.Yoshinaga, I.Maruyama, K.Miyata: "B cell epitope mapping of the bacterial superantigen staphylococcal enterotoxin B : The Diminant epitope region recognized by intravenous IgG."
J.Nishi、S.Kanekura、S.Takei、I.Kitajima、T.Nakajima、M.R.Wahid、K.Matsuda、M.Yoshinaga、I.Maruyama、K.Miyata:“细菌超抗原葡萄球菌肠毒素的 B 细胞表位作图
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KITAJIMA Isao其他文献

KITAJIMA Isao的其他文献

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{{ truncateString('KITAJIMA Isao', 18)}}的其他基金

The integrated system with Tm mapping and the FCS-based NF-kB assay for sepsis patients
针对脓毒症患者的 Tm 图谱和基于 FCS 的 NF-kB 检测的集成系统
  • 批准号:
    25670268
  • 财政年份:
    2013
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Molecular pathological mechanisms of the brain development disorder using the chromatin-remodeling molecule ATRX gene knockout mouse
染色质重塑分子ATRX基因敲除小鼠脑发育障碍的分子病理机制
  • 批准号:
    23300147
  • 财政年份:
    2011
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Application to the emergency care by establishment of high-throughput examination systems for transcription factor NF-κB activation
建立转录因子NF-κB激活高通量检测系统在急救中的应用
  • 批准号:
    23659294
  • 财政年份:
    2011
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of the hypersensitive and rapid detection method for NF-κB activation using by Fluorescence Correlation Spectroscopy and its application to the emergency medicine
荧光相关光谱超灵敏快速检测NF-κB活化方法的建立及其在急诊医学中的应用
  • 批准号:
    20590559
  • 财政年份:
    2008
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Rapid diagnosis of the systemic inflammatory response. syndrome by the transcription factor activation measuring method development concerning the inflammation
快速诊断全身炎症反应。
  • 批准号:
    17590486
  • 财政年份:
    2005
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Memory and learning disorder analysis using mutation mouse for mental retadation related gene ATRX and elucidation of the genetic control abnormality.
使用智力迟缓相关基因 ATRX 突变小鼠进行记忆和学习障碍分析,并阐明遗传控制异常。
  • 批准号:
    15500210
  • 财政年份:
    2003
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular mechanism of constitutive expression of interleukin 8 in the cancer progress and the development of the angiogenesis control therapy
白细胞介素8组成型表达在癌症进展中的分子机制及血管生成控制治疗的发展
  • 批准号:
    13670315
  • 财政年份:
    2001
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular constructions of oligodeoxynucleotides binding to bFGF targeting for angiogenesis
结合 bFGF 靶向血管生成的寡脱氧核苷酸的分子结构
  • 批准号:
    09470172
  • 财政年份:
    1997
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Gene therapy for atherosclerosis by inhibition of nuclear transcriptional factor
通过抑制核转录因子进行动脉粥样硬化的基因治疗
  • 批准号:
    07457174
  • 财政年份:
    1995
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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