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Gene expression of the pancreas in the patients with fulminant type 1 diabetes

暴发性1型糖尿病患者胰腺基因表达

基本信息

批准号：
17590950
负责人：
IMAGAWA Akihisa
金额：
$ 2.24万
依托单位：
Osaka Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590950/
关键词：
fulminant diabetes idiopathic cytokine interferon tumor necrosis factor interferon tumor necrosis factor

项目摘要

1) Extraction of total RNA from the preserved pancreatic tissue of type 1 diabetic patients and expression of inflammatory cytokinesWe extracted total RNA from the pancreatic tissue of type 1 diabetic patient that was taken by laparoscopic pancreas biopsy and have been preserved at-80C for several years. They were 6 patients (3 patients with fulminant diabetes and 3 patients with autoimmune type). We generated cDNA from the total RNA taken as above and examined the expression of pancreatic hormones and inflammatory cytokines by using semi-quantitive PCR methods. First of all, we confirmed the expression of insulin and glucagon gene by using these methods. Next, interferon-gamma was expressed in the pancreatic tissue of all 6 patients (3 patients with fulminant diabetes and 3 patients with autoimmune type). Tumor necrosis factor alpha was expressed in all 3 patients with fulminant diabetes but not in 3 patients with autoimmune type at all. These findings suggest the different profile of gene expression in the pancreatic tissue between fulminant and autoimmune types even if both of them are categorized within type 1 diabetes.2) Comprehensive analysis of the gene expression by using microarrayOn the basis of the above findings, we tried comprehensive analysis of the gene expression in the pancreatic tissue of type 1 diabetic patients by using microarray, but failed.

1)从保存的1型糖尿病患者胰腺组织中提取总RNA及炎性细胞因子的表达我们从腹腔镜胰腺活检取得的1型糖尿病患者胰腺组织中提取总RNA，并在-80℃下保存了数年。他们是6名患者（3名暴发性糖尿病患者和3名自身免疫型患者）。我们从上述提取的总RNA中生成了cDNA，并使用半定量PCR方法检查了胰腺激素和炎症细胞因子的表达。首先，我们利用这些方法确认了胰岛素和胰高血糖素基因的表达。接下来，干扰素-γ在所有 6 名患者（3 名暴发性糖尿病患者和 3 名自身免疫型患者）的胰腺组织中表达。肿瘤坏死因子 α 在所有 3 名暴发性糖尿病患者中均表达，但在 3 名自身免疫型糖尿病患者中完全不表达。这些发现提示暴发型和自身免疫型糖尿病患者的胰腺组织中的基因表达谱存在差异，即使两者都属于1型糖尿病。2）利用微阵列对基因表达进行综合分析基于上述发现，我们尝试利用微阵列对1型糖尿病患者的胰腺组织中的基因表达进行综合分析，但失败了。