Analysis of methylation status of the promoter of the IRF2 gene, a candidate gene for psoriasis

银屑病候选基因IRF2基因启动子甲基化状态分析

• 批准号: 17591180
• 负责人: FUKAI Kazuyoshi
• 金额: $ 2.24万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591180/
• 关键词: psoriasis; interferon; transcription factor; methylation; promoter; インターフェロン; エピジェネティックス

The interferon regulatory factor 2 gene is a strong candidate gene for type 2 psoriasis, because of the localization of the gene to chromosome 4q35, and the function of the protein to negatively regulate the secretion of interferon alpha and beta. The methylation of the promoter alters the expression of the gene and has been implicated as the cause for aging and cancer. The methylation status of the interferon regulatory factor 2 was investigated in DNA of peripheral leukocytes with type 2 psoriasis. Genomic DNAs of ten cases with old psoriasis were bisulfated. The promoter region of the IRF2 gene was PCR-amplified and cloned to TA cloning vector and sequenced. Approximately 20 clones were sequenced with each subject. However, it was demonstrated that the promoter of the IRF2 gene was not methylated in type 2 psoriasis. The proportion of the methylated CpG was about 0.5%. Thus, it was suggested that the methylation of the promoter of IRF2 gene was not associated with type 2 psoriasis.

干扰素调节因子2基因是2型银屑病的有力候选基因，因为该基因定位于染色体4q35，并且该蛋白具有负调节干扰素α和β分泌的功能。启动子的甲基化改变了基因的表达，并被认为是衰老和癌症的原因。研究了 2 型银屑病外周血白细胞 DNA 中干扰素调节因子 2 的甲基化状态。对 10 例陈旧性银屑病患者的基因组 DNA 进行了硫酸氢盐化。对IRF2基因的启动子区域进行PCR扩增并克隆至TA克隆载体并测序。对每个受试者大约 20 个克隆进行了测序。然而，已证明IRF2基因的启动子在2型银屑病中没有甲基化。甲基化CpG的比例约为0.5%。因此，提示IRF2基因启动子甲基化与2型银屑病无关。

项目成果

Deletion of distal promoter of VCXA in a patient with X-linked ichthyosis associated with borderline mental retardation

• DOI: 10.1016/j.jdermsci.2006.10.001
• 发表时间: 2007-01-01
• 期刊: JOURNAL OF DERMATOLOGICAL SCIENCE
• 影响因子: 4.6
• 作者: Hosomi, Naoko;Oiso, Naoki;Ishii, Masamitsu
• 通讯作者: Ishii, Masamitsu

TIE2 gain-of-function mutation in a patient with pancreatic lymphangioma associated with blue rubber bleb nevus syndrome

伴有蓝色橡胶泡痣综合征的胰腺淋巴管瘤患者的 TIE2 功能获得突变

• 发表时间: 2006
• 期刊: Surgery Today 36(3)
• 作者: Nobuhara Y;Onoda N;Fukai K 他
• 通讯作者: Fukai K 他

Mutation analysis of the ADAR1 gene in dyschromatosis symmmetrica hereditaria and genetic differentiation from both dyschromatosis universalis and acropigmentatio reticularis.

遗传性对称性色素异常症 ADAR1 基因的突变分析以及普遍性色素异常症和网状肢端色素沉着症的遗传分化。

• 发表时间: 2005
• 期刊: Journal of Investigative Dermatology 124(6)
• 作者: Suzuki N;Suzuki T;Inagaki K Ito S;Kono M;Fukai K et al.
• 通讯作者: Fukai K et al.

A case of acquired smooth muscle hamartoma of the scrotum

获得性阴囊平滑肌错构瘤1例

• 发表时间: 2005
• 期刊: Clinical and Experimental Dermatology 30(5)
• 作者: Shiraki Y;et al;Oiso N et al.
• 通讯作者: Oiso N et al.

The most updated therapy in Dermatology in2007-2008, Takigawa M and Watanebe S eds, Nankodo, Tokyo

2007-2008 年皮肤科最新疗法，Takikawa M 和 Watanebe S eds，Nankodo，东京

• 发表时间: 2007
• 作者: Xanthoma;Fukai K
• 通讯作者: Fukai K