Regeneration of periodontal ligaments in jawbone using materials driven by magnetic field

使用磁场驱动的材料再生颌骨中的牙周膜

• 批准号: 17592158
• 负责人: SHIMIZU Yoshinaka
• 金额: $ 1.47万
• 依托单位: Tohoku university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592158/
• 关键词: periodontal ligament; regeneration; materials driven by magnetic field

The aim of this study was to construct the connective structure of hard tissue by fibrous ligaments in dynamic environment. For the development of the medical appliance, we selected elemental components and aligned the amplitude and frequency of magnetic materials reacting to magnetic gradient. We used the mixture materials of epoxy resin and magnetic substance metals (cobalt ferrite powder). In experiment of subcutaneous implantation, the biocompatibility of this material was confirmed histopathologically and physically. The healing of bone groove in rat cranium was investigated in static and dynamic states. The grooves of about 0.5, 1.0, and 1.5 mm breadth through the dura mater were formed in rat cranium. Histological sections were histologically observed. The groove of 1.5 mm breadth was connected with fibrous connective tissue. The groove of 1.0 mm breadth was reconstructed by bone tissue. Subsequently, the isolated bone surrounded by groove of 1.0 mm breadth, which will come under the dynamic influence of blood pressure in cranial cavity, showed no bony connection. In a part, the Sharpey's fibers, which reveal collagen fibers or bundles embedded in calcified tissue, were seen on the isolated and the surrounding bone surfaces. It was suggested that the Sharpey's fibers were formed by dynamic effects. To confirm the formation of Sharpey's fiber structure in tooth socket of jawbone, sintered tooth was implanted in first molar tooth socket in rat maxilla. Implanted teeth were extracted from else rats, and sintered in 1100℃, and sterilized. First molar tooth was extracted. And soon sintered tooth was implanted in extracted tooth socket. Sintered tooth was connected to second molar with resin. Granulation and fibrous connective tissue with slight inflammatory cells infiltration was present between sintered bone and alveolar bone on 10 days after implantation. It was suggested that the long-term observation is necessary.

本研究的目的是在动态环境下利用纤维韧带构建硬组织的结缔组织。为了医疗器械的开发，我们选择了元素成分，并排列了磁性材料对磁梯度反应的幅度和频率。我们采用环氧树脂和磁性物质金属（钴铁氧体粉末）的混合材料。在皮下植入实验中，通过组织病理学和物理实验证实了该材料的生物相容性。在静态和动态两种状态下对大鼠颅骨骨沟愈合进行了研究。在大鼠头盖骨内形成约0.5、1.0和1.5 mm宽的硬脑膜沟。组织切片进行组织学观察。沟槽宽度为1.5 mm，与纤维结缔组织连接。用骨组织重建宽度为1.0 mm的骨沟。随后，被1.0 mm宽沟包围的离体骨受颅腔内血压的动态影响，无骨连接。在一部分中，在分离的骨表面和周围的骨表面可以看到Sharpey纤维，它可以显示钙化组织中嵌入的胶原纤维或束。Sharpey’s纤维是由动力作用形成的。为证实下颌骨牙窝Sharpey纤维结构的形成，在大鼠上颌第一磨牙窝植入烧结牙。取其他大鼠植牙，1100℃烧结，灭菌。拔除第一颗臼齿。很快将烧好的牙植入拔牙槽内。用树脂将烧结后的牙齿与第二磨牙连接。植入10 d后，烧结骨与牙槽骨间出现肉芽和纤维结缔组织，伴有轻微炎症细胞浸润。有人建议，长期观察是必要的。