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Low temperature adaptation of hyperthermophile

超嗜热生物的低温适应

基本信息

批准号：
17613007
负责人：
FUJIWARA Shinsuke
金额：
$ 2.37万
依托单位：
Kwansei Gakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Both Thermococcus and Pyrococcus species belong to the Thermococcales order and share various physiological features. They grow near or above at 100℃ and are strictly anaerobic heterotrophs that utilize a variety of carbon substrates, such as peptides and/or carbohydrates. The unique difference is the growth temperature range. For example, the minimum growth temperatures of Thermococcus spp. (ex. Thermococcus kodakaraensis, 60℃) are generally lower than those of Pyrococcus spp. (P.abyssi, 67℃ ; P.furiosus, 70℃ ; P.horikoshii, 80℃). Thermococcus kodakarensis possesses a single circular genome and 747 genes are unique in T.kodakaraensis that are not identified in Pyrococcus spp. Some of these genes are considered to be involved in low temperature survival in T. kodakaraensis. Transcriptome and proteome studies revealed that some genes are induced specifically at low temperature environment. Among them, we mainly focused on chaperonin CpkA and RNA helicase (Tk0306) and their induction pro … More files were analyzed. The expression of low temperature inducible chaperonin cpkA was regulated at the post-transcriptional level. In contrast, high temperature inducible chaperonin cpkB was regulated at the level of transcription. When we compare promoter sequences of cpkA and cpkB, only slight difference was observed, suggesting that these genes were evolved from the same common ancestor and only cpkA acquired low temperature inducible mechanism in the course of evolution. As for RNA helicase, it was regulated at the level of transcription. RNA helicase showed highest enzymatic activity at 50℃. It is unusual because Tkodakaraensis does not grow at the temperature. The RNA helicase seems functional in T. kodakaraensis only to survival at low temperature environments. Further study is needed to confirm the idea. Besides these two genes, enzymatic characteristics for isopentenyl diphasphate isomerase, glucosamine-6-phosphate deaminase, methylguanine-DNA methyltransferase and homing endnuclasell were examined. Less

Thermococcus和Pyrococcus属都属于Thermococcales目，具有多种生理特征。它们在接近或高于100℃的温度下生长，是严格的厌氧异养生物，利用各种碳底物，如肽和/或碳水化合物。唯一的区别是生长温度范围。例如，热球菌属（Thermococcus spp.）（前。Thermococcus kodakaraensis，60℃）的产率普遍低于Pyrococcus spp.（P.abyssi，67℃ ; P.furiosus，70℃ ; P.horikoshii，80℃）。Kodakarensis具有单一的环状基因组，747个基因在Kodakaraensis中是独特的，而在Pyrococcus spp.中没有被鉴定。其中一些基因被认为与T.科达卡拉转录组和蛋白质组的研究表明，一些基因在低温环境中受到特异性的诱导。其中，我们主要对分子伴侣CpkA和RNA解旋酶（Tk 0306）及其诱导蛋白进行了研究。 ...更多信息文件进行了分析。低温诱导型伴侣蛋白cpkA的表达在转录后水平受到调控。相反，高温诱导型伴侣蛋白cpkB在转录水平上受到调控。比较cpkA和cpkB的启动子序列，发现它们之间的差异很小，说明这两个基因是从同一个共同祖先进化而来的，只有cpkA在进化过程中获得了低温诱导机制。RNA解旋酶则在转录水平上受到调控。RNA解旋酶在50℃时活性最高。这是不寻常的，因为Tkodakaraensis不生长在温度。RNA解旋酶在T.只有在低温环境下才能存活。需要进一步的研究来证实这一想法。除这两个基因外，还检测了异戊烯基二磷酸异构酶、葡糖胺-6-磷酸脱氨酶、甲基鸟嘌呤-DNA甲基转移酶和归巢内切酶的酶学特性。少

项目成果

期刊论文数量（31）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes

DOI：
10.1101/gr.3003105
发表时间：
2005-03-01
期刊：
GENOME RESEARCH
影响因子：
7
作者：
Fukui, T;Atomi, H;Imanaka, T
通讯作者：
Imanaka, T

Crystal structure of intein homing endonuclease ll encoded in DNA polymerase gene from Hyperthermophilic Archaeon Thermococcus kodakaraensis strain KOD1.

来自超嗜热古细菌Thermococcus kodakaraensis 菌株KOD1 的DNA 聚合酶基因编码的内含肽归巢核酸内切酶II 的晶体结构。

DOI：
发表时间：
2006
期刊：
Proteins 63
影响因子：
0
作者：
Matsumura;H.
通讯作者：
H.

耐熱性酵素を利用したイソプレノイド化合物の合成

使用热稳定性酶合成类异戊二烯化合物

DOI：
发表时间：
2006
期刊：
バイオサイエンスとインダストリー 64・2
影响因子：
0
作者：
Takaya N;Katoh Y;Iwabuchi K;Hayashi I;Itoh S;Okumura K;Ra C;Nagaoka I;Daiba H.;H.Takeda;Fumihiko Tochinai;佐藤勝也;戸田山和久;斉藤了文;東端啓貴
通讯作者：
東端啓貴

Methods in Chemistry, Methods in Chemistry(the fifth edition)Vol.29 Principles of Biotechnology (DNA electrophoresis p139〜p149)

化学方法，化学方法（第五版）Vol.29 生物技术原理（DNA 电泳 p139-p149）

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
Suda T;Suzuki Y;Matsui T;Inoue T;Niide O;Yoshimaru T;Suzuki H;Ra C;Ochiai T.;礒崎初仁;Tanaka M.;小澤守;The Chemical Society of Japan
通讯作者：
The Chemical Society of Japan

Crystal structure of intein homing endonuclease II encoded in DNA polymerase gene from hyperthermophilic Archaeon Thermococcus kodakaraensis strain KOD1