Orthogonal functionalisation of pore interfaces for the generation of tailor-made biofunctionalised papers

该项目旨在制定以知识为基础开发用于生物医学和生物分析的纸质材料的战略。它定位于纸张分析和功能化领域，长期目标是在合理设计标准的基础上建立工具，用于定制具有预测的生物物理、流体和酶性质的生物功能化纸张。重点是开发将生物分子固定在纸上的特殊方法，以及有针对性地生产刺激响应微流控通道。使用常用的蛋白质与纸纤维的化学偶联方法，不可能将生物分子以限定的方向和高密度固定在纸表面上。这个问题通过两步程序的应用得到了解决，该程序依赖于纸纤维的预功能化，多肽作为蛋白质(酶、抗体)直接酶连接的锚定分子。本项目主要解决的问题是，哪种多肽修饰纤维素的偶联策略和哪种蛋白质的生物催化偶联策略可以获得更高的负载密度和生物活性。根据这些调查结果，本供资期间的一个特别重点将是编写更复杂的功能文件。首先，我们计划生成由不同功能化纤维类型组成的双官能化和寡官能化论文，这些纤维类型可以单独使用不同的生物分子。其次，所获得的知识将被应用于微流控生物活性试纸的生成，这些试纸可用于纸上实验室应用的框架中。

This project is aimed at establishing strategies for the knowledge-based development of paper materials for applications in biomedicine and bioanalytics. It is positioned in the field of paper analytics and functionalisation with the long-term goal to establish on the basis of rational design criteria tools for the tailor-made generation of biofunctionalized paper with predicted biophysical, fluidic, and enzymatic properties. The focus is on the development of special methods for the immobilisation of biomolecules on paper and the targeted production of stimulus responsive microfluidic channels. With commonly used methods for chemical conjugation of proteins to paper fibres, it is not possible to immobilise biomolecules in a defined orientation and in high density on the paper surface. This problem was solved by application of the two-step procedure that relies on pre-functionalisation of paper fibres with peptides that serve as anchor molecules for the directed enzymatic ligation of proteins (enzymes, antibodies). This project mainly aims at addressing the question, which conjugation strategy for cellulose modification with peptides and which biocatalytic conjugation strategy for proteins results in superior loading density and bioactivity. Based on these findings a special focus in this funding period will be on the generation of more complex functional papers. First, we plan to generated bi- and oligofunctionalised papers composed of different functionalised fibre types that can be individually addressed with different biomolecules. Second, the gained knowledge will be applied to the generation of microfluidic bioactive papers that can be used in the frame of lab-on-paper applications.