Development of semiconductor-based biosensing system for quality evaluation of embryo obtained by in vitro fertilization

开发基于半导体的生物传感系统，用于体外受精获得的胚胎质量评估

• 批准号: 22H03920
• 负责人: 坂田 利弥
• 金额: $ 11.23万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22H03920/
• 关键词: 半導体バイオセンサ; 体外受精卵; バイオセンシング; 半導体; バイオセンサ

半導体バイオセンサのゲート電極表面に細胞培養に適した化学修飾を施し細胞を培養する。その際、細胞とゲート電極との間に形成されるナノメートルオーダーのギャップに細胞呼吸により蓄積される乳酸や二酸化炭素といった代謝産物がpH変化を誘導する。つまり、この細胞/ゲート電極間のナノギャップ領域でのpH変化を半導体バイオセンサにより非侵襲的に計測可能となる。本来、半導体バイオセンサのゲート電極表面は酸化膜から構成されており、pH応答性が良好で電気化学的にはネルンスト応答（59.2 mV/pH at 25 ℃）を示す。しかしながら、現状ではゲート電極とその周辺部まで細胞が一面に培養されているため、個々の細胞に基づくpH変化を再現性良く定量的に見積もることが困難である。特に、個々の評価が必要な体外受精卵（胚）を対象とする場合、各ゲート電極上に体外受精後の胚を一つ一つ静置し長時間活性をモニタリングする必要がある。そこで本研究では、計測対象を胚とし、一つのゲート電極上に一つの胚が固定可能なウェルを含むカートリッジ型プレートを作製し、個々の胚の呼吸活性を再現良く定量的に評価する。当該年度は、マウス胚の呼吸活性を計測およびその形態を観察可能な透明ゲート半導体バイオセンサを作製するとともに、そのゲート電極にマウス胚1個を配置するためのウェルを作製した（カートリッジ型プレート）。また、上記カートリッジ型プレートを用いて電気計測の可能性について調査した。

Hemispherical materials are used to conduct cell culture on the surface of the cathode, chemical repair and cell culture. In the world, there is a lot of carbon dioxide in the air, and the pH is used as a substitute for lactic acid diacidizing. In the field of communication and communication, it is possible to reduce the cost of non-intrusive equipment in the field of pH. The acidified film on the surface of the electrode was formed into a thin film on the surface of the electrode, and the pH response was good. The temperature response of electrical chemistry was measured at 59.2 mV/pH at 25 ℃. On the one hand, the cell of the department of electrical power supply is responsible for the development of a basic pH for the purpose of improving the quality and quantity of environmental pollution. It is necessary to combine the embryos fertilized in vitro (embryos) in vitro, and to keep the embryos in place for a long time after in vitro fertilization. In this study, it is possible to evaluate the respiratory activity and respiratory activity of embryos in this study. in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: 1. During the year, when the respiratory activity of embryos is measured, it is possible to make sure that it is possible to make sure that it is transparent that the semiconductors are used to do so, and that there is a device that can be used for the purpose of monitoring the respiratory activity of embryos during the year. The possibility of the use of electrical equipment and electrical equipment in the upper and lower levels of the market.

项目成果

Effect of Surface Modification on the Fundamental Electrical Characteristics of Solution-Gated Indium Tin Oxide-Based Thin-Film Transistor Fabricated by One-Step Sputtering

• DOI: 10.1021/acs.langmuir.2c03225
• 发表时间: 2023-03-28
• 期刊: LANGMUIR
• 影响因子: 3.9
• 作者: Katayama，Ritsu;Sakata，Toshiya
• 通讯作者: Sakata，Toshiya