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Cellular and molecular research on male-sterile sugi, Cryptomeria japonica.

雄性不育柳杉的细胞和分子研究。

基本信息

批准号：
14560003
负责人：
FURUTA Yoshihiko
金额：
$ 2.37万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560003/
关键词：
Sugi, Cryptomeria japonica male - sterility pollen allergen pollen - allergy genetic resources genetic analysis diversity elite trees スギ実生無選抜クローン両性不稔異常花粉形成花粉アレルゲン富山県立山遺伝分析早期選抜生態的多様性

项目摘要

Sugi, Cryptomeria japonica is an endemic cedar species and most important forest resources, and have been daily necessities wood, wind - snow break trees of houses and landscape or symboric trees in Japan. After II war, sugi were planted whole mountain area except Hokkaidou, in order to supply of wood. Now, these trees are mature and exposure a lot of pollens in the planted areas. These pollen cause pollen allergy in many people in whole country in Spring with other factors. We discovered a single completely male-sterile sugi tree in Toyama, and indicated this male sterility causes by a single nucear recessive gene. In this program, we plan 1) utility of this male sterility, 2) survey of new male-sterile sugi plant and 3) survey of allergen-free pollen tree or allergen-less pollen tree from natural or elite tree populations.In the first and second years (2002 and 2003), we selected three male-sterile plants from 10,902 seedlings of Tateyama sugi. We confirmed these plants were propagat … More e easily by grafing. These selected trees will be available for advanced research.In order to develop 2) and 3) purposes mentioned above, we analyzed pollen fertility and measured content of pollen-allergen, Cry j 1 in 163 ellite clone trees collected from whole country except with Hokkaidou, 38 selected trees in Gifu prefecture and 84 trees derived from seeds near Gifu University in the third and the last years. Unfortunately, we did no discover completely male-sterile tree or allergen-free pollen tree, but two or three partial male-sterile trees probably owing to triploidy. These facts based on whole research results indicate the selection of male-sterile plants from seedlings is efficiency. It is no correlation between pollen fertility and allergen content per pollen. The data from same trees in two years show low year to year variation in pollen fertility, and no correlation in pollen allergen content between two years. The male-sterility is stable genetic characteristics, the latter is variable character. Less

杉木（Cryptomeria japonica）是日本特有的杉木树种，是日本最重要的森林资源，是日本的日用木材、房屋防风防雪树、园林绿化或象征树木。第二次世界大战结束后，为了供应木材，除了北海道以外的整个山区都种植了杉木。现在，这些树成熟了，在种植区域暴露了很多花粉。这些花粉与其他因素一起在春季引起全国许多人的花粉过敏。我们在富山发现了一株完全雄性不育的杉树，认为这种雄性不育是由单个核隐性基因引起的。在本项目中，我们计划1)利用这一雄性不育，2)调查新的雄性不育杉木植物，3)调查自然种群或精英种群中无过敏原或无过敏原的花粉树。在2002年和2003年，我们从立山杉的10,902株幼苗中选择了3株雄性不育植株。我们证实这些植物是繁殖的…更容易通过嫁接。这些选定的树木将用于高级研究。为了研究上述2)和3)目的，我们分析了除北海道以外的全国163棵卫星无性系树、岐阜县38棵树和岐阜大学附近84棵树的花粉育性和花粉过敏原Cry j 1的含量。不幸的是，我们没有发现完全雄性不育树或无过敏原的花粉树，但可能由于三倍体的原因，有2 - 3株部分雄性不育树。综合研究结果表明，从幼苗中选择雄性不育植株是有效的。花粉育性与每粒花粉中过敏原含量无相关性。同一树种2年的数据显示，花粉肥力的年际变化不大，花粉过敏原含量在2年之间无相关性。雄性不育是稳定的遗传特征，后者是可变的遗传特征。少