High-resolution tandem mass spectrometer

高分辨率串联质谱仪

• 批准号: 517100561
• 金额: --
• 依托单位: Universität Konstanz
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/517100561?language=en
• 关键词: resolution; tandem; mass; spectrometer

A high-resolution tandem mass spectrometer with ultra high performance liquid chromatography coupling for the untargeted analysis of complex biological and synthetic samples is applied for. Therefore, a mass spectrometer with high resolution, mass accuracy, mass stability, scan rate and a broad dynamic range is requested. To enable identification of unknown and known compounds, the instrument should enable acquisition of the high resolution mass, as well as MS/MS-spectra. Thus, a tandem mass spectrometer with electronspray (ESI)-ionsource and a quadrupol- time-of-flight detector (Q-ToF) is applied for. The liquid chromatography system should include a binary high pressure pump, an autosampler with cooling system, and a diode array detector. Besides untargeted analytical questions, the instrument will be utilized for the biochemical characterization of novel enzymes, and the activity-screening of enzyme variants for protein engineering projects.

高分辨率串联质谱仪与超高效液相色谱耦合，用于复杂生物和合成样品的非靶向分析。因此，要求质谱仪具有高分辨率、质量准确度、质量稳定性、扫描速率和宽动态范围。为了能够识别未知和已知化合物，仪器应能够采集高分辨率质量以及MS/MS光谱。因此，串联质谱仪与电喷雾（ESI）-离子源和四极杆-飞行时间检测器（Q-ToF）的应用。液相色谱系统应包括一个二元高压泵、一个带冷却系统的自动进样器和一个二极管阵列检测器。除了非目标分析问题外，该仪器还将用于新型酶的生物化学表征，以及蛋白质工程项目中酶变体的活性筛选。