Ultra High-Performance Liquid Chromatography High-Resolution Mass Spectrometer (UHPLC-HRMS)

超高性能液相色谱高分辨率质谱仪 (UHPLC-HRMS)

• 批准号: 529714284
• 金额: --
• 依托单位: Friedrich-Schiller-Universität Jena
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/529714284?language=en
• 关键词: Ultra; Performance; Liquid; Chromatography; Resolution

We apply for an Ultra High-Performance Liquid Chromatography High-Resolution Mass Spectrometer for the metabolomic investigation of microorganisms and microbial communities. The instrument will mainly serve to monitor the metabolic responses of microorganisms to different interaction partners within communities and to identify signaling metabolites that regulate the community structure. Model communities from laboratory experiments as well as samples from mesocosm and fieldwork will be investigated by exploiting the power of modern mass spectrometry. The instrument will contribute to a platform for several groups organized within the CRCs ChemBioSys and AquaDiva and it will be part of the instrument park of the EXC Balance of the Microverse. Metabolomics is a mature technique that allows the monitoring of metabolic changes within given biological systems. It serves as a method for hypothesis generation by identification of metabolic pathways that are regulated in response to external factors, such as stress or the presence of interaction partners. With powerful chemoinformatic support, large datasets can be mined for metabolites that are candidate chemical mediators. Especially the high resolution of modern mass spectrometers and the fast scan rate allows for the comprehensive characterization of metabolites. The sensitivity of the newest instrument generation will allow, on the one hand, to address even trace compounds within complex natural samples but also enable miniaturization and monitoring of samples generated, e.g., from microfluidics experiments. The resulting mass spectrometric data will also form the basis for the development of new chemoinformatics approaches for computer-aided structure elucidation. On the one hand, this instrument will thus advance research in method-developing analytical chemistry and support activities in the department of bioorganic analytics. On the other hand, it will also enable collaborative projects within larger consortia within the faculties of biosciences, faculty of chemistry and earth sciences and the faculty for mathematics and informatics. The device will thus have an important function at the Jena site as a platform technology for numerous research activities.

我们申请了一台超高效液相色谱高分辨率质谱仪，用于微生物和微生物群落的代谢组学研究。该仪器将主要用于监测微生物对社区内不同互动伙伴的代谢反应，并确定调节社区结构的信号代谢物。我们将利用现代质谱学的力量，对实验室实验中的模型群落以及中胚层和野外工作中的样本进行调查。该仪器将为在CRCS、ChemBioSys和AquaDiva内组织的几个小组提供一个平台，它将成为MicroVerse EXC天平仪器园的一部分。代谢组学是一项成熟的技术，可以监测特定生物系统内的代谢变化。它作为一种产生假说的方法，通过识别对外部因素(如压力或相互作用伙伴的存在)进行调节的代谢途径来生成假说。有了强大的化学信息学支持，可以从大数据集中挖掘出作为候选化学介体的代谢物。特别是现代质谱仪的高分辨率和快速扫描速度使代谢物的全面表征成为可能。最新一代仪器的灵敏度将允许，一方面，即使是复杂的自然样品中的痕量化合物，也可以实现小型化和监测产生的样品，例如，从微流体实验。由此产生的质谱学数据也将成为开发用于计算机辅助结构阐明的新的化学信息学方法的基础。一方面，这一仪器将因此促进方法研究--开发分析化学，并支持生物有机分析系的活动。另一方面，它还将在生物科学学院、化学和地球科学学院以及数学和信息学学院内的较大联合体内开展合作项目。因此，该装置将在耶拿现场发挥重要作用，作为众多研究活动的平台技术。