New Scope of RNA Functional Structures

RNA功能结构的新领域

• 批准号: 04272103
• 负责人: YOKOYAMA Shigeyuki
• 金额: $ 245.82万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04272103/
• 关键词: RNA; functional structure; splicing; ribozyme; ribosome; tRNA; NMR; X-ray crystallography; 高次構造; mRNAスプライシング; rRNA; RNA触媒(リボザイム)

The major results of the project for four years were presented in the 6th symposium on 24th of September, 1996. In the 6th symposium, as the results of the first "mechanism of RNA function expression", tertiary structures of RNA binding proteins involving the regulation of alternative splicing, class I aminoacyl-tRNA synthetases which perform precise tRNA recognition, and the animal mitochondrial tRNAs having characteristic secondary structures were presented. Furthermore, mechanism of activation of group I intron RNA,function of ribosomal RNA,and the molecular mimicry of tRNAs by proteins were also presented. As for the second project "investigation of new function of RNA", mechanism of trans-translation by the novel low molecular RNAs, communication mechanism between two domains in the ribosomal RNA,and function of mitochondrial RNAs on germ line maturation in Drosophila were presented. As the results of the third project "orign and evolution of RNA", the origin and evolution mechanism of aminoacyl-tRNA synthetases, ribozymes from virus, mitochondrial disease due to the mutation of mitochondrial tRNA genes were presented. These results were summarized in a printed report.

该项目历时四年的主要成果在1996年9月24日举行的第六届研讨会上发表。在第六届研讨会上，作为第一个“RNA功能表达机制”的成果，研究了涉及选择性剪接调节的RNA结合蛋白的三级结构、进行tRNA精确识别的I类氨酰tRNA合成酶、以及具有特征二级特征的动物线粒体tRNA。 提出了结构。此外，还介绍了I组内含子RNA的激活机制、核糖体RNA的功能以及蛋白质对tRNA的分子模拟。第二个项目“RNA新功能研究”，介绍了新型低分子RNA的反式翻译机制、核糖体RNA两个结构域之间的通讯机制以及线粒体RNA在果蝇种系成熟中的功能。作为第三个项目“RNA的起源与进化”的成果，提出了氨酰tRNA合成酶、病毒核酶、线粒体tRNA基因突变引起的线粒体疾病的起源和进化机制。这些结果总结在一份印刷报告中。

项目成果

T.Ohtsuki, G.Kawai, Y.Watanabe, K.Kita, K.Nishikawa and K.Watanabe: "Preparation of Biologically Active Ascaris suum Mitochondrial tRNAMet with a TV-Replacement Loop by Ligation of Chemically Synthesized RNA Fragments." Nucleic Acids Res.24. 662-667 (1996

T.Ohtsuki、G.Kawai、Y.Watanabe、K.Kita、K.Nishikawa 和 K.Watanabe：“通过化学合成的 RNA 片段连接，制备具有 TV 替换环的生物活性猪蛔虫线粒体 tRNAMet。”

S.Watanabe, G.Kawai, Y.Muto, K.Watanbe, T.Inoue and S.Yokoyama: "An RNA Fragment Consisting of the P7 and P9.0 Stems and the 3'-Terminal Guanosine of the Tetrahymena Group I Intron." Nucleic Acids Res.24. 1337-1344 (1996)

S.Watanabe、G.Kawai、Y.Muto、K.Watanbe、T.Inoue 和 S.Yokoyama：“由 P7 和 P9.0 茎以及四膜虫 I 组内含子的 3 末端鸟苷组成的 RNA 片段

Watanabe,A.: "The Role of Exon Sequence in Splice Site Selection" Gene & Development. 7. 407-418 (1993)

Watanabe,A.：“外显子序列在剪接位点选择中的作用”基因

Suzuki,T.: "The gene for serine tRNA having anticodon sequence CAG in pathogenic yeast,Candida albicans" Nucleic Acids Res.21. 356 (1993)

Suzuki,T.：“致病性酵母白色念珠菌中具有反密码子序列 CAG 的丝氨酸 tRNA 基因”Nucleic Acids Res.21。

Yoshioka,Y.: "Determination of the Phylogenetic Relationships among Pacific salmonide using Short Interspersed Elements (SINEs) as Temporal Landmarks of Evolution" Proc.Natl.Acad.Sci.U.S.A.90. 6995-6999 (1993)

Yoshioka,Y.：“使用短散布元素（SINE）作为进化的时间地标确定太平洋鲑鱼之间的系统发育关系”Proc.Natl.Acad.Sci.U.S.A.90。