New Scope of RNA Functional Structures

RNA功能结构的新领域

• 批准号: 04272103
• 负责人: YOKOYAMA Shigeyuki
• 金额: $ 245.82万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04272103/
• 关键词: RNA; functional structure; splicing; ribozyme; ribosome; tRNA; NMR; X-ray crystallography; 高次構造; mRNAスプライシング; rRNA; RNA触媒(リボザイム)

The major results of the project for four years were presented in the 6th symposium on 24th of September, 1996. In the 6th symposium, as the results of the first "mechanism of RNA function expression", tertiary structures of RNA binding proteins involving the regulation of alternative splicing, class I aminoacyl-tRNA synthetases which perform precise tRNA recognition, and the animal mitochondrial tRNAs having characteristic secondary structures were presented. Furthermore, mechanism of activation of group I intron RNA,function of ribosomal RNA,and the molecular mimicry of tRNAs by proteins were also presented. As for the second project "investigation of new function of RNA", mechanism of trans-translation by the novel low molecular RNAs, communication mechanism between two domains in the ribosomal RNA,and function of mitochondrial RNAs on germ line maturation in Drosophila were presented. As the results of the third project "orign and evolution of RNA", the origin and evolution mechanism of aminoacyl-tRNA synthetases, ribozymes from virus, mitochondrial disease due to the mutation of mitochondrial tRNA genes were presented. These results were summarized in a printed report.

该项目已有四年的主要结果在1996年9月24日举行的第六次研讨会上。提出了结构。此外，还介绍了蛋白质的I组内含子RNA的激活机理，核糖体RNA的功能和TRNA的分子模仿机理。至于第二个项目“ RNA的新功能的研究”，提出了新型低分子RNA的跨翻译机制，核糖体RNA中两个结构域之间的通信机制以及果蝇中线粒体RNA在生殖线成熟中的功能。作为第三个项目“ RNA的Orign和Evolution”的结果，氨基酰基-TRNA合成酶的起源和进化机制，病毒的核酶，由于线粒体TRNA基因突变而引起的线粒体疾病。这些结果总结在印刷报告中。

项目成果

Watanabe,K.: "Unusual anticodon loop structure found in E. coli Lysine tRNA" Nucl. Acids Res.22. 115-123 (1994)

Watanabe,K.：“在大肠杆菌赖氨酸 tRNA 中发现不寻常的反密码子环结构”Nucl。

Nameki,N.: "Idetity elements of Saccharomyces cerevisiae tRNA^<His>" Nucleic A cids Res. 23. 389-394 (1995)

Nameki,N.：“酿酒酵母 tRNA^<His> 的身份元件”核酸研究。

Hirao,I.: "Most compact hairpin-turn structure exerted by a short DNA fragment, d(GCGAAGC) in solution: an extraordinarily stable structure resistant to nucleases and heat" Nucl. Acids Res.22. 576-582 (1994)

Hirao,I.：“由短 DNA 片段 d(GCGAAGC) 在溶液中产生的最紧凑的发夹转角结构：一种对核酸酶和热具有抵抗力的极其稳定的结构”Nucl。

Sakamoto,K.: "A modified uridine in the first position of the anticodon of a minor species of arginie tRNA,the argU gene product,from Escherichia coli" Eur.J.Biochem.216. 369-375 (1993)

Sakamoto,K.：“来自大肠杆菌的 argU 基因产物，一种精氨酸 tRNA 的反密码子第一个位置上的修饰尿苷”Eur.J.Biochem.216。

Inagaki,Y.: "Lack of Peptide-Release activity Responding to Codon UGA in Mycoplasma Capricolum" Nucleic Acids Res.21. 1335-1338 (1993)

Inagaki，Y.：“缺乏响应山羊支原体中密码子 UGA 的肽释放活性”核酸研究 21。