培養線維芽細胞の力学的環境への機能的適応過程

培养成纤维细胞对机械环境的功能适应过程

• 批准号: 05221216
• 负责人: 高久田 和夫
• 金额: $ 1.79万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05221216/
• 关键词: 生体力学; 線維組織; 培養線維芽細胞; 力学的環境; 機能的適応

線維芽細胞をコラーゲンゲル内で培養し,機械的刺激のもとでの細胞の挙動を顕微鏡用VTR装置にて観察した.細胞が力学的環境を認識する機序,および細胞の張力発現などの機械的応答の過程について検討した結果,以下のことが明らかとなった.1.細胞が増殖し張力を発現しているコラーゲンゲルにメスで切れ目を入れ,張力の解放にともなう細胞の挙動を観察した.切れ目に垂直に並んでいた細胞は,時間の経過とともに切れ目に平行に配列した.この配向はゲル内の引張応力方向に一致するものであるが,個々の細胞が応力方向を認識するというよりも,細胞の発現する引張応力による全体的なコラーゲンゲルの収縮の寄与が最も大きく,さらに細胞の遊走や増殖の効果も働くものと判断された.実際に細胞数が少ない場合には配向は見られず,多数の細胞が協調して生じる力学的なオートクリン効果とも言える現象であることが明らかにされた.2.コラーゲンゲルのクランプ治具を製作し,細胞に繰返し伸縮の刺激を与えた際の力学的効果を調べた.培養期間の経過とともに細胞は応力方向に配向しマトリックスの剛性は上昇するが,静置群と比較して伸縮群では細胞の配向も剛性の上昇も顕著であり,細胞が培養下でも力学的環境に応じて適応することが確かめられた.3.上の実験で得られた試験片を引張試験し,培養組織の力学的性質が力学的環境に応じてどのように変化するかを定量的に調べた.弾性係数と最大引張応力の両者とも,伸縮群が静置群よりも顕著に大きかった.しかし培養期間が長くなると組織内での弾性係数の不均一性が著しくなり,引張試験を行うことができなくなった.顕微鏡下で微細組織の引張試験を行えばこのような困難は避けられるので,今後はこの点について改良を加える予定である.

The cell culture of line cells must be carried out in vitro, and the mechanical stimulation of the cells must be monitored by VTR microscopes. The mechanism of understanding the mechanical environment of cells, including the mechanical response process of cell tension development, is discussed in the following ways. 1. Cell proliferation, tension development, and cell movement are observed. The vertical direction of the eye is parallel to the horizontal direction of the eye, and the horizontal direction of the eye is parallel to the horizontal direction of the eye. The orientation of the orientation is the same as that of the tension force in the cell. The orientation of the tension force in the cell is the same as that in the cell. The orientation of the tension force in the cell is the same as that in the cell. The orientation of the tension force in the cell is the same as that in the cell. The orientation of the tension force in the cell is the same as that in the cell. The orientation of the contraction force in the cell is the same as that in the cell. The orientation of the tension force in the cell is the same as that in the cell. The orientation of the tension force in the cell is the same as that in the cell. The orientation of the tension force in the cell is the same as that in the cell. The orientation of the contraction force in the cell is the same as that in the cell. When the number of cells is small, the orientation of the cells is opposite, and most of the cells are coordinated. The mechanical effects of the cells are different. During the culture period, the orientation of the cells in the direction of the force increases, and the orientation of the cells in the static group increases. The mechanical properties of cultured tissues are regulated quantitatively by the mechanical environment. The coefficient of elasticity and the maximum tension force are the same, and the expansion group and the static group are different. The heterogeneity of the coefficient of permeability in the tissue during the culture period is very high. Microscopic micro-tissue tension test to avoid difficulties in the future to improve the point of increase in the predetermined.

项目成果

高久田和夫,宮入裕夫: "機械的刺激による線維芽細胞の自己組織化" 応用力学連合講演会講演論文集. 43巻. 629-630 (1994)

Kazuo Takakuda、Hiroo Miyairi：“机械刺激下的成纤维细胞自组织”应用力学联盟会议记录，第 43 卷，629-630（1994 年）。

高久田和夫,宮入裕夫: "培養線維芽細胞の力学的環境への機能的適応過程" 日本機械学会講演論文集. (1994)

Kazuo Takakuda、Hiroo Miyairi：“培养的成纤维细胞对机械环境的功能适应过程”，日本机械工程师学会论文集（1994 年）。