The role of protein disulfide isomerase for sorting glutelin and prolamin polypeptides in rice.

蛋白质二硫键异构酶在分选水稻中谷蛋白和谷醇溶蛋白多肽中的作用。

• 批准号: 14560077
• 负责人: OGAWA Masahiro
• 金额: $ 1.92万
• 依托单位: Yamaguchi Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560077/
• 关键词: rice; mutant; protein disulfide isomerase; PDI; molecular chaperon; Kinmaze; esp2; endosperm; 分子チャペロン; esp2変異; ジスルフィド結合; ジルフィド結合; プロテインジスフィドイソメラーゼ

Analysis of the full length of cDNA clone encoding 60kDa protein disulfide isomerase (PDI) was done. The 60kDa PDI consists of 511 amino acid residues and it has KDEL sequence as a luminal resitent signal. The genomic analysis of 60 kDa PDI shows that it comprises 10 exons with 9 introns intervals. The novel PDI with 40kDa was also cloned and it consists of 441 amino acid residues and has NDEL sequence as a luminal resident signal. The homology between the two PDIs shows about 40%, but both of them have two thioredoxin binding sites which are present in PDI enzyme. The rice mutant esp2 has no 60kDa PDI and the genetical analysis of Esp genes from allelic lines demonstrates that the Esp gene encodes 60kDa PDI. The enzymatic activities of 60kDa and 40kDa PDIs in a wild and esp2 mutant rices weredetermined ans showed that 60% and 40% of total PDI activities in wild rice comes from 60kDa and 40kD PDIs, respectively, while the 60kDa PDI activity is not seen in the esp2 mutant, meaning that 100% of PDI activity derive from 40kDa PDI alone. The immunolocalization of both PDIs shows that the 60kDa PDI is mainly present in the endpsperm, while the 40kDa PDI both in endosperm and aleurone layer.

对编码60kDa蛋白二硫异构酶（PDI）的cDNA克隆进行了全长分析。60kDa的PDI由511个氨基酸残基组成，具有KDEL序列作为光阻信号。对60 kDa PDI的基因组分析表明，它由10个外显子和9个内含子间隔组成。同时克隆出40kDa的新型PDI，它由441个氨基酸残基组成，具有NDEL序列作为腔内常驻信号。两个PDI的同源性约为40%，但它们都有两个硫氧还蛋白结合位点，这些位点存在于PDI酶中。水稻突变体esp2不具有60kDa PDI，对等位系Esp基因的遗传分析表明Esp基因编码60kDa PDI。测定了野生稻和esp2突变体中60kDa和40kDa PDI的酶活性，结果表明，野生稻中60%和40%的PDI活性分别来自60kDa和40kD PDI，而esp2突变体中没有60kDa PDI活性，这意味着100%的PDI活性来自40kDa PDI。两种PDI的免疫定位表明，60kDa的PDI主要存在于终精子中，而40kDa的PDI同时存在于胚乳和糊粉层中。

项目成果

The rice mutant esp2 greatly accumulates the glutelin precursor and deletes the protein disulfide isomerase

• DOI: 10.1104/pp.010624
• 发表时间: 2002-04-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Takemoto, Y;Coughlan, SJ;Kumamaru, T
• 通讯作者: Kumamaru, T

Takemoto, Y. et ad.: "The Rice Mutant esp2 Greatly Accumulates the Glutelin Precursor And Deletes the Protein Disulflde Isomerase"Plant Physiology. 128. 1212-1222 (2002)

Takemoto, Y. 等人：“水稻突变体 esp2 极大地积累了谷蛋白前体并删除了蛋白质二硫键异构酶”植物生理学。

Targeting of proteins to endoplasmic reticulum-derived compartments in plants. The importance of RNA localization

• DOI: 10.1104/pp.104.048934
• 发表时间: 2004-11-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Crofts, AJ;Washida, H;Satoh, H
• 通讯作者: Satoh, H

Targeting of Proteins to Endoplasmic Reticulum-Derived Compartments in Plants. The Importance of RNA Locarization.

蛋白质靶向植物内质网衍生的区室。

• 发表时间: 2004
• 期刊: Plant Physiology Vol.136
• 作者: Crowft;A.J.;et al.
• 通讯作者: et al.

Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene

水稻谷蛋白等位基因 GluA-1 基因的生化和分子特征

• 发表时间: 2003
• 期刊: Theoretical and Applied Genetics 107
• 作者: Qu;L.Q.et al.
• 通讯作者: L.Q.et al.