Biosynthetic Pathways of Highly Active Gibberellins

高活性赤霉素的生物合成途径

• 批准号: 14560087
• 负责人: NAKAYAMA Masayoshi
• 金额: $ 1.98万
• 依托单位: National Agriculture and Bio-Oriented Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560087/
• 关键词: Highly active gibberellin; Gibberellin biosynthesis; GA_3; GA_7; 11β-Hydroxyation; ジベレリン; GA_<120>; 生合成; 代謝; オーニソガラム; モモ

Deuterium-labeled GA_<95> was metabolized to deuterium-labeled GA_3 in immature seeds of Prunus persica. Both GA_3 and GA_<95> were detected in the tissue as endogenous gibberellins so that GA_<95> is a biosynthetic precursor of GA_3. Deuterium-labeled GA_<120> was metabolized to deuterium-labeled GA_7 in flower buds of Ornithogalum thylsoides. Both GA_7 and GA_<120> were detected in the inflorescence as endogenous gibberellins so that GA_<120> is a biosynthetic precursor of GA_7. Novel biosynthetic pathways that lead to highly active gibberellins of GA_3 and GA_7 by 3β-hydroxylation following 1, 2-double bond introduction are operating in P.persica and O.thylsoides. A biosynthetic step on the 1, 2-double bonds introduction into gibberellins could not be indicated in this research.Endogenous gibberellins in immature seeds of Eriobotrya japonica were analyzed and GA_9,GA_<15>,GA_<20>,GA_<24>,GA_<35>,GA_<44>,GA_<50>,GA_<61>,GA_<80> and GA_<84> were identified. Furthermore four new gibberellins, 11β-hydroxy GA_<12>,11β-hydroxy GA_<15>,11β-hydroxy GA_<53> and 11β-hydroxy GA_<20>, were identified and these were respectively assigned as the trivial numbers GA_<133>,GA_<134>,GA_<135> and GA_<136>. Enzymes catalyzing 11β-hydroxylation of gibberellins are actively operating in the tissue.

在桃李未成熟种子中，氘标记GA_<95>被代谢为氘标记GA_3。GA_3和GA_<95>均作为内源性赤霉素存在于组织中，说明GA_<95>是GA_3的生物合成前体。氘标记GA_<120>在鸟心草（Ornithogalum thylsoides）花蕾中代谢为氘标记GA_7。在花序中检测到GA_7和GA_<120>均为内源赤霉素，说明GA_<120>是GA_7的生物合成前体。新的生物合成途径通过引入1,2 -双键后，通过3β-羟基化产生高活性的赤霉素GA_3和GA_7。在本研究中，尚不能指出将1,2 -双键引入赤霉素的生物合成步骤。对粳米赤霉素进行了内源分析，鉴定出GA_9、GA_<15>、GA_<20>、GA_<24>、GA_<35>、GA_<44>、GA_<50>、GA_<61>、GA_<80>和GA_<84>。此外，还鉴定出了4个新的赤霉素，分别为11β-羟基GA_<12>、11β-羟基GA_<15>、11β-羟基GA_<53>和11β-羟基GA_<20>，并分别赋值为平凡数GA_<133>、GA_<134>、GA_<135>和GA_<136>。催化赤霉素11β-羟基化的酶在组织中活跃运作。

项目成果

A Major Result in 2004 in National Agriculture and Bio-Oriented Research Organization, National Institute of Floricultural Science

2004年国家农业与生物导向研究机构、国立花卉科学研究所重大成果

• 期刊: Plant Hormone Information : htpp://www.plant-hormones.info/gainfo.asp

A major Result in 2004 in National Agriculture and Bio-Oriented Research Organization, National Institute of Fruit Tree Science

2004年国家农业与生物导向研究机构、国立果树科学研究所重大成果

• 期刊: Plant Hormone Information : htpp://www.plant-hormones.info/gainfo.asp