权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Research for the regulation of large bowel fermentation of resistant starch and its application for the experimental colitis

抗性淀粉大肠发酵调控及其在实验性结肠炎中的应用研究

基本信息

批准号：
14560097
负责人：
MORITA Tatsuya
金额：
$ 2.18万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Our aim was to examine the prophylactic effect of a dietary high-amylose cornstarch (HAS) against trinitrobenzene sulfonic acid (TNBS)-induced rat colitis, focusing specifically on the changes in mucosal barrier function through the large bowel SCFA production. Male Wistar rats were fed a HAS-free basal diet or, 15% or 30% HAS supplemented diet. After 10 days, they were received intracolonic TNBS to induce colitis and further fed the respective diets for 8 days. At autopsy, colonic myeloperoxidase activities were determined as a marker of colonic injury. Fecal Ig A excretions and cecal short-chain fatty acids (SCFA) were also determined. In further experiment, fecal and cecal mucin contents, and colonic permeability were measured in rats fed the respective diets for 10 days. HAS ingestion significantly protected the increase in the colonic mycloperoxidase activities in rats at 8 days after TNBS administration, and there was a significant relationship between the mycloperoxidase activity and the degree of colonic injury. Rats fed the HAS diet showed a greater cecal SCFA production as measured by pool size than in those fed the basal diet. Fecal IgA excretions were also significantly greater in rats fed the HAS diet. Further, HAS ingestion dose-dependently increased the fecal and cecal mucin contents, protein, DNA and RNA contents in the colonic mucosa, and reduced the colonic permeability as measured by Evans-blue incorporation into the colonic tissue. These findings support the view that the prophylactic effect of HAS ingestion on TNBS-induced colitis may be exerted through the enhancement of mucosal barrier functions possibly due to the large bowel SCFA production.

我们的目的是研究膳食高直链淀粉玉米淀粉（HAS）对三硝基苯磺酸（TNBS）诱导的大鼠结肠炎的预防作用，特别关注通过大肠SCFA产生的粘膜屏障功能的变化。雄性Wistar大鼠喂食不含HAS的基础饮食或添加15%或30% HAS的饮食。10天后，它们接受结肠内TNBS以诱导结肠炎，并进一步喂食相应的饮食8天。尸检时，结肠髓过氧化物酶活性被确定为结肠损伤的标志物。还测定了粪便IG A排泄量和盲肠短链脂肪酸（SCFA）。在进一步的实验中，在喂食相应饮食10天的大鼠中测量粪便和盲肠粘蛋白含量以及结肠渗透性。在TNBS给药后第8天，HAS可显著保护大鼠结肠肌过氧化物酶活性的升高，且肌过氧化物酶活性与结肠损伤程度有显著相关性。喂食HAS饮食的大鼠表现出更大的盲肠SCFA生产，如通过池大小测量的，比那些喂食基础饮食的大鼠。粪便伊加排泄也显着更大的大鼠喂食HAS饮食。此外，HAS摄入剂量依赖性地增加结肠粘膜中的粪便和盲肠粘蛋白含量、蛋白质、DNA和RNA含量，并降低结肠渗透性，如通过埃文斯蓝掺入结肠组织所测量的。这些发现支持了这样的观点：摄入HAS对TNBS诱导的结肠炎的预防作用可能是通过增强粘膜屏障功能（可能是由于大肠SCFA的产生）来发挥的。