Research on the mechanism of stimulated mucin secrefion by dietary fiber ingestion and its physiological effects

本研究旨在阐明膳食纤维刺激粘蛋白分泌的机制及其生理效应。综上所述，本研究计划明确了以下几点：1。小肠和结肠粘蛋白的分泌与饮食中不消化成分在水中的沉降体积（体积形成特性的数值表示）成比例。不溶性膳食纤维刺激的粘蛋白分泌在开始摄入IDF后第5天达到最大值，停止摄入不溶性膳食纤维后第5天消失，表明这种刺激作用与上皮细胞的补充周期相一致.作为粘蛋白标记物的O-连接寡糖链的测量与通过新建立的ELISA直接测定的粘蛋白高度相关。不溶性膳食纤维可显著增加管腔粘蛋白含量，但管腔粘蛋白分泌能力的增加本身并不影响营养吸收。

The aim of this study was to clarify the mechanism of stimulated mucin secretion by dietary fiber ingestion and its physiological effects. In summary, the present research program clarified the followings:1. The small intestinal and colonic mucins are secreted in proportion to the settling volume in water (a numerical representation for bulk-forming property) of dietary indigestible components.2. Stimulated mucin secretion by insoluble dietary fiber reaches its maximum at 5 days after starting IDF ingestion and disappears at 5 days after ceasing insoluble dietary fiber ingestion, suggesting that this stimulatory effect is accordance with the replenishing cycle of epithelial cells.3. The measurement of O-linked oligosaccharide chain as a mucin marker is highly correlated with mucins directly determined by a newly established ELISA.4. Insoluble dietary fiber exerts a significant increase in luminal mucin content, but the increased capacity of luminal mucin secretion itself does not disturb the nutrient absorption.