CpG oligodeoxynucleotides enhance the non-specific Immune responses on carp

CpG寡脱氧核苷酸增强鲤鱼的非特异性免疫反应

• 批准号: 14560156
• 负责人: SAKAI Masahiro
• 金额: $ 2.3万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560156/
• 关键词: DNA; CpG; Carp; immune response; cytokine; CpG; 免疫賦活; リゾチーム; 白血球

The synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG were evaluated for its ability to enhance the non-specific immune response in carp, Cyprinus carpio. Intraperitoneal injection of CpG-ODNs to fish daily, 3. days, resulted in enhanced responses of phagocytic and nitroblue tetrazolium (NBT) activities in kidney phagocytic cells. This activation of kidney cells was observed for at least 7 days post injection. The serum lysozyme activity also increased in the fish treated with CpG-ODNs. These results indicated that unmethylated CpG DNA enhance the non-specific immune response in carp.

对合成的含有未甲基化CpG的寡核苷酸(ODN)增强鲤鱼非特异性免疫应答的能力进行了评价。连续3天给鱼腹腔注射CpG-ODN，可增强小鼠肾脏吞噬细胞的吞噬活性和NBT活性。这种肾脏细胞的激活在注射后至少7天内被观察到。CpG-ODNS处理组的血清溶菌酶活性也显著高于对照组。这些结果表明，未甲基化的CpG DNA增强了鲤鱼的非特异性免疫应答。

项目成果

A.C.M.A.R.Tassakka, N.Sakai: "CpG oligodeoxynucleotides enhance the non-specific Immune responses on carp, Cyprinus carpio"Aquaculture. 209, 1. 1-10 (2002)

A.C.M.A.R.Tassakka、N.Sakai：“CpG 寡脱氧核苷酸增强鲤鱼、Cyprinus carpio 的非特异性免疫反应”水产养殖。

A.C.M.A.R.Tassakka, M.Sakai: "CpG oligodeoxynucleotides enhance the non-specific immune responses on carp, Cyprinus carpio"Aquaculture. 209. 1-10 (2002)

A.C.M.A.R.Tassakka、M.Sakai：“CpG 寡脱氧核苷酸增强鲤鱼、Cyprinus carpio 的非特异性免疫反应”水产养殖。

Tassakka A.C.M.A.R., Sakai M.: "CpG oligodeoxynucleotides enhance the non-specific immune responses of carp, Cyprinus carpio"Aquaculture. 209(1). 1-10 (2002)

Tassakka A.C.M.A.R.，Sakai M.：“CpG 寡脱氧核苷酸增强鲤鱼、Cyprinus carpio 的非特异性免疫反应”水产养殖。

A.C.M.A.R.Tassakka, N.Sakai: "The in vitro effect of CpG-ODNs on the innate immune Responses of common carp, Cyprinus carpio L"Aquaculture. 220, 1. 27-36 (2003)

A.C.M.A.R.Tassakka、N.Sakai：“CpG-ODN 对鲤鱼先天免疫反应的体外影响”水产养殖。

A.C.M.A.R.Tassakka, M.Sakai: "The in vitro effect of CpG-ODNs on the innate immune responses of common carp, Cyprinus carpio L."Aquaculture. 220. 27-36 (2003)

A.C.M.A.R.Tassakka、M.Sakai：“CpG-ODN 对鲤鱼先天免疫反应的体外影响。”水产养殖。