Characterization of type 1 interferon in common carp Cyprinus carpio
鲤鱼 1 型干扰素的特性
基本信息
- 批准号:20580206
- 负责人:
- 金额:$ 3.08万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2008
- 资助国家:日本
- 起止时间:2008 至 2010
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The type I interferon (I-IFN) gene has recently been cloned and sequenced in the common carp species Cyprinus carpio L. Carp I-IFN cDNA is composed of 675 base pairs and is translated into a protein of 186 amino acid residues. The carp I-IFN encodes a predicted signal peptide of 23 amino acid residues and contains the I-IFN family signature His^<140>-Trp^<158>. Analysis of the homology between carp I-IFN and other known I-IFN and type II interferon (II-IFN) family members has revealed significant similarities to grass carp I-IFN. Phylogenetic analysis demonstrated that carp I-IFN clusters with I-IFN in teleosts, away from the other II-IFN family members. In addition, the gene structure for carp I-IFN is composed of 5 exons and 4 introns, a composition that is similar to that of the teleost I-IFN gene. RT-PCR analysis did not reveal gene expression in un-stimulated tissues including intestine, liver, gill, head kidney, muscle, spleen, mid-kidney and skin. However, I-IFN expression levels increased following stimulation with imiquimod in the head kidney cells. Furthermore, recombinant carp I-IFN protein (mature form) produced via the cell-free protein synthesis system stimulated the expression of the interferon-inducible Mx gene in the head kidney cells.
鲤鱼I型干扰素(I-IFN)基因已被克隆并测序。鲤鱼I-IFN cDNA由675个碱基对组成,翻译成186个氨基酸残基的蛋白质。鲤鱼I-IFN编码23个氨基酸残基的预测信号肽,并含有I-IFN家族特征His^<140>-Trp^<158>。鲤鱼I-IFN与其他已知的I-IFN和II型干扰素(II-IFN)家族成员之间的同源性分析显示,草鱼I-IFN具有显着的相似性。系统发育分析表明,鲤鱼I-IFN与硬骨鱼类I-IFN聚在一起,远离其他II-IFN家族成员。此外,鲤鱼I-IFN基因结构由5个外显子和4个内含子组成,其组成与硬骨鱼I-IFN基因的组成相似。RT-PCR分析未显示基因表达在未刺激的组织,包括肠,肝,鳃,头肾,肌肉,脾,中肾和皮肤。然而,I-IFN的表达水平增加后,咪喹莫特在头肾细胞的刺激。此外,重组鲤鱼I-IFN蛋白(成熟形式)通过无细胞蛋白质合成系统产生的刺激干扰素诱导的Mx基因在头肾细胞中的表达。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Characterization and expression analysis of type 1 interferon in common carp Cyprinus carpio
鲤鱼1型干扰素的特性及表达分析
- DOI:
- 发表时间:2009
- 期刊:
- 影响因子:0
- 作者:Kitao;Y.;Kono;T.;Korenaga;H.;Iizasa;T.;Nakamura;K.;Savan;R.;Sakai;M.
- 通讯作者:M.
Two isoforms of type1 interferon geneisolated from the common carp Cyprinus carpio L.
从鲤鱼 Cyprinus carpio L 中分离得到的两种 1 型干扰素亚型。
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:Kitao;Y.;Kono;T.;Sakai;M.
- 通讯作者:M.
Two isoforms of type I interferon gene isolated from the common carp Cyprinus carpio L.
从鲤鱼 Cyprinus carpio L 中分离得到的 I 型干扰素基因的两种亚型。
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:Kitao;Y.;Kono;T.;Sakai;M.
- 通讯作者:M.
Two isoforms of type1 interferon gene in the common carp Cyprinus carpio L.
鲤鱼Cyprinus carpio L中1型干扰素基因的两种亚型。
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:Sakai;M.;Kitao;Y.;Kono;T.
- 通讯作者:T.
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SAKAI Masahiro其他文献
SAKAI Masahiro的其他文献
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