The analysis of expressed genes in fish injected with immunostilumant

注射免疫增强剂鱼的表达基因分析

• 批准号: 11660193
• 负责人: SAKAI Masahiro
• 金额: $ 2.24万
• 依托单位: Miyazaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660193/
• 关键词: flounder; immunostimulant; gene; CC chemokine; EST; 免疫賦活剤; グルカン

Expressed sequence tags (ESTs) were obtained from the kidney of Japanese flounder Paralichthys olivaceus injected with peptidoglycan. The results of single-pass sequencing of ESTs from 198 clones from kidney cDNA will be presented. Sequences of the cDNA clones were compared with sequences in the GenBank database. Eighty and eight clones (44.4 %) appeared to be completely unknown and are likely to represent newly described genes, whereas 110 clones (55.6%) were identified based on matches to sequences in the database. The results contain the genes such as alpha globin, several ribosomal proteins, alpha collagen, heat shock protein 90 and cytochrome oxidase subunit. Immune related cDNAs identified from the kidney were immunoglobulin heavy and light chain, β2-microglobulin, thymosin β and lysozyme, MHC class 11α.

从注射肽聚糖的牙鲆肾脏中获得表达序列标签（ESTs）。将提供来自肾cDNA的198个克隆的EST的单程测序结果。将cDNA克隆的序列与GenBank数据库中的序列进行比较。808个克隆（44.4%）似乎是完全未知的，并且可能代表新描述的基因，而110个克隆（55.6%）基于与数据库中的序列的匹配而被鉴定。结果包括α珠蛋白、几种核糖体蛋白、α胶原、热休克蛋白90和细胞色素氧化酶亚基等基因。从肾脏中鉴定的免疫相关cDNA为免疫球蛋白重链和轻链、β2-微球蛋白、胸腺素β和溶菌酶、MHC 11α类。

项目成果

Kono,T.,M.Sakai,S.E.,Lapatra: "Expressod sequence tug analysis of kidney and gill tissues from rainbow trout concorhynchas mykiss 1 infected Rematop oietic mecrosis"Marine Biotechnology. 2(2). 493-498 (2000)

Kono，T.，M.Sakai，S.E.，Lapatra：“对虹鳟鱼 concorhynchas mykiss 1 感染的 Rematop oietic mecrosis 的肾脏和鳃组织进行表达序列拖拽分析”海洋生物技术。

Kono,T.M.Sakai,S.E.Lapatra: "Expressed sequencetay analysis if Kidney and gill tissues from rainbia tnct (oncorhyn, chus myniss) implicated hematopoietic necrosis"Marine Biotechnology. 2(4). 493-498 (2000)

Kono，T.M.Sakai，S.E.Lapatra：“Rainbia tnct（oncorhyn，chus myniss）的肾和鳃组织是否涉及造血坏死的表达序列分析”海洋生物技术。

Kono, T., M.Sakai, S.E.LaPatra: "Expressed sequence tag analysis of kidney and gill tissues from rainbow trout (Oncorhynchus mykiss) infected with infectious hematopoetic necrosis virus."Marine Biotechnology. 2 (4). 493-498 (2000)

Kono, T.、M.Sakai、S.E.LaPatra：“对感染传染性造血坏死病毒的虹鳟鱼 (Oncorhynchus mykiss) 的肾脏和鳃组织进行表达序列标签分析。”海洋生物技术。

Kono,T.,T.Oshikawa,M.Sakai: "The analysis of expressed genes in kidney of Japanese hlounder, Paralicht-hys olivaceus injected with immunistimulant pop tiglycas."Fish and Shellfish Immunology. 11(発表予定). (2001)

Kono, T., T. Oshikawa, M. Sakai：“注射免疫剂 pop tiglycas 的日本猎犬肾中表达基因的分析。”鱼类和贝类免疫学 11（即将发表）。 ）

Kono,T.,M.Sakai: "The analysis of expressed genes in kidney of Japanese flounder, Paralilithys olivacous injected with immunostimulant peptidoylycan"Fish and Shellfish Immunology. 11(発表予定). (2001)

Kono，T.，M. Sakai：“注射免疫刺激剂肽聚糖的日本牙鲆肾中表达基因的分析”鱼类和贝类免疫学 11（即将发表）。