Molecular phylogenic study of Ehrlichia spp. microorganisms as the etiological agents of emerging diseases in Japan

1)在日本最北部岛屿北海道莱姆病流行区捕获的小型啮齿动物中检测到埃里希体基因。以埃立克体属168 rDNA为靶区，以16S rDNA的其它区域为靶区，设计引物。chaffeensis和E.鼠被用于识别。用PCR方法扩增了E. spp.在94只姬鼠中有4只检出了HSP70。73只棕腹鼠中有5只阳性，阳性率为6.8%。扩增的168 rDNA片段的核苷酸序列与来自日本东北部的鼠样埃立克体、埃立克体HF565和静冈36的核苷酸序列最相似。根据16S rDNA序列分析，东北群和西南群E. murrslike埃立克体与E.鼠属微生物。这些结果表明，E. 2）在苏丹发现了一种新的基因型的瘤胃埃里希体（Ehrlichia rummantium），它是心脏病的病原体。新的基因型被暂时命名为“Gedaref”。对pCS 20和主要抗原蛋白I（map）基因区域的同源性分析结果表明，该埃立克体属E. ruminantium群，但不与任何已知的E.反刍动物。此外，根据遗传图谱分析结果，“Gedaref”与多株E. Ruminantium而不是Um Banein，后者曾被认为是E.反刍动物在苏丹。这项工作提供的证据表明，不仅Um Banein菌株，但也存在于苏丹的其他菌株。

1)Ehrlichial gene was detected from small rodents trapped in a Lyme disease-endemic area in Hokkaido, the northernmost island of Japan. Primer pairs of 168 rDNA targeting the genus of Ehrlichia and other region of the 16S rDNA specific for E. chaffeensis and E. muris were used for identification. The DNA fragment specific for 16S rDNA of E. spp. was detected from 4 of 94 Apodemus. speciosus mice (positive rate 4.3%) and 5 of 73 Clethrionomys rufocanus bedfordiae mice (positive rate 6.8%). The nucleotide sequence of the amplified 168 rDNA fragment was most similar to those of E muris-like ehrlichia, E spp HF565 and Shizuoka-36 originating in the northeastern part of Japan. In phylogenic analysis based on 16S rDNA sequences, these northeastern and southwestern groups of E. murrslike ehrlichia made a cluster with E. muris group microorganisms. These results suggest that there are two groups of E. murislike microorganisms in Japan.2)A novel genotype of Ehrlichia rummantium, the causative agent of heartwater, was genetically detected in the Sudan. The new genotype was provisionally named as "Gedaref". The results of phylogenic analyses on the regions of pCS20 and major antigen protein I (map)) genes revealed that the ehrlichia) strain belongs to E. ruminantium-group, but does not correspond with any known strains of E. ruminantium. Furthermore, as the results of phylogenic analyses on map), "Gedaref" made clusters with several strains of E. ruminantium other than Um Banein, which had been known as the sole strain of E. ruminantium in the Sudan. This work provided the evidence that not only Um Banein strain but also the other strain exist in the Sudan.