Molecular phylogenic study of Ehrlichia spp. microorganisms as the etiological agents of emerging diseases in Japan

埃里希体的分子系统发育研究。

• 批准号: 14560272
• 负责人: MURAMATSU Yasukazu
• 金额: $ 2.3万
• 依托单位: RAKUNO GAKUEN UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560272/
• 关键词: Ehrlichia muris-like microorganism; wild rodents; Hokkaido; Japan; Ehrlichia ruminantium; Heartwater; Sudan; novel eenotvoe; Ehrlichia種; 野ネズミ; Amblyomma spp.マダニ; 新興感染症; エーリキア; マダニ; DNA

1)Ehrlichial gene was detected from small rodents trapped in a Lyme disease-endemic area in Hokkaido, the northernmost island of Japan. Primer pairs of 168 rDNA targeting the genus of Ehrlichia and other region of the 16S rDNA specific for E. chaffeensis and E. muris were used for identification. The DNA fragment specific for 16S rDNA of E. spp. was detected from 4 of 94 Apodemus. speciosus mice (positive rate 4.3%) and 5 of 73 Clethrionomys rufocanus bedfordiae mice (positive rate 6.8%). The nucleotide sequence of the amplified 168 rDNA fragment was most similar to those of E muris-like ehrlichia, E spp HF565 and Shizuoka-36 originating in the northeastern part of Japan. In phylogenic analysis based on 16S rDNA sequences, these northeastern and southwestern groups of E. murrslike ehrlichia made a cluster with E. muris group microorganisms. These results suggest that there are two groups of E. murislike microorganisms in Japan.2)A novel genotype of Ehrlichia rummantium, the causative agent of heartwater, was genetically detected in the Sudan. The new genotype was provisionally named as "Gedaref". The results of phylogenic analyses on the regions of pCS20 and major antigen protein I (map)) genes revealed that the ehrlichia) strain belongs to E. ruminantium-group, but does not correspond with any known strains of E. ruminantium. Furthermore, as the results of phylogenic analyses on map), "Gedaref" made clusters with several strains of E. ruminantium other than Um Banein, which had been known as the sole strain of E. ruminantium in the Sudan. This work provided the evidence that not only Um Banein strain but also the other strain exist in the Sudan.

1)从日本最北端岛屿北海道莱姆病流行区捕获的小型啮齿动物中检测到埃立克病毒基因。用168对针对埃立克体属和16S rDNA中查菲埃希氏菌和野鼠埃希氏菌特异的16S rDNA的引物进行鉴定。E.spp.16S rDNA特异DNA片段从94只姬鼠中检出4只。异种鼠阳性率为4.3%，73只红纹伊蚊阳性5只，阳性率为6.8%。扩增出的168个rDNA片段与日本东北部的类埃立克体、E spp HF565和静冈-36的核苷酸序列最为相似。在基于16S rDNA序列的系统发育分析中，东北和西南部的类E.Murrs型埃立克体群与E.Muris群的微生物聚在一起。这些结果表明，在日本存在两组E-Murislike微生物。2)在苏丹发现了一种新的基因型鲁曼埃立克氏菌，它是心脏水的病原体。新的基因型暂时命名为“Gedaref”。对pCS20和主要抗原蛋白I(MAP)基因的系统发育分析结果表明，该菌株属于瘤胃埃希氏菌群，但与已知的任何一株瘤胃埃希氏菌不一致。此外，根据MAP上的系统发育分析结果，“Gedaref”与除Um Banein之外的几个瘤胃肠球菌菌株聚在一起，而Um Banein菌株被认为是苏丹唯一的瘤胃肠球菌菌株。这项工作提供了证据，证明苏丹不仅存在Um Banein菌株，而且还存在另一种菌株。

项目成果

Detection of Ehrlichial DNA from small rodents captured in a woodland located in an area of Hokkaido, the northernmost island of Japan, in which Lyme disease is endemic.

在莱姆病流行的日本最北端岛屿北海道地区的林地中捕获的小型啮齿动物中检测到埃利希氏体 DNA。

• 发表时间: 2005
• 期刊: Jpn J Infect Dis.(Receipt No.; MS2739) (Under submission)
• 作者: Muramatsu Y;Ikeda E;Morita C;Tamura Y
• 通讯作者: Tamura Y

A novel genotype of Ehrlichia ruminantium detected in Amblyomma spp. ticks in the Sudan.

在 Amblyomma spp 中检测到反刍埃里希体的新基因型。

• 发表时间: 2005
• 期刊: Emerg Infect Dis. (In preparation)
• 作者: Muramatsu Y;Ukegawa S;EI Hussein AR;Rahman MBA;Gabbar KMAA;Chitambo AM;Komiya T;Mwase ET;Morita C;Tamura Y
• 通讯作者: Tamura Y