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Differential function of antigen-presenting cells induced by IL-3 in host protective immunity against Trichinella spiralis infection

IL-3诱导的抗原呈递细胞在宿主对抗旋毛虫感染的保护性免疫中的差异功能

基本信息

批准号：
14570216
负责人：
KORENAGA Masataka
金额：
$ 1.92万
依托单位：
Kochi University(Medical School)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570216/
关键词：
Trichinella spiralis IL-3 IL-4 IL-10 IL-2 CD11b+ cells protective immunity Mac1陽性細胞 Th2 マスト細胞小腸粘膜

项目摘要

We have reported that both Th2 responses and protective immunity were accelerated in mice treated with rIL-3 after an infection of Trichinella spiralis. It has been unclear how IL-3 predisposes the host's immune system to Th2 dominance in the early stage of the infection. In the present study, we analyzed the cells that could transfer both protective immunity and Th2 responses to naive C3H/He mice. The spleen cells were isolated from mice treated with rIL-3. One day after the cell transfer, mice were challenged with 400 muscle larvae of T.spiralis. The result showed that protective immunity induced by the cell transfer was dependent on the number of the cells transferred. When the spleen cells were incubated in plastic petri dishes, adherent cells could transfer the immunity to normal mice but not non-adherent cells. Furthermore, we separated the cells, using anti-CD11b and anti-CD45R monoclonal antibodies(mAbs). Adult worm burden in mice transferred with CD11b_+ cells was significantly less than that in control. Spleen cells of the challenged mice were isolated and cultured under the stimulation of anti-CD3 mAb. We measured cytokines in the supernatant of the cell culture. IL-4 production in spleen cells from mice transferred with CD11b_+ cells was significantly higher than that of control. Although there was no significant difference of IL-2 among the groups, the cells from mice transferred with CD11b_+ cells produced less IL-10. These results suggest that IL-3 acts on CD11b_+ cells and regulates protective immunity through differential expression of Th2 cytokines in mice infected with T.spiralis.

我们已经报道了在旋毛虫感染后用rIL-3处理的小鼠中，Th 2应答和保护性免疫都被加速。目前尚不清楚IL-3如何在感染的早期使宿主的免疫系统倾向于Th 2优势。在本研究中，我们分析了可以将保护性免疫和Th 2应答传递给幼稚C3 H/He小鼠的细胞。从用rIL-3处理的小鼠中分离脾细胞。细胞转移后一天，用400个旋毛虫肌幼虫攻击小鼠。结果表明，细胞转移诱导的保护性免疫与转移细胞的数量有关。脾细胞在塑料培养皿中培养时，贴壁细胞能将免疫力传递给正常小鼠，而非贴壁细胞则不能。此外，我们使用抗CD 11b和抗CD 45 R单克隆抗体（mAb）分离细胞。移植CD 11b+细胞的小鼠成虫负荷明显低于对照组。分离受攻击小鼠的脾细胞，在抗CD 3单抗刺激下培养。我们测量了细胞培养物上清液中的细胞因子。CD 11b+细胞转染小鼠脾细胞产生IL-4的能力明显高于对照组。虽然各组间IL-2无明显差异，但转移CD 11b_+细胞的小鼠细胞产生的IL-10较少。提示IL-3作用于CD 11b_+细胞，通过Th 2细胞因子的差异表达调节旋毛虫感染小鼠的保护性免疫。