Distribution of metallo-β-lactamase genes among clinically isolated strains of Gram-negative-rods and nosocomial infection control

革兰氏阴性杆菌临床分离株金属β-内酰胺酶基因分布及医院感染控制

• 批准号: 14570235
• 负责人: ITO Hideo
• 金额: $ 2.24万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570235/
• 关键词: Gram-negative-rods; metallo-β-lactamase; sodium mercaptoacetic acid (SMA) disk method; nosocomial infection; polymerase chain reaction (PCR) analysis; pulsed field gel electrophoresis; (PFGE) analysis; Extender spectrumβ-lactamase (ESBL)

The prevalence of strains producing metallo-β-lactamase among 207 high-level ceftazidime-resistant gram-negative rods (GNRs) was investigated. The strains were isolated in twelve general hospitals in Aichi Prefecture through March 2002 and March 2003. Disk diffusion method using sodium mercapto-acetic acid (SMA) revealed that 60 of them were metallo-β-lactamase producers. The prevalence increased 10.3% in 1997,23.0% in 2001 to 29.0% in this study, and the increase was remarkable among species other than Psudomonas aeruginosa and Serratia marcescens. PCR analysis confirmed that all of the 60 metallo-β-lactamase producing strains detected by SMA disk diffusion method carried the bla_<IMP-1> gene. In this study, SMA disk diffusion method proved useful in detecting metallo-β-lactamase producing at low cost.In one hospital Extended-spectrum-β-lactamases (ESBLs) producing 13 strains (9 Escherichia coli and 4 Klebsiella oxytoca) were isolated in the internal ward and neonatal intensive care unit (NICU). By pulsed field gel electrophresis (PFGE) each 6 of the 9 strains and each 3 of 4 strains showed the same restriction pattern.In another hospital 9 methicillin-resistant Staphylococcus aureus (MRSA) strains were isolated in the intensive care unit (ICU). By PFGE 8 of 9 strains were showed the same genotype.

对207株高水平头孢他啶耐药革兰氏阴性棒（gnr）产金属β-内酰胺酶菌株的流行情况进行了调查。2002年3月至2003年3月，在爱知县的12家综合医院分离出该菌株。巯基乙酸钠（SMA）圆盘扩散法发现，其中60株为金属β-内酰胺酶产生菌。在本研究中，感染率从1997年的10.3%上升到2001年的23.0%至29.0%，除铜绿假单胞菌和粘质沙雷菌外，其他物种的感染率均有显著上升。PCR分析证实，用SMA圆盘扩散法检测到的60株金属β-内酰胺酶产菌均携带bla_<IMP-1>基因。在本研究中，SMA圆盘扩散法被证明可以低成本地检测金属β-内酰胺酶的产生。在某医院的内科病房和新生儿重症监护病房（NICU）分离到产生13株（9株大肠杆菌和4株产氧克雷伯菌）的广谱β-内酰胺酶（ESBLs）。通过脉冲场凝胶电泳（PFGE）检测，9株菌株中6株和4株菌株中3株均表现出相同的酶切模式。在另一家医院重症监护病房（ICU）分离出9株耐甲氧西林金黄色葡萄球菌（MRSA）。经PFGE检测，9株菌株中8株基因型一致。

项目成果

Hideo Ito, et al.: "Distribution of metallo-β-lactamase genes among clinically isolated strains of Gram-Negative Rods."Journal of Japanese Clinical Microbiology. 12. 93-98 (2002)

Hideo Ito 等人：“临床分离的革兰氏阴性杆菌菌株中金属-β-内酰胺酶基因的分布”。日本临床微生物学杂志 12. 93-98 (2002)。

伊藤秀郎: "メタロβ-ラクタマーゼ産生菌の分布調査と臨床的意義"日本臨床微生物学雑誌. 12巻・2号. 93-98 (2002)

Hideo Ito：“金属-β-内酰胺酶产生菌的分布调查和临床意义”，《日本临床微生物学杂志》，第 12 卷，第 2 期，93-98（2002 年）。

Jun-ichi Wachino: "Nosocomial Spread of Ceftazidime-Resistant Klebsiella pneumoniae Strains Producing a Novel Class A β-Lactamse, GES-3, in a Neonatal ICU in Japan"Antimicrobial Agents and Chemotherapy. (in press). (2004)

Jun-ichi Wachino：“日本新生儿 ICU 中产生新型 A 类 β-内酰胺酶 GES-3 的头孢他啶耐药肺炎克雷伯菌菌株的医院传播”抗菌药物和化疗（2004 年出版）。

Jun-ichi Wachino: "Nosocomial Spread of Ceftazidime-Resistant Klebsiella pneumoniae Strains Producing a Novel Class A β-Lactamase, GES-3, in a Neonatal ICU in Japan."Antimicrobial Agents and Chemotherapy. (in press). (2004)

Jun-ichi Wachino：“日本新生儿 ICU 中产生新型 A 类 β-内酰胺酶 GES-3 的头孢他啶耐药肺炎克雷伯菌菌株的医院传播”。抗菌药物和化疗（2004 年出版）。

Jun-ichi Wachino, et al.: "Nosocomial Spread of Ceftazidime-Resistant Klebsiella pneumoniae Strains Producing a Novel Class A β-lactamase, GES-3,in Neonatal ICU in Japan."Antimicrobial Agents and Chemotherapy. (In press). (2004)

Jun-ichi Wachino 等人：“日本新生儿 ICU 中产生新型 A 类 β-内酰胺酶 GES-3 的头孢他啶耐药肺炎克雷伯菌菌株的医院传播”。抗菌药物和化疗（印刷中）。 ）