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Wound Age Determination of Bruises Using Immunohistological and Molecular Biological Assays.

使用免疫组织学和分子生物学测定确定瘀伤的伤口年龄。

基本信息

批准号：
14570395
负责人：
AOKI Yasuhiro
金额：
$ 1.34万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570395/
关键词：
bruise wound aging wound healing tPA fibronectin quantitative PCR in situ hybridization forensic pathology サイトカイン遺伝子発現 plasminogen

项目摘要

An animal experimental study was carried out to investigate availability of mRNA expressions of tissue-type plasminogen activator (tPA) and fibronectin, and inflammatory cell dynamics for wound age estimation of bruises. The bruises ware made by compressing the dorsal skin of mice with 2 iron plates with holes, 3mm in diameter. The mice were killed at 1, 3, 8, 24, 72, 144, or 240 hours post-injury. tPA mRNA expression peaked at 1 hour, and increased slightly at 72 hours post-injury. It was detected in epidermal cells, fibroblasts, and endothelial cells before and after injury, and from 3 hours in neutrophils and from 72 hours in macrophages, respectively. tPA was considered to play an essential role in the first step of tissue remodeling. A slight increase of tPA mRNA expression at later phase might be related to epidermal proliferation. On the other hand, expression of fibronectin mRNA was biphasic. It peaked at 1 hour, and significantly increase at 144 hours post-injury. Acute increase of fibronection would be due to tissue reaction of mechanical injury, and activation of coagulation cascade. Proriferation of the fibroblasts would be major cause of the late phase peak. Examination of the correlation of microscopic changes, such as inflammatory cell dynamics and epidermal thickening, with mRNA expression of tPA, fibronectin and other cytokines, would be informative for bruise healing and dating in the acute and chronic phases.

进行了一项动物实验研究，以研究组织型纤溶酶原激活剂 (tPA) 和纤连蛋白 mRNA 表达的可用性，以及炎症细胞动力学用于估计瘀伤的伤口年龄。瘀伤是用2块直径为3毫米的带孔铁板压迫小鼠背部皮肤制成的。小鼠在受伤后 1、3、8、24、72、144 或 240 小时处死。 tPA mRNA 表达在损伤后 1 小时达到峰值，并在损伤后 72 小时略有增加。在损伤前后的表皮细胞、成纤维细胞和内皮细胞中以及中性粒细胞中 3 小时和巨噬细胞中 72 小时中分别检测到它。 tPA被认为在组织重塑的第一步中发挥着重要作用。后期tPA mRNA表达略有增加可能与表皮增殖有关。另一方面，纤连蛋白mRNA的表达是双相的。它在受伤后 1 小时达到峰值，并在受伤后 144 小时显着增加。纤维连接的急剧增加是由于机械损伤的组织反应和凝血级联的激活。成纤维细胞的增殖是晚期峰的主要原因。检查微观变化（例如炎症细胞动力学和表皮增厚）与 tPA、纤连蛋白和其他细胞因子的 mRNA 表达的相关性，将为急性和慢性阶段的瘀伤愈合和约会提供信息。