Wound Age Determination of Bruises Using Immunohistological and Molecular Biological Assays.

使用免疫组织学和分子生物学测定确定瘀伤的伤口年龄。

基本信息

  • 批准号:
    14570395
  • 负责人:
  • 金额:
    $ 1.34万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2003
  • 项目状态:
    已结题

项目摘要

An animal experimental study was carried out to investigate availability of mRNA expressions of tissue-type plasminogen activator (tPA) and fibronectin, and inflammatory cell dynamics for wound age estimation of bruises. The bruises ware made by compressing the dorsal skin of mice with 2 iron plates with holes, 3mm in diameter. The mice were killed at 1, 3, 8, 24, 72, 144, or 240 hours post-injury. tPA mRNA expression peaked at 1 hour, and increased slightly at 72 hours post-injury. It was detected in epidermal cells, fibroblasts, and endothelial cells before and after injury, and from 3 hours in neutrophils and from 72 hours in macrophages, respectively. tPA was considered to play an essential role in the first step of tissue remodeling. A slight increase of tPA mRNA expression at later phase might be related to epidermal proliferation. On the other hand, expression of fibronectin mRNA was biphasic. It peaked at 1 hour, and significantly increase at 144 hours post-injury. Acute increase of fibronection would be due to tissue reaction of mechanical injury, and activation of coagulation cascade. Proriferation of the fibroblasts would be major cause of the late phase peak. Examination of the correlation of microscopic changes, such as inflammatory cell dynamics and epidermal thickening, with mRNA expression of tPA, fibronectin and other cytokines, would be informative for bruise healing and dating in the acute and chronic phases.
本研究通过动物实验研究了组织型纤溶酶原激活物(tPA)和纤维连接蛋白(FN)mRNA表达及炎症细胞动力学对创伤时间推断的应用价值。用2块直径3mm的带孔铁板压迫小鼠背部皮肤造成挫伤。在损伤后1、3、8、24、72、144或240小时处死小鼠。tPA mRNA表达于伤后1 h达高峰,72 h略有增加。在损伤前后的表皮细胞、成纤维细胞和内皮细胞中检测到,并且分别在中性粒细胞中从3小时起和巨噬细胞中从72小时起检测到。tPA被认为在组织重塑的第一步中起重要作用。tPA mRNA表达后期略有增加,可能与表皮增生有关。另一方面,纤连蛋白mRNA的表达呈双相性。伤后1h达高峰,144h明显升高。纤维连接蛋白的急性升高可能是由于机械损伤的组织反应和凝血级联反应的激活。成纤维细胞的增殖是晚期峰值的主要原因。检查显微镜下的变化,如炎症细胞动力学和表皮增厚,与tPA,纤连蛋白和其他细胞因子的mRNA表达的相关性,将是在急性和慢性期瘀伤愈合和约会的信息。

项目成果

期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takamiya M.Saigusa K, Aoki Y.: "Immunohistrochemical study of bFGF and VEGF expression for age determination of cutaneous wounds"American Journal of Forensic Medicine and Pathology. 23・3. 264-267 (2002)
Takamiya M. Saigusa K,Aoki Y.:“bFGF 和 VEGF 表达的免疫组织化学研究用于确定皮肤伤口的年龄”美国法医学和病理学杂志 23・3 (2002)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Takamiya M, Saigusa K, Nakayashiki N, Aoki Y: "Studies on mRNA expression of basic fibroblast growth factor in wound healing for wound age determination"International Journal of Legal Medicine. 117・1. 46-50 (2003)
Takamiya M、Saigusa K、Nakayashiki N、Aoki Y:“伤口愈合中碱性成纤维细胞生长因子的 mRNA 表达用于确定伤口年龄”国际法律医学杂志 117・1(2003 年)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Studies on mRNA expressions of tissue-type plasminogen activator in bruises for wound age estimation.
瘀伤中组织型纤溶酶原激活剂 mRNA 表达的研究用于伤口年龄估计。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Takamiya M;Saigusa K;Nakayashiki N;Aoki Y.
  • 通讯作者:
    Aoki Y.
Studies on mRNA expressions of basic fibroblast growth factor during dermal, cerebral, renal, and hepatic wound healing for wound age determination
真皮、大脑、肾脏和肝脏伤口愈合过程中碱性成纤维细胞生长因子 mRNA 表达的研究,用于确定伤口年龄
A case of acute gasoline intoxication at the scene of washing a petrol tank.
一宗在清洗油箱现场发生的急性汽油中毒个案。
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Takamiya M;Niitsu N;Saigusa K;Kanetake J;Aoki Y.
  • 通讯作者:
    Aoki Y.
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AOKI Yasuhiro其他文献

AOKI Yasuhiro的其他文献

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{{ truncateString('AOKI Yasuhiro', 18)}}的其他基金

Morphometric age and sex estimation of the pelvis by three dimensional analysis of the homologous models.
通过同源模型的三维分析来估计骨盆的形态年龄和性别。
  • 批准号:
    19K10690
  • 财政年份:
    2019
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies on age and sex dependent morphological changes of the coxal bone by three dimensional analysis of the homologous models.
通过同源模型的三维分析研究髋骨的年龄和性别依赖性形态变化。
  • 批准号:
    16K09213
  • 财政年份:
    2016
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A research on the rent of the US military bases in Okinawa prefecture
冲绳县美军基地租金问题研究
  • 批准号:
    23653139
  • 财政年份:
    2011
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Three dimensional analysis of pubic bone and pelvic auricular surfaces using reconstructed CT images.
使用重建 CT 图像对耻骨和骨盆耳廓表面进行三维分析。
  • 批准号:
    22590640
  • 财政年份:
    2010
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Dermal wound aging by molecular biological detections of growth factors
通过生长因子的分子生物学检测真皮伤口老化
  • 批准号:
    18590642
  • 财政年份:
    2006
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Asurvey research on policy problems some remote islands and small local communities are facing in the changing Japanese society
日本社会变迁中一些偏远岛屿和地方小社区面临的政策问题的调查研究
  • 批准号:
    18330114
  • 财政年份:
    2006
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Studies on mRNA Expressions of Cytokines during Wound Healing for Determination of Wound Age.
研究伤口愈合过程中细胞因子 mRNA 表达以确定伤口年龄。
  • 批准号:
    12670402
  • 财政年份:
    2000
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Study on the social structure and political culture of local communities
当地社区的社会结构和政治文化研究
  • 批准号:
    10301011
  • 财政年份:
    1998
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Immunohistochemical and Ultrastructual Study of Brain Injury Produced by Repeated Impacts.
反复撞击造成的脑损伤的免疫组织化学和超微结构研究。
  • 批准号:
    09670450
  • 财政年份:
    1997
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Composition and change of Imperial Diet Members
帝国国会议员的构成及变动
  • 批准号:
    04451046
  • 财政年份:
    1992
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

相似海外基金

Dermal wound aging by molecular biological detections of growth factors
通过生长因子的分子生物学检测真皮伤口老化
  • 批准号:
    18590642
  • 财政年份:
    2006
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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