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Wound Age Determination of Bruises Using Immunohistological and Molecular Biological Assays.

使用免疫组织学和分子生物学测定确定瘀伤的伤口年龄。

基本信息

批准号：
14570395
负责人：
AOKI Yasuhiro
金额：
$ 1.34万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570395/
关键词：
bruise wound aging wound healing tPA fibronectin quantitative PCR in situ hybridization forensic pathology サイトカイン遺伝子発現 plasminogen

项目摘要

An animal experimental study was carried out to investigate availability of mRNA expressions of tissue-type plasminogen activator (tPA) and fibronectin, and inflammatory cell dynamics for wound age estimation of bruises. The bruises ware made by compressing the dorsal skin of mice with 2 iron plates with holes, 3mm in diameter. The mice were killed at 1, 3, 8, 24, 72, 144, or 240 hours post-injury. tPA mRNA expression peaked at 1 hour, and increased slightly at 72 hours post-injury. It was detected in epidermal cells, fibroblasts, and endothelial cells before and after injury, and from 3 hours in neutrophils and from 72 hours in macrophages, respectively. tPA was considered to play an essential role in the first step of tissue remodeling. A slight increase of tPA mRNA expression at later phase might be related to epidermal proliferation. On the other hand, expression of fibronectin mRNA was biphasic. It peaked at 1 hour, and significantly increase at 144 hours post-injury. Acute increase of fibronection would be due to tissue reaction of mechanical injury, and activation of coagulation cascade. Proriferation of the fibroblasts would be major cause of the late phase peak. Examination of the correlation of microscopic changes, such as inflammatory cell dynamics and epidermal thickening, with mRNA expression of tPA, fibronectin and other cytokines, would be informative for bruise healing and dating in the acute and chronic phases.

通过动物实验研究组织型纤溶酶原激活物(TPA)和纤维连接蛋白(FN)的基因表达及炎性细胞动力学在判断挫伤创面年龄中的作用。这些瘀伤是用直径3 mm的2块有孔的铁板压迫小鼠背部皮肤造成的。分别于伤后1、3、8、24、72、144或240小时处死小鼠。TPA mRNA表达于伤后1h达高峰，72h略有升高。在损伤前、损伤后的表皮细胞、成纤维细胞和内皮细胞中，中性粒细胞和巨噬细胞分别从3h和72h开始表达。TPA被认为在组织重建的第一步中起着至关重要的作用。晚期tPA mRNA表达略有增加，可能与表皮增殖有关。另一方面，纤维连接蛋白mRNA的表达呈双相。伤后1小时达高峰，伤后144小时显著升高。纤维连接蛋白的急性升高可能是由于机械损伤的组织反应和凝血级联的激活所致。成纤维细胞的活化可能是晚期峰值的主要原因。检测炎症细胞动力学、表皮增厚等微观变化与tPA、纤维连接蛋白等细胞因子mRNA表达的相关性，将有助于急性期和慢性期挫伤的愈合和年代测定。