Wound Age Determination of Bruises Using Immunohistological and Molecular Biological Assays.
使用免疫组织学和分子生物学测定确定瘀伤的伤口年龄。
基本信息
- 批准号:14570395
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
An animal experimental study was carried out to investigate availability of mRNA expressions of tissue-type plasminogen activator (tPA) and fibronectin, and inflammatory cell dynamics for wound age estimation of bruises. The bruises ware made by compressing the dorsal skin of mice with 2 iron plates with holes, 3mm in diameter. The mice were killed at 1, 3, 8, 24, 72, 144, or 240 hours post-injury. tPA mRNA expression peaked at 1 hour, and increased slightly at 72 hours post-injury. It was detected in epidermal cells, fibroblasts, and endothelial cells before and after injury, and from 3 hours in neutrophils and from 72 hours in macrophages, respectively. tPA was considered to play an essential role in the first step of tissue remodeling. A slight increase of tPA mRNA expression at later phase might be related to epidermal proliferation. On the other hand, expression of fibronectin mRNA was biphasic. It peaked at 1 hour, and significantly increase at 144 hours post-injury. Acute increase of fibronection would be due to tissue reaction of mechanical injury, and activation of coagulation cascade. Proriferation of the fibroblasts would be major cause of the late phase peak. Examination of the correlation of microscopic changes, such as inflammatory cell dynamics and epidermal thickening, with mRNA expression of tPA, fibronectin and other cytokines, would be informative for bruise healing and dating in the acute and chronic phases.
通过动物实验研究组织型纤溶酶原激活物(TPA)和纤维连接蛋白(FN)的基因表达及炎性细胞动力学在判断挫伤创面年龄中的作用。这些瘀伤是用直径3 mm的2块有孔的铁板压迫小鼠背部皮肤造成的。分别于伤后1、3、8、24、72、144或240小时处死小鼠。TPA mRNA表达于伤后1h达高峰,72h略有升高。在损伤前、损伤后的表皮细胞、成纤维细胞和内皮细胞中,中性粒细胞和巨噬细胞分别从3h和72h开始表达。TPA被认为在组织重建的第一步中起着至关重要的作用。晚期tPA mRNA表达略有增加,可能与表皮增殖有关。另一方面,纤维连接蛋白mRNA的表达呈双相。伤后1小时达高峰,伤后144小时显著升高。纤维连接蛋白的急性升高可能是由于机械损伤的组织反应和凝血级联的激活所致。成纤维细胞的活化可能是晚期峰值的主要原因。检测炎症细胞动力学、表皮增厚等微观变化与tPA、纤维连接蛋白等细胞因子mRNA表达的相关性,将有助于急性期和慢性期挫伤的愈合和年代测定。
项目成果
期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takamiya M.Saigusa K, Aoki Y.: "Immunohistrochemical study of bFGF and VEGF expression for age determination of cutaneous wounds"American Journal of Forensic Medicine and Pathology. 23・3. 264-267 (2002)
Takamiya M. Saigusa K,Aoki Y.:“bFGF 和 VEGF 表达的免疫组织化学研究用于确定皮肤伤口的年龄”美国法医学和病理学杂志 23・3 (2002)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Takamiya M, Saigusa K, Nakayashiki N, Aoki Y: "Studies on mRNA expression of basic fibroblast growth factor in wound healing for wound age determination"International Journal of Legal Medicine. 117・1. 46-50 (2003)
Takamiya M、Saigusa K、Nakayashiki N、Aoki Y:“伤口愈合中碱性成纤维细胞生长因子的 mRNA 表达用于确定伤口年龄”国际法律医学杂志 117・1(2003 年)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Studies on mRNA expressions of tissue-type plasminogen activator in bruises for wound age estimation.
瘀伤中组织型纤溶酶原激活剂 mRNA 表达的研究用于伤口年龄估计。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Takamiya M;Saigusa K;Nakayashiki N;Aoki Y.
- 通讯作者:Aoki Y.
Studies on mRNA expressions of basic fibroblast growth factor during dermal, cerebral, renal, and hepatic wound healing for wound age determination
真皮、大脑、肾脏和肝脏伤口愈合过程中碱性成纤维细胞生长因子 mRNA 表达的研究,用于确定伤口年龄
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Takamiya M;Saigusa K;Nakayashiki N;Aoki Y.
- 通讯作者:Aoki Y.
A case of acute gasoline intoxication at the scene of washing a petrol tank.
一宗在清洗油箱现场发生的急性汽油中毒个案。
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Takamiya M;Niitsu N;Saigusa K;Kanetake J;Aoki Y.
- 通讯作者:Aoki Y.
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AOKI Yasuhiro其他文献
AOKI Yasuhiro的其他文献
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{{ truncateString('AOKI Yasuhiro', 18)}}的其他基金
Morphometric age and sex estimation of the pelvis by three dimensional analysis of the homologous models.
通过同源模型的三维分析来估计骨盆的形态年龄和性别。
- 批准号:
19K10690 - 财政年份:2019
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Studies on age and sex dependent morphological changes of the coxal bone by three dimensional analysis of the homologous models.
通过同源模型的三维分析研究髋骨的年龄和性别依赖性形态变化。
- 批准号:
16K09213 - 财政年份:2016
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A research on the rent of the US military bases in Okinawa prefecture
冲绳县美军基地租金问题研究
- 批准号:
23653139 - 财政年份:2011
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Three dimensional analysis of pubic bone and pelvic auricular surfaces using reconstructed CT images.
使用重建 CT 图像对耻骨和骨盆耳廓表面进行三维分析。
- 批准号:
22590640 - 财政年份:2010
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Dermal wound aging by molecular biological detections of growth factors
通过生长因子的分子生物学检测真皮伤口老化
- 批准号:
18590642 - 财政年份:2006
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Asurvey research on policy problems some remote islands and small local communities are facing in the changing Japanese society
日本社会变迁中一些偏远岛屿和地方小社区面临的政策问题的调查研究
- 批准号:
18330114 - 财政年份:2006
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on mRNA Expressions of Cytokines during Wound Healing for Determination of Wound Age.
研究伤口愈合过程中细胞因子 mRNA 表达以确定伤口年龄。
- 批准号:
12670402 - 财政年份:2000
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on the social structure and political culture of local communities
当地社区的社会结构和政治文化研究
- 批准号:
10301011 - 财政年份:1998
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Immunohistochemical and Ultrastructual Study of Brain Injury Produced by Repeated Impacts.
反复撞击造成的脑损伤的免疫组织化学和超微结构研究。
- 批准号:
09670450 - 财政年份:1997
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Composition and change of Imperial Diet Members
帝国国会议员的构成及变动
- 批准号:
04451046 - 财政年份:1992
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
相似海外基金
Dermal wound aging by molecular biological detections of growth factors
通过生长因子的分子生物学检测真皮伤口老化
- 批准号:
18590642 - 财政年份:2006
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)














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