Dermal wound aging by molecular biological detections of growth factors

通过生长因子的分子生物学检测真皮伤口老化

• 批准号: 18590642
• 负责人: AOKI Yasuhiro
• 金额: $ 1.73万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590642/
• 关键词: skin; brain; bone; cytokine; growth factor; real-time quantitative PCR; in situ hybridization; beads suspension array; 損傷; beads suspension array; quantitave PCR; エイジング; 創傷治癒; 定量的PCR

Dermal wound age provides forensic pathologists with valuable information for the reconstruction of crime scenes and determination of the cause of death. In this study, cytokine expressions in murine skins were analyzed using immunohistochemical methods, real-time quantitative PCR, and in situ hybridization techniques. Wound age estimation for human dermal wounds was also performed based on quantification of 17 cytokines. The potential parameters would be IL 5, IL 10, IL 12 p 70, IL 13, IL 17, GM-CSF, IFN γ, TNF α in the early phase of dermal wound healing, IL 6, MCP 1 in the middle phase, and IL 1 β, IL 2, IL 4, IL 8, G-CSF, and MIP 1β in the middle and late phases. In addition, an outsourced examination of IL 1 β, IL 4, IL 6, IL 8, IL 10, G-CSF, GM-CSF, IFN γ, MCP1, TNFα, which could be available to any forensic institute, was performed for confirmative purposes. Many factors have been proposed as markers for dermal wound age estimation, but the set of cytokines selected for the outsourced examination in the present study wound be useful in daily forensic practice.

皮肤创伤年龄为法医病理学家重建犯罪现场和确定死因提供了有价值的信息。在这项研究中，细胞因子在小鼠皮肤的表达进行了分析，采用免疫组化方法，实时定量PCR和原位杂交技术。还基于17种细胞因子的定量进行了人皮肤伤口的伤口年龄估计。在皮肤创面愈合早期，IL 5、IL 10、IL 12 p70、IL 13、IL 17、GM CSF、IFN γ、TNF α可能是皮肤创面愈合的重要指标，在创面愈合中期，IL 6、MCP 1可能是皮肤创面愈合的重要指标，在创面愈合中后期，IL 1 β、IL 2、IL 4、IL 8、G CSF、MIP 1β可能是皮肤创面愈合的重要指标。此外，还进行了IL 1 β、IL 4、IL 6、IL 8、IL 10、G-CSF、GM-CSF、IFN γ、MCP 1、TNFα的外包检测，这些检测可供任何法医学研究所使用，用于确认目的。许多因素已被提出作为皮肤伤口年龄估计的标志物，但在本研究中选择的外包检查的细胞因子集将在日常法医实践中有用。

项目成果

A study on mRNA expressions of fibronectin in dermal and cerebral wound healing for wound age estimation.

• DOI: 10.1016/j.legalmed.2006.05.002
• 发表时间: 2006-07
• 期刊: Legal medicine
• 影响因子: 1.5
• 作者: M. Takamiya;R. Kumagai;N. Nakayashiki;Y. Aoki

A case of carbamazepine overdose with focal myocarditis.

卡马西平过量致局灶性心肌炎一例。

• 发表时间: 2006
• 期刊: Legal Medicine 8
• 作者: Masataka Takamiya;Yasuhiro Aoki;Hisae Niitsu;Kiyoshi Saigusa
• 通讯作者: Kiyoshi Saigusa

An autopsy case of aortitis resulting in sudden death due to a rupture of aneurysm of the aortic sinus

主动脉炎因主动脉窦瘤破裂猝死尸检一例

• 期刊: Legal Medicine (in press)
• 作者: Yasuhiro Aok;Masataka Takamiya;Hisae Niitsu;Sachiko Fujita;Kiyoshi Saigusa.
• 通讯作者: Kiyoshi Saigusa.

A study on mRNA expressions of tPA and uPA during fracture healing for wound age determination.

骨折愈合过程中 tPA 和 uPA mRNA 表达用于确定伤口年龄的研究。

• 发表时间: 2006
• 作者: Masataka Takamiya;Reiko Kumagai;Nori Nakayashiki;Yasuhiro Aoki
• 通讯作者: Yasuhiro Aoki

骨折治癒過程におけるtPAおよびuPA遺伝子の発現動態

骨折愈合过程中tPA和uPA基因的表达动态

• 发表时间: 2006
• 作者: 高宮正隆;熊谷礼子;中屋敷徳;青木康博.
• 通讯作者: 青木康博.