Studies on mRNA Expressions of Cytokines during Wound Healing for Determination of Wound Age.

研究伤口愈合过程中细胞因子 mRNA 表达以确定伤口年龄。

基本信息

批准号：
12670402
负责人：
AOKI Yasuhiro
金额：
$ 1.73万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670402/
关键词：
injury wound aging wound healing bFGF VEGF fibronectin quantitative PCR in situ hybridization サイトカイン遺伝子発現 fibronectin 免疫組織化学損傷創傷治癒法医学

项目摘要

An animal experimental study was carried out to investigate availability of mRNA expressions of cytekines as markers for wound aging. A 2 cm full thickness incision was made on the dorsal skin of male DDY mice of 5 to 6 weeks old, and they were killed 0.5, 1, 3, 8, 24, 72, 144, or 240 hours after the incision. The expression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) were examined immunohistochemically using specific antibodies. For wound age determination, bFGF, which was detected in the early 0.5- to 1-hour phases and the late 24- to 144-hour phases, seemed to be superior to VEGF because of its earlier expression and because of its more persistent expression in dermal fibroblasts with increasing postomortem interval. Therefore, expressions of bFGF mRNA during wound healing were subsequently analyzed using quantitative PCR and in situ hybridization techniques. Beside the dermal wound, mRNA dynamics of dermal, cerebral, hepatic and renal ones were also examined. bFGF mRNA expressions in the injured skin peaked at 1 hour, and they were detected in epidermal cells, fibroblasts, endothelial cells and neutrophils. Those of the injured cerebrum increased from 1 hour and peaked at 48 hours. The astrocytes were main source of vFGF mRNA supply in the injured brain. In hepatic and renal injuries, however, bFGF mRNA expressions increased slightly at 24 hours in endothelial cells, in neutrophils of the liver, and in glomeruli of the kidney. These results suggested that the mRNA dynamics of skin and brain derived from ectoderm were quite different from those of liver and kidney. In other words, time-dependent expressions of bFGF mRNA could be useful wound aging marker for the organs of ectodermal origin.

进行了一项动物实验研究，以研究Cytekines作为伤口衰老的标志物的mRNA表达的可用性。在切口后，在5至6周大的男性DDY小鼠的背皮上进行了2厘米的全厚切口，在切口后，它们被杀死0.5、1、3、8、24、72、144或240小时。使用特定抗体检查了免疫组织化学的基本成纤维细胞生长因子（BFGF）和血管内皮生长因子（VEGF）的表达。为了确定伤口年龄，BFGF在0.5至1小时早期被检测到的BFGF和24至144小时的早期相位，由于其较早的表达，并且由于其在真皮成纤维细胞中的表达较高，因此似乎优于VEGF，随着其较高的表达，随着邮递的增加而增加。因此，随后使用定量PCR和原位杂交技术对伤口愈合过程中BFGF mRNA的表达进行分析。除皮肤伤口外，还检查了皮肤，脑，肝和肾脏的mRNA动力学。受伤皮肤中的BFGF mRNA表达在1小时峰值，并在表皮细胞，成纤维细胞，内皮细胞和中性粒细胞中检测到它们。受伤的大脑的患者从1小时增加，并在48小时达到峰值。星形胶质细胞是受伤大脑中VFGF mRNA供应的主要来源。然而，在肝和肾脏损伤中，内皮细胞，肝脏中性粒细胞和肾脏的肾小球中，BFGF mRNA表达在24小时时略有增加。这些结果表明，源自外胚层的皮肤和脑的mRNA动力学与肝脏和肾脏的mRNA动力学完全不同。换句话说，BFGF mRNA的时间依赖性表达可能是外皮起源器官的有用伤口老化标记。