the elucidation of the mechanisms of angiogenesia and cardiovascular hypertrophy and the application of angiogenesis by therapeutic electrical stimulation

阐明血管生成和心血管肥大的机制以及治疗性电刺激在血管生成中的应用

• 批准号: 14570634
• 负责人: KOHZUKI Masahiro
• 金额: $ 2.11万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570634/
• 关键词: VEGF; HGF; low voltage electrical stimulation; ischemic muscle

Low voltage electrical stimulation (LVES) of skeletal muscle at a level far below the threshold of muscle contraction has been reported to induce VEGF immunohistochemically but the promotion of local angiogenesis in skeletal muscles by LVES has not been fully elucidated.We tried to evaluate whether angiogenic factors, such as VEGF,HGF and FGF, and other factor, such as inflammation (IL6), and hypoxia (anti HIF-1 α) contribute to the local angiogenesis by LVES. we compared the effect of LVES to that of high voltage electrical stimulation (HVES) on the induction of angiogenic factors. Our results indicated that not only VEGF but also HGF was significantly increased in tissues by LVES. There was no difference in the level of immunohistochemical staining of the tibialis anterior (TA) muscles with polyclonal anti FGF antibody, anti TM antibody, and anti HIF1α antibody between the LVES group and control group. The VEGF level of the intermittent high voltage electrical stimulation (HVES) group tended to be higher than that of the LVES groups, although the difference was not significant. Capillary density of stimulated muscles was increased with the unstimulated muscles in CL-VES and IH-VES. The induction of VEGF by LVES was seen at a significantly earlier period than that by HVES.

在远低于肌肉收缩阈值的水平下对骨骼肌进行低电压电刺激（LVES）已被报道可化学诱导VEGF，但LVES促进骨骼肌局部血管生成的作用尚未完全阐明，我们试图评估血管生成因子，如VEGF、HGF和FGF，以及其他因子，如炎症因子（IL 6），低氧（抗HIF-1 α）可促进LVES局部血管生成。我们比较了LVES和高压电刺激（HVES）对血管生成因子诱导的作用。我们的结果表明，不仅VEGF，而且HGF在LVES组织中显着增加。LVES组和对照组胫骨前肌（TA）的多克隆抗FGF抗体、抗TM抗体和抗HIF 1 α抗体的免疫组化染色水平无差异。间歇高压电刺激组VEGF水平有高于LVES组的趋势，但差异无显著性。在CL-VES和IH-VES中，受刺激肌肉的毛细血管密度与未受刺激的肌肉相比增加。LVES诱导VEGF的时间明显早于HVES。

项目成果

Kohzuki M, et al.: "Disability prevention of renal failure : effects of exercise and enalapril in Thy-1 nephritis rats"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editor, bologna. 52

Kohzuki M 等人：“肾衰竭的残疾预防：运动和依那普利对 Thy-1 肾炎大鼠的影响”国际物理和康复医学会第二届世界大会论文集，Monduzzi 编辑，博洛尼亚。

Kanazawa M, et al.: "Disability prevention of chronic renal failure (CRF) : effects of moderate to intense exercise in rats with CRF"Disability prevention of chronic renal failure (CRF) : effects of moderate to intense exercise in rats with CRF. 517-520 (

Kanazawa M 等人：“慢性肾衰竭（CRF）的残疾预防：中度至剧烈运动对 CRF 大鼠的影响”慢性肾衰竭（CRF）的残疾预防：中度至剧烈运动对 CRF 大鼠的影响。

Chida K: "Ventriculography using ECG-gated multiple diastolic injection of contrast material in pediatric angiocardiography"Pediatr Cardiol. 23. 200-204 (2002)

Chida K：“在儿科心血管造影中使用心电门控多次舒张期注射造影剂进行心室造影”Pediatr Cardiol。

Kawamura T: "Impact of exercise and ACE inhibition on TNF-α and leptin in hypertensive rats"Hypertens Res. 25. 919-926 (2002)

Kawamura T：“运动和 ACE 抑制对高血压大鼠 TNF-α 和瘦素的影响”Hypertens Res. 25. 919-926 (2002)

Kohzuki M, et al.: "Disability prevention of renal failure : effects of exercise and enalapril in Thy-1 nephritis rats"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore. Bologna. 5

Kohzuki M 等人：“肾衰竭的残疾预防：运动和依那普利对 Thy-1 肾炎大鼠的影响”国际物理和康复医学会第二届世界大会论文集，Monduzzi Editore。